TRANSCRIPTIONAL REGULATION OF A FUMIGATUS VIRULENCE

烟菌毒力的转录调控

• 批准号: 8174490
• 负责人: Scott G Filler
• 金额: $ 0.41万
• 依托单位: LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8174490
• 关键词: A549; American Type Culture Collection; Aspergillus fumigatus; Blood Vessels; Cell Line; Cells; Computer Retrieval of Information on Scientific Projects Database; Endothelial Cells; Funding; Goals; Grant; Harvest; Human; Infection; Institution; Invaded; Link; Malignant neoplasm of lung; Medical center; Methods; Mothers; Patients; Process; Research; Research Personnel; Research Project Grants; Resources; Source; Transcriptional Regulation; Umbilical Cord Blood; Umbilical cord structure; United States National Institutes of Health; Vaginal delivery procedure; Veins; Virulence; Work; cell injury; fungus; mortality; pneumocyte; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this research project is study the interactions of the fungus, Aspergillus fumigatus, with endothelial cells. These cells form the inner lining of the blood vessels. The long-range goal of this research is to understand how A. fumigatus invades, stimulates, and damages these cells so that methods to block this processes can be developed. This work is important because the mortality of patients with a disseminated candidal infection is approximately 50%, even with currently available therapy. For the proposed studies, the endothelial cells will be harvested from the veins within human umbilical cords and grown in tissue culture. The umbilical cords will be obtained after normal vaginal births from uninfected donors. No information about the mother or baby will be collected. The donors of the cords will not be identified and the umbilical cord blood will not be collected. The umbilical cords will collected at both Harbor-UCLA Medical Center and at Cedars-Sinai Medical Center. This study will also study the interactions of A. fumigatus with the A549 type 2 pneumocyte cell line. This cell line was originally isolated from a human with lung cancer in 1972. It will be obtained from the American Type Culture Collection. There is no information linking the identity of the this patient with the cell line.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该研究项目的目的是研究真菌，曲霉曲霉与内皮细胞的相互作用。 这些细胞形成了血管内壁。 这项研究的远程目标是了解烟曲霉如何侵入，刺激和损害这些细胞，以便可以开发阻断此过程的方法。 这项工作很重要，因为即使有目前可用的治疗，患有候选候选感染的患者的死亡率也约为50％。 对于拟议的研究，内皮细胞将从人脐带中的静脉收获，并在组织培养中生长。 未感染的供体正常阴道分娩后，将获得脐带。 不会收集有关母亲或婴儿的信息。 将无法确定绳索的供体，并且不会收集脐带血。 脐带将在Harbour-Ucla医疗中心和Cedars-Sinai医疗中心收集。 这项研究还将研究烟曲霉与A549 2型肺细胞细胞系的相互作用。 该细胞系最初是从1972年从肺癌的人类中分离出来的。它将从美国型培养物中获得。 没有将该患者与细胞系的身份联系起来的信息。