GENOMICS

基因组学

• 批准号: 8182222
• 负责人: Agnes Viale
• 金额: $ 76.64万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8182222
• 关键词: Administrator; Bioinformatics; Cancer Center Support Grant; Computer software; Consultations; Core Facility; Custom; DNA; Data; Data Analyses; Data Set; Databases; Ensure; Experimental Designs; Gene Expression Profiling; Genetic Transcription; Genomics; Growth; Head; Human Resources; Hybridization Array; Image Analysis; Institutes; Label; Memorial Sloan-Kettering Cancer Center; Nucleic Acids; Polymorphism Analysis; Preparation; Procedures; Process; Production; Quality Control; Research; Research Personnel; Sampling; Services; Single Nucleotide Polymorphism; Spottings; Time; Training; Validation; comparative genomic hybridization; operation; research study

The Genomics Core was established in January 2001 and provides consultation, training and technical assistance for the principal components of a gene expression profiling experiment, analysis of genomic DMA and quantitative PCR. The facility personnel consists of Dr.Agnes Viale, core head, six research assistants, a database administrator/bioinformatics expert, and an administrative assistant. This core supports the Aftymetrix, Illumina, Agilent and custom spotted array platforms. The services offered are ranging from nucleic acid extraction to preliminary data analysis. For transcription profiling experiments, comparative genomic hybridization (CGH) and Single Nucleotide Polymorphism (SNP) analysis, investigators are assisted during experimental design, sample preparation and labeling, hybridization, array post processing, image analysis. A weekly training for specific software (GCOS, Genespring, CGH-analytics) is also opened to the users of the core. The core's servers and workstations are connected to the MSKCC network, allowing investigators to access their data remotely. Integrity of the database is under the responsibility of full-time computational biologist/database administrator. The GC developed stringent preprocessing quality controls and instituted technical standard operation procedures to ensure that the GC generated homogeneous datasets of extremely high quality. Since its inception, the GC has enjoyed an exponential growth in the number of users and accordingly, in the number of samples that were processed.

基因组核心成立于2001年1月，提供咨询，培训和技术 基因表达分析实验的主要成分的帮助，基因组DMA的分析 和定量PCR。该设施人员由Agnes Viale博士组成，核心负责人，六名研究助理， 数据库管理员/生物信息学专家和行政助理。这个核心支持 AftyMetrix，Illumina，Agilent和自定义斑点阵列平台。提供的服务范围从 核酸提取到初步数据分析。对于转录分析实验，比较 基因组杂交（CGH）和单核苷酸多态性（SNP）分析，研究者是 在实验设计，样品制备和标签，杂交，阵列后处理过程中得到的协助， 图像分析。还打开了针对特定软件的每周培训（GCO，GENESPRING，CGH-ANALYTICS） 给核心用户。核心的服务器和工作站连接到MSKCC网络， 允许研究人员远程访问数据。数据库的完整性是由 全日制计算生物学家/数据库管理员。 GC发展了严格的预处理质量 控制和建立的技术标准操作程序，以确保GC生成 质量极高的均匀数据集。自成立以来，GC一直享有指数 用户数量的增长，因此，处理的样本数量。