PROJECT 3- TOXICOGENOMICS ARISTOLOCHIC ACID NEPHROPATHY

项目 3 - 毒理学马兜铃酸肾病

基本信息

批准号：
8069937
负责人：
Arthur Patrick Grollman
金额：
$ 54.94万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-05 至 2012-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8069937
关键词：
Accounting Acute Address Adult Affect Archives Aristolochia Aristolochic Acids Balkan Nephropathy Biological Markers Blood Bread Candidate Disease Gene Characteristics Chronic Chronic Kidney Insufficiency Collaborations Control Groups Controlled Study Croatia DNA DNA Adducts DNA Repair Data Data Set Databases Development Disease Dose Enrollment Epidemiologic Studies Epidemiology Exposure to Fibrosis Flour Genes Genetic Genetic Polymorphism Genetic Predisposition to Disease Genomics Genotype Haplotypes Head Health Surveys High Pressure Liquid Chromatography Inbred Strains Mice Individual Ingestion Inherited Institutes Kidney Kidney Diseases Leukocytes Life Malignant Neoplasms Maps Medical Metabolic Biotransformation Modeling Mus National Institute of Environmental Health Sciences Nephrology Nephrotoxic Nucleotides Patients Pattern Persons Plants Poisoning Predisposition Public Health Quantitative Trait Loci Questionnaires Recommendation Relative (related person)Renal function Research Research Design Research Personnel Resistance Risk Rodent SNP genotyping Sampling Serbia Serum Specimen Testing Tissues Toxicogenomics Training Tumorigenicity Universities Urine Urothelial Cell Variant adduct aristolochic acid I aristolochic acid II base case control cost genetic linkage meetings mouse model nephrotoxicity professor programs repaired tumor

项目摘要

to more than 1,000 adults living in these villages during the spring of 2005. Blood and urine samples were collected from each subject and a detailed questionnaire was administered. These data have now been entered into a database by trained local professionals who have made extensive efforts to check and/or verify information when missing values and suspicious extreme values were observed, returning to the villages to obtain these important data. As a result, missing values have been kept to a minimum and the initial data set, which will be used in the toxicogenomics study, is expected to be ready for formal analysis early in 2007. Serum samples obtained from 1,000 subjects are stored at the Institute of Public Health in Slavonski Brod, Croatia, and the analyses reflecting renal function of these persons are now largely complete. Leukocyte DNAs were extracted by Professor Branko Brdar, a Stony Brook-trained Croatian medical researcher, and his associates. Based on random screens, we have determined that the quality and quantity of DNA extracted from those samples is excellent, permitting us to proceed with the proposed SNP genomic analyses. Our proposal for support to the Center for Inherited Disease Research (CIDR), a centralized facility for SNP genotyping, has been approved by NIEHS. The CIDR expert review panel has reviewed the information contained in Aims 1 & 2 as they relate to genotyping analyses. Their recommendation to assign CIDR staff to participate in this project and to cover most of the costs of genotyping will be considered by the CIDR Board of Governors at their October meeting. Extending our ongoing collaboration with Prof. Xiaomei Li, Head of Nephrology at the University of Beijing, we analyzed 27 samples of exfoliated urothelial cells from patients with documented AAN who are being followed PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page 155 Continuation Format Page

在这些村庄中有1000多名成年人 2005年春季。从每个受试者那里收集血液和尿液样本，详细的问卷是管理。这些数据现在已被训练有素的本地专业人员输入当缺失值和可疑的极端值时，大力检查和/或验证信息观察到，返回村庄以获取这些重要数据。结果，缺少值是保持最低限度，预计将在毒物基因组学研究中使用的初始数据集将是准备在2007年初进行正式分析。从1,000名受试者获得的血清样本存储在斯拉文斯基Brod的公共卫生研究所，克罗地亚以及反映这些人的肾功能的分析现在已经完成了。白细胞DNA 由斯托尼布鲁克培训的克罗地亚医学研究员Branko Brdar教授及其同事。基于随机屏幕，我们确定了提取的DNA的质量和数量这些样品非常好，可以使我们继续进行拟议的SNP基因组分析。我们的支持SNP的集中设施的遗产疾病研究中心支持支持的建议基因分型已获得NIEHS的批准。 CIDR专家评论小组审查了信息与基因分析有关的目标1和2中包含。他们的建议将CIDR员工分配给参加该项目并涵盖大多数基因分型费用，将由CIDR委员会考虑州长在十月的会议上。扩展我们与北京大学肾脏学系主任李小龙李教授的持续合作，我们分析了27种来自记录的AAN患者的去角质尿路上皮细胞样本 PHS 398/2590（Rev. 09/04，重新发行4/2006）Page 155延续格式页面