ISOLATION AND CHARACTERIZATION OF EXTREMOZYMES FROM ALKALINE LAKES IN NEBRASKA

内布拉斯加州碱性湖泊中极端酶的分离和表征

• 批准号: 8167495
• 负责人: JULIE SHAFFER
• 金额: $ 7.47万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167495
• 关键词: Biotechnology; Cellulases; Communities; Computer Retrieval of Information on Scientific Projects Database; Environment; Enzyme Gene; Enzymes; Escherichia coli; Funding; Grant; Institution; Lipase; Nebraska; Peptide Hydrolases; Reaction; Research; Research Personnel; Resources; Source; Taq Polymerase; Thermus; United States National Institutes of Health; cellulase; enzyme activity; esterase; hyperthermophile; interest; microorganism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Extreme environments have been of considerable interest in recent years due to the discovery that these "uninhabitable" environments are colonized by a diverse community of microorganisms. These microorganisms have been shown to provide valuable extremozymes, enzymes adapted to catalyze reactions under extreme environmental conditions. One well-known extremozyme is Taq polymerase from the hyperthermophile Thermus aquaticus. This research focuses on the little studied, hypersaline, alkaline lakes in western Nebraska. We will isolate and identify potential microorganisms capable of producing extremozymes of interest for numerous biotechnology applications. We will look for esterases, xylanases, cellulases, proteases, and lipases that are active at high pH's. Microorganisms capable of producing these enzymes will be isolated, the enzyme activity characterized, the enzyme purified, and the enzyme gene sequences identified and cloned into Escherichia coli.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 由于发现这些“不可居住的”环境是由多样化的微生物社区殖民的，因此近年来，极端环境引起了极大的兴趣。 这些微生物已被证明可提供有价值的极端同酶，该酶适应在极端环境条件下催化反应。 一种众所周知的极端同酶是来自高疗热水素的TAQ聚合酶。 这项研究的重点是内布拉斯加州西部研究的小盐，碱性湖泊。 我们将隔离并确定能够为众多生物技术应用产生兴趣极端的潜在微生物。 我们将寻找活性在高pH的活性的酯酶，木聚糖酶，纤维素酶，蛋白酶和脂肪酶。 将分离能够产生这些酶的微生物，表征的酶活性，纯化酶以及鉴定并克隆到大肠杆菌中的酶基因序列。