ANATOMICAL AND PHYSIOLOGICAL CHARACTERIZATION OF THE THALAMOSTRIATAL SYSTEM

丘纹系统的解剖学和生理学特征

• 批准号: 8357545
• 负责人: Yoland Smith
• 金额: $ 3.29万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357545
• 关键词: Basal Ganglia; Cells; Corpus striatum structure; Deep Brain Stimulation; Electrons; Functional disorder; Funding; Grant; Immunohistochemistry; Injection of therapeutic agent; Interneurons; Label; Mediating; Microscopic; Monkeys; Motor; National Center for Research Resources; Neurons; Parafascicular Nucleus; Parkinson Disease; Pharmaceutical Preparations; Physiological; Play; Positioning Attribute; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Role; Source; Support System; Symptoms; Synapses; System; Thalamic structure; Therapeutic Effect; United States National Institutes of Health; cholinergic; cost; nonhuman primate; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The interaction between the thalamus and the striatum has been implicated in disturbances of basal ganglia function such as Parkinson's disease. Deep brain stimulation of the centromedian/parafascicular (CM/PF) thalamic nuclei is effective in relieving motor symptoms associated with these conditions; however, its therapeutic effects are hampered by the minimal information known about the function of the thalamostriatal system. This study aims to examine the CM/PF-striatal anatomical and functional relationship in nonhuman primates, in order to understand the involvement of the thalamostriatal system in basal ganglia dysfunction. Our findings show that cholinergic interneurons mainly respond to CM activation by decreased activity, which is mediated by the local injection of GABAergic drugs. In the anatomical studies, immunohistochemistry at the electron microscopic level was double-labeled with neuronal markers for medium spiny neurons (MSNs) and striatal cholinergic interneurons (SCIs), in order to determine what portion of the GABAergic inputs observed onto SCIs are derived from MSNs, the main cell group of the striatum. Our findings demonstrate that SCIs mainly receive symmetric synaptic inputs along their entire somatodendritic domain, where MSN collaterals comprise about one quarter of these synaptic inputs. In conclusion, intrastriatal GABAergic microcircuitry is shown to be in a position to play an important role in modulating the responses of SCIs to the activation of the CM in monkeys.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 丘脑和纹状体之间的相互作用与基底神经节功能（例如帕金森氏病）的干扰有关。 深脑刺激着脑膜/副脑（CM/PF）丘脑核可有效缓解与这些条件相关的运动症状。然而，关于丘脑纹状体系统功能的最低信息，它的治疗作用受到了阻碍。 这项研究旨在检查非人类灵长类动物的CM/PF - 纹状体解剖和功能关系，以了解丘脑纹状体系统在基底神经节功能障碍中的参与。 我们的发现表明，胆碱能中间神经元主要通过降低活性来响应CM激活，这是由局部注射GABA能药物介导的。 在解剖学研究中，电子显微镜水平上的免疫组织化学用中棘神经元（MSN）和纹状体胆碱能中间神经元（SCI）的神经元标记倍列，以确定SCIS上的GABA能输入中的哪些部分是从MSN中衍生出的。 我们的发现表明，SCI主要沿其整个体体域接收对称突触输入，其中MSN侧支约占这些突触输入的四分之一。 总之，纹状体内GABA能微电路循环被证明可以在调节SCIS对猴子中CM激活的响应方面发挥重要作用。