DNA ANALYSIS CORE FACILITY

DNA 分析核心设施

基本信息

批准号：
8357081
负责人：
KYLE L JOHNSON
金额：
$ 16.76万
依托单位：
UNIVERSITY OF TEXAS EL PASO
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2012-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8357081
关键词：
Biomedical Research Blood capillaries Core Facility Custom DNA DNA Microarray Chip DNA Sequence DNA Sequence Analysis DNA analysis Data Analyses Data Collection Documentation Equipment Fees Floor Funding Gel Genetic Grant Human Resources Laboratories Mexico National Center for Research Resources Principal Investigator Printing Process Protein Microchips Protocols documentation RNA Reaction Research Research Infrastructure Research Personnel Resources Reverse Transcription Scanning Services Slide Source System Technical Expertise Techniques Texas Training United States National Institutes of Health Update capillary cost design genome sequencing

Biomedical Research Blood capillaries Core Facility Custom DNA DNA Microarray Chip DNA Sequence DNA Sequence Analysis DNA analysis Data Analyses Data Collection Documentation Equipment Fees Floor Funding Gel Genetic Grant Human Resources Laboratories Mexico National Center for Research Resources Principal Investigator Printing Process Protein Microchips Protocols documentation RNA Reaction Research Research Infrastructure Research Personnel Resources Reverse Transcription Scanning Services Slide Source System Technical Expertise Techniques Texas Training United States National Institutes of Health Update capillary cost design genome sequencing

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The analysis of DNA and RNA is an essential aspect of cutting-edge biomedical research. In order to facilitate the application of state-of-the-art techniques to research problems relevant to the Texas/Mexico border region, this proposal outlines new initiatives for the DNA Analysis Core Facility. The updated Core combines existing equipment for quantitative reverse-transcription PCR (qRT-PCR), data collection and analysis (UV/VIS spectrophotometers, gel documentation system, and storage PhorphorImager), and custom microarray printing, hybridization and scanning equipment with a newly-acquired high capacity capillary DNA sequencer. The ability to perform high-throughput genome sequencing will augment existing technical strengths in microarray design and processing. Implementation of a sliding fee scale for DNA sequencing and microarray services provided to BBRC researchers, UTEP investigators outside the BBRC, and to regional and national researchers outside of UTEP will allow the DNA Analysis Core Facility to transition to a self-supporting status. The relocation of the Core to new, greatly expanded laboratory space on the 3rd floor of the Bioscience Research Building, together with the addition of new technical expertise, will permit the DNA Analysis Core to keep pace with the rate of demand placed upon it by an increasing number of biomedical researchers. We propose the following Specific Aims: 1. Respond to increasing microarry use by assisting researchers in developing protocols and optimizing reaction conditions for DNA and protein microarrays 2. Develop protocols for DNA sequence analysis using the newly acquired Genetic Analyzer and train appropriate personnel in its use 3. Transform the Core into a self-sustainable facility that provides the region with rapid, top-quality DNA sequencing and microarray services

该副本是利用资源的众多研究子项目之一由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持而且，副投影的主要研究员可能是其他来源提供的包括其他NIH来源。列出的总费用可能代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 DNA和RNA的分析是尖端生物医学研究的重要方面。为了促进最先进的技术在研究与德克萨斯/墨西哥边境地区有关的问题上，该提案概述了DNA分析核心设施的新计划。更新的核心结合了现有的定量反向转录PCR（QRT-PCR），数据收集和分析（UV/VIS分光光度计，凝胶文档系统和储存反形的设备）以及定制的微阵列打印，杂交和扫描设备，并具有新的高能量高能量capillage sequillary sequillary sequillary sequillary sequillary DNA。进行高通量基因组测序的能力将增强微阵列设计和处理中的现有技术优势。实施向BBRC研究人员，BBRC外的UTEP研究人员以及UTEP以外的区域和国家研究人员提供的DNA测序和微阵列服务的滑动费量表，将使DNA分析核心设施能够过渡到自支持状态。将核心搬迁到生物科学研究大楼三楼的新的，大大扩展的实验室空间，再加上新的技术专长，将使DNA分析核心与越来越多的生物医学研究人员保持同步的速度。我们提出以下具体目标： 1。通过协助研究人员制定方案并优化DNA和蛋白质微阵列的反应条件来应对增加的微单和使用。 2。使用新获得的遗传分析仪制定DNA序列分析的协议，并在其使用中培训适当的人员 3。将核心转变为可自我维持的设施，为该地区提供快速，顶级的DNA测序和微阵列服务