Commercialization of a Human Myocyte and Adipocyte Co-Culture System

人类心肌细胞和脂肪细胞共培养系统的商业化

• 批准号: 7611887
• 负责人: YOLANDA Renee LEA-CURRIE
• 金额: $ 70.23万
• 依托单位: ZEN-BIO, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-15 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7611887
• 关键词: Adipocytes; Adipose tissue; Advisory Committees; Biological Assay; Blood; Calcium; Cells; Coculture Techniques; Communities; Complex; Contract Services; Contracts; Cultured Cells; Defect; Diabetes Mellitus; Disease; Health; Health Services Research; Housing; Human; In Vitro; Insulin; Insulin Resistance; Investigation; Link; Maintenance; Mechanics; Metabolic; Metabolic Diseases; Methodology; Methods; Molecular; Muscle; Muscle Cells; Muscle Fibers; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Patients; Pattern; Peripheral; Personal Satisfaction; Phase; Physiological; Population; Protocols documentation; Public Health; Purpose; Quality Control; Regulation; Research; Research Contracts; Research Personnel; Services; Shipping; Ships; Signal Transduction; Site; Skeletal Muscle; Skeletal system; Stimulus; Strategic Planning; Stretching; System; Targeted Research; Techniques; Testing; Tissue Procurements; Tissues; United States National Institutes of Health; Validation; abdominal fat; adipocyte biology; base; blood glucose regulation; cell type; commercialization; cost; demographics; experience; fatty acid oxidation; glucose disposal; inhibitor/antagonist; insulin sensitivity; novel strategies; prospective; repository; satellite cell; subcutaneous; tool

DESCRIPTION (provided by applicant): The dramatic rise in obesity within the U.S. over the past two decades has led to an increase in Type 2 diabetes and related health disorders. The National Institutes of Health has responded by establishing a task force devoted to accelerating the progress in obesity research. In August of 2004 the task force established a strategic plan for obesity research including research targeted at breaking the link between obesity and its related health disorders. Increased abdominal adiposity is correlated with a defect in glucose homeostasis and associated with decreased insulin sensitivity of peripheral tissue. Skeletal muscle is an important site of insulin-stimulated glucose disposal and often the site of insulin resistance in obesity. The molecular mechanisms of skeletal muscle insulin resistance remain to be uncovered and this gap is the basis of this proposal. We have established a human skeletal muscle cell system that responds to physiologic stimuli in vitro. Additionally, we have established and validated an adipocyte / myocyte co-culture system to investigate the interplay between these two cell types. Our preliminary studies show that adipocytes have a profound effect on skeletal myocyte metabolic activities. Based on the successful completion of Phase I, we are eager to extend our research in Phase II by focusing on making the co-culture system commercially available. We will continue the optimization and characterization of two skeletal muscle cell systems using the following aims. Aim 1 will continue the optimization and characterization of isolated human skeletal muscle cells, establish quality control criteria, and validate contract research services using this system. Aim 2 is focused on establishing and validating the adipocyte/myocyte co-culture system for commercial availability. We will investigate the effects of donor demographics on each cell type in the co-culture, establish quality control criteria, and identify methods for shipping and customer co-culture maintenance. Aim 3 is to establish a repository of donor-matched skeletal muscle, subcutaneous, and omental adipose tissue from patients of different demographics. These tissues and derived cells will be made available to researchers in the obesity and diabetes research fields. PUBLIC HEALTH RELEVANCE: At the completion of this project, a commercially available, fully validated human skeletal myocyte system, skeletal myocyte / adipocyte co-culture system, and related assay kits will be offered to researchers. The availability of these systems will provide opportunities for new approaches in the investigation of metabolic disease and a unique methodology to examine the complex interaction between these two cell types.

描述（由申请人提供）：过去 20 年来，美国肥胖率急剧上升，导致 2 型糖尿病和相关健康疾病的增加。美国国立卫生研究院对此做出了回应，成立了一个工作组，致力于加速肥胖研究的进展。 2004 年 8 月，工作组制定了一项肥胖研究战略计划，其中包括旨在打破肥胖与其相关健康疾病之间联系的研究。腹部肥胖增加与葡萄糖稳态缺陷相关，并与外周组织胰岛素敏感性降低相关。骨骼肌是胰岛素刺激的葡萄糖处理的重要部位，并且通常是肥胖症中胰岛素抵抗的部位。骨骼肌胰岛素抵抗的分子机制仍有待揭示，这一差距是该提议的基础。我们建立了一种能够在体外对生理刺激做出反应的人类骨骼肌细胞系统。此外，我们还建立并验证了脂肪细胞/肌细胞共培养系统，以研究这两种细胞类型之间的相互作用。我们的初步研究表明脂肪细胞对骨骼肌细胞代谢活动具有深远的影响。在第一阶段成功完成的基础上，我们渴望通过专注于将共培养系统商业化来扩展第二阶段的研究。我们将按照以下目标继续优化和表征两个骨骼肌细胞系统。目标 1 将继续对分离的人类骨骼肌细胞进行优化和表征，建立质量控制标准，并使用该系统验证合同研究服务。目标 2 的重点是建立和验证脂肪细胞/肌细胞共培养系统的商业可用性。我们将研究供体人口统计数据对共培养中每种细胞类型的影响，建立质量控制标准，并确定运输和客户共培养维护的方法。目标 3 是建立来自不同人口统计患者的供体匹配骨骼肌、皮下和网膜脂肪组织的存储库。这些组织和衍生细胞将提供给肥胖和糖尿病研究领域的研究人员。公共健康相关性：该项目完成后，将向研究人员提供市售的、经过充分验证的人类骨骼肌细胞系统、骨骼肌细胞/脂肪细胞共培养系统以及相关检测试剂盒。这些系统的可用性将为代谢疾病研究的新方法和检查这两种细胞类型之间复杂相互作用的独特方法提供机会。