ENHANCEMENT OF DENGUE VIRUS TRANSMISSION DUE TO SALIVARY PROTEINS OF ITS VECTOR

由于其载体的唾液蛋白而增强登革热病毒的传播

• 批准号: 8167889
• 负责人: Christopher Mores
• 金额: $ 7.38万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167889
• 关键词: Aedes; Affect; Antibodies; Arbovirus Infections; Binding; Bite; Complex; Computer Retrieval of Information on Scientific Projects Database; Culicidae; Dengue; Dengue Virus; Ecology; Funding; Grant; Immune response; Infection; Institution; Life; Proteins; Research; Research Personnel; Resources; Salivary Glands; Salivary Proteins; Serotyping; Source; United States National Institutes of Health; base; protein expression; tool; transmission process; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Section 1. Dengue-induced differential protein expression in salivary gland extracts of Aedes aegypti mosquitoes Based on the observed differences in protein expression between infected and uninfected mosquito bodies, we decided to evaluate if this phenomenon was also observed in their salivary glands. Infection of salivary glands by dengue virus is a required step in dengue fever transmission. Section 2. Interaction of Dengue virus with salivary gland proteins Recently, several salivary gland proteins from Aedes mosquitoes were found to bind to dengue virus (Cao-Lormeau et al. 2009). However, it was not clear whether these proteins were structural or secreted. We plan to study salivary gland proteins that are able to bind any of the four serotypes of dengue virus. Section 3. Humoral immune response against Ae. aegypti SGE Salivary gland proteins have shown to be a very useful tool for evaluating mosquito bite exposure. We wanted to evaluate the anti  Ae. aegypti SGE antibody level in people living in dengue endemic regions. Section 4. Transmission dynamics of complex dengue virus ecologies Globally, dengue virus is the most significant arboviral infection, affecting millions of people throughout the tropics. We are investigating the transmission dynamics of dengue virus in the context of multiple, co-circulating serotypes and strains.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 第1节。登革热诱导的差异蛋白表达在埃及埃及蚊子的唾液腺提取物中 根据感染和未感染的蚊子体之间观察到的蛋白质表达差异，我们决定评估是否在其唾液腺中也观察到这种现象。登革热病毒感染唾液腺是登革热传播所必需的一步。 第2节。登革热病毒与唾液腺蛋白的相互作用 最近，发现来自埃德斯蚊子的几种唾液腺蛋白与登革热病毒结合（Cao-Lormeau等，2009）。但是，尚不清楚这些蛋白是结构性的还是分泌的。我们计划研究能够结合登革热病毒的四种血清型中任何一种的唾液腺蛋白。 第3节。针对AE的体液免疫反应。埃及SGE 唾液腺蛋白已显示是评估蚊子咬合暴露的非常有用的工具。我们想评估抗AE。居住在登革热地区的人们的埃及SGE抗体水平。 第4节。复杂登革热病毒生态的传播动力学 在全球范围内，登革热病毒是最重要的丁香病毒感染，影响了整个热带地区数百万的人。我们正在研究登革热病毒的传播动力学，在多种共同循环的血清型和菌株的背景下。