Bioassay Core

生物测定核心

基本信息

批准号：
8134163
负责人：
Yanbin Dong
金额：
$ 43.43万
依托单位：
AUGUSTA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8134163
关键词：
Albumins Aldosterone Angiotensin II Antioxidants Biochemical Biochemistry Biological Assay Corticosterone Corticotropin Creatinine DNA Data Data Storage and Retrieval Databases Electrodes Electrolytes Ensure Enzyme-Linked Immunosorbent Assay Epinephrine Equipment Genes Genetic Genetic Polymorphism Genetic Services Genetic Techniques Genome Genotype Glucose High Density Lipoprotein Cholesterol High Pressure Liquid Chromatography Human Hydrocortisone Hydrogen Peroxide Individual Insulin Knockout Mice Laboratories Leptin Mediation Methods Molecular Genetics Nitrates Nitrites Norepinephrine Plasma Price Procedures Quality Control Radioimmunoassay Rattus Receptor Gene Receptor, Angiotensin, Type 1 Records Renin Research Personnel Resource Development Role Sampling Schedule Services Single Nucleotide Polymorphism Tail Techniques Tissue Extracts Transforming Growth Factor beta Transgenic Organisms Urine Validation Validity and Reliability Wild Type Mouse Work data management design experience human CYBA protein isoprostaglandin F2alpha type-III low density lipoprotein triglyceride post gamma-globulins programs receptor skills statistics tetrahydrobiopterin

项目摘要

The Bioassay Core will provide biochemical assay and genotyping services for the individual projects within the Program Project. Biochemical assays that will be performed include analyses in plasma and urine for human and rat samples. The following assays will be performed: Plasma renin activity, aldosterone, angiotensin II, cortisol, corticosterone, ACTH, nitrite, nitrate, 8-isoprostane, TEARS, antioxidant capacity, hydrogen peroxide, norepinephrine, epinephrine, albumin, electrolytes, creatinine, total cholesterol, HDL, LDL, triglycerides, glucose, insulin, cystatin C, tetrahydrobiopterin, leptin, and transforming growth factor beta. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, ELISA, colorimetric, and ionselective electrodes. Genotyping of tagging single nucleotide polymorphisms of the genes of GCCR, CRHR1, p22phox, and EC-SOD will be performed for Project 2. Genotyping of the C-521T polymorphism of the angiotensin II receptor type 1 gene will be performed for Project 1. Genotyping services will be also performed for Project 3 and 4 using the tail snip DMA of the transgenic rats of the ETB receptor deficient rat and endothelial ET knockout mice. The extracted DMA will be genotyped to determine whether the rats are wildtype, heterozygous, or homozygous for the ETB receptor gene and whether the mice are wild-type, heterozygous, or homozygous for the ET gene. The core will also provide resources for development of additional assays as needed by individual investigators. The core will assure assay quality control through validation procedures.

生物测定核心将为程序项目中的各个项目提供生化测定和基因分型服务。将进行的生化测定包括血浆和尿液和大鼠样品的分析。将进行以下测定：血浆肾素活性，醛固酮，血管紧张素II，皮质醇，皮质酮，皮质酮，ACTH，硝酸盐，硝酸盐，8-异丙烷，泪水，泪水，抗氧化能力，过氧化氢，无毒性，去甲肾上腺素，去甲肾上腺素，肾上腺素，蛋白，蛋白质蛋白，蛋白质蛋白质蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质，蛋白质。，HDL，LDL，甘油三酸酯，葡萄糖，胰岛素，半胱氨酸蛋白酶C，四氢无生蛋白，瘦素和转化生长因子β。技术将包括放射免疫测定，放射酶测定，HPLC，ELISA，比色和离子电极。将针对项目2进行标记的单核苷酸多态性的基因分型。还将使用ETB受体缺乏大鼠和内皮ET基因敲除小鼠的转基因大鼠的尾部剪接DMA为项目3和4进行服务。将对提取的DMA进行基因分型，以确定ETB受体基因的大鼠是野生型，杂合或纯合子，以及小鼠是野生型，杂合子还是纯合子的ET基因。该核心还将为各个调查人员的需要提供资源，以开发其他测定法。核心将通过验证程序。