CORE D: DYNAMIC IMAGING FACILITY

核心 D：动态成像设施

• 批准号: 8168466
• 负责人: Terence Keith Smith
• 金额: $ 16.86万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168466
• 关键词: Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Data Files; Electrophysiology (science); Equipment; Funding; Grant; Housing; Image; Image Analysis; Institution; Maps; Motion; Movement; Physiological; Recommendation; Research; Research Personnel; Resources; Services; Source; Temperature; Tissues; Training; United States National Institutes of Health; Video Recording; movie; programs; reconstruction; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core D- Dynamic imaging: provides dynamic imaging and analysis services tothe COBRE program. CORE D provides equipment and expertise to carry outCa2+/video imaging experiments and assists in the analysis of any type ofimaging data. CORE D has 2 dedicated Ca2+ imaging rooms, each with acomplete setup for acquiring Ca2+ movies combined with either electrophysiology and/or tension recordings. Experiments can be performed on single cells or in whole tissues at room or physiological temperatures. In addition, other setups allow video recordings of movements in intact or dissected tissues. In order to analyze data in an efficient manner, CORE D has a central file server and RAID storage pool for long term storage and quick access to data files. An important component of CORE D is the ability to tailor analysis of data to answer specific questions posed by the PIS. An in-house analysis program (Volumetry G6a) is available that is used for all analysis including: motion stabilization and analysis, spatio-temporal map analysis, coordination/correlation of activities and 3D reconstruction. CORE D provides each user with extensive image analysis training and recommendations for optimizing experiments.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 Core D-动态成像：为COBRE程序提供动态成像和分析服务。 CORE D 提供进行 Ca2+/视频成像实验的设备和专业知识，并协助分析任何类型的成像数据。 CORE D 拥有 2 个专用 Ca2+ 成像室，每个房间都有完整的设置，用于采集 Ca2+ 电影并结合电生理学和/或张力记录。实验可以在 在室温或生理温度下的单个细胞或整个组织中。此外，其他设置允许对完整或解剖组织的运动进行视频记录。为了高效地分析数据，CORE D 有一个中央文件服务器 和 RAID 存储池，用于长期存储和快速访问数据文件。 CORE D 的一个重要组成部分是能够定制数据分析以回答 PIS 提出的特定问题。内部分析程序（体积测量 G6a）可用于所有分析，包括：运动稳定和 分析、时空地图分析、活动协调/关联和 3D 重建。 CORE D 为每个用户提供广泛的图像分析培训和优化实验的建议。