EPSTEIN-BARR VIRUS IN HODGKIN'S DISEASE

霍奇金病中的 Epstein-Barr 病毒

• 批准号: 2104979
• 负责人: SALLY L GLASER
• 金额: $ 5.71万
• 依托单位: CANCER PREVENTION INSTIT OF CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2104979
• 关键词: Epstein Barr virus; Hodgkin's disease; cancer risk; female; histology; hormones; human age group; human subject; in situ hybridization; interview; latent virus infection; lifestyle; neoplasm /cancer epidemiology; polymerase chain reaction; racial /ethnic difference; social class; statistics /biometry

This Small Grant project will expand an ongoing, NCI-funded, epidemiologic case-control study of Hodgkin's disease (HD) in San Francisco-Bay Area women by collecting and incorporating data on Epstein-Barr virus (EBV) in tumor cells of case subjects. EBV recently has been implicated as a pathogenetic cofactor in about one-half of HD tumors. As HD thus may be subclassifiable as EBV-positive and EBV-negative, addition of EBV data to the ongoing study will enhance its primary investigation of reproductive risk factors by providing more precise disease endpoints, i.e., HD subgroups defined by EBV status. The aims of this Small Grant project are: 1) to collect archived tumor specimens from case subjects in the ongoing study, apply standard molecular virologic techniques to identify EBV latent gene products in HD tumor cells, and classify each case subject as EBV-positive or EBV-negative; 2) to determine if reproductive risks, e.g., a protective effect of parity, differ for women with EBV-positive and EBV- negative HD; 3) to describe the distribution of EBV-positive and EBV- negative HD by age, race, and histologic subtype, and explore risks for the two virus-defined subgroups associated with various additional factors being measured, including social class characteristics that increase HD risk. The ongoing study ultimately will involve 315 women with HD and 315 population controls in in-person interviews covering demographic, reproductive, hormone-use, social class, familial, medical, and other lifestyle topics. Diagnosis and histologic classification of HD are being reviewed for accuracy. To date, 96% of invited case subjects have been interviewed. For the Small Grant project, we will request interviewed subjects' tumor tissue specimens from pathologists; in a pilot study, we have acquired specimens for about 85% of subjects. Access to tumor specimens is time- limited, as it is based on signed consent from subjects to release pathologic materials for the ongoing study, soon to be completed. Specimens will be mailed to our coinvestigator, who will test sections for EBV latent gene products using polymerase chain reaction, EBER in situ hybridization, and immunoperoxidase techniques to detect LMP-1, with positive and negative controls. This hybridization method is sensitive, consistent, rapid, and produces no cellular or other herpesvirus cross- hybridization. Data analysis will characterize EBV-positive and EBV- negative HD by variables presently under investigation, using descriptive statistics. Multivariate statistical methods will estimate and compare odds ratios for EBV-positive and EBV-negative HD associated with reproductive and other risk factors, after adjustment for suspected confounders. As one of the first studies to combine molecular virologic and extensive personal risk factor data in a population-based series of HD cases, this Small Grant project will provide initial epidemiologic evaluation of EBV-positive and EBV-negative HD by a variety of characteristics postulated and established to increase risk of this lymphoma.

这个小型赠款项目将扩大持续的，NCI资助的流行病学 旧金山 - 拜伊地区霍奇金氏病（HD）的病例对照研究 通过收集和合并有关爱泼斯坦 - 巴尔病毒（EBV）的数据中的妇女 病例受试者的肿瘤细胞。 EBV最近被认为是 大约一半的HD肿瘤中的致病辅助因子。因此，高清可能是 可作为EBV阳性和EBV阴性的子分类，将EBV数据添加到 正在进行的研究将增强其对生殖的主要研究 通过提供更精确的疾病终点（即高清）来风险因素 EBV状态定义的子组。这个小型赠款项目的目的是： 1）从正在进行中的病例受试者那里收集存档的肿瘤标本 研究，应用标准分子病毒学技术来识别EBV HD肿瘤细胞中的潜在基因产物，并将每个病例对象分类为 EBV阳性或EBV阴性； 2）确定生殖风险是否存在，例如 均等的保护作用，EBV阳性和EBV的女性有所不同 负HD； 3）描述EBV阳性和EBV的分布 按年龄，种族和组织学亚型按年龄，种族和组织学负数负高清，并探索风险 与各种其他因素相关的两个病毒定义的亚组 被测量，包括增加高清的社会阶层特征 风险。正在进行的研究最终将涉及315名高清女性和315名女性 人口控制涉及人口统计的面对面访谈， 生殖，激素使用，社会阶层，家族，医学和其他 生活方式主题。 HD的诊断和组织学分类正在 审查准确性。迄今为止，有96％的受邀案件受试者是 采访。 对于小型赠款项目，我们将要求采访受试者的肿瘤 病理学家的组织标本；在一项试点研究中，我们获得了 大约85％受试者的标本。进入肿瘤标本是时间 - 有限，因为它基于受试者的签署同意 正在进行的研究的病理材料，即将完成。 标本将被邮寄给我们的共依申仪，后者将测试部分 EBV潜在基因产物使用聚合酶链反应，原位Eber 杂交和免疫过氧化物酶技术可检测LMP-1，与 阳性和负面对照。这种杂交方法是敏感的， 一致，快速，并且不会产生任何细胞或其他疱疹病毒交叉病毒 杂交。数据分析将表征EBV阳性和EBV- 当前正在研究的变量负HD，使用描述性 统计数据。多元统计方法将估算和比较 与EBV阳性和EBV阴性高清相关的赔率比 调整可疑的生殖和其他危险因素 混淆者。 作为结合分子病毒学的最早研究之一 以及基于人群的高清系列中广泛的个人风险因素数据 案例，这个小型赠款项目将提供初始的流行病学 通过各种EBV阳性和EBV阴性高清评估 假设并建立了以增加风险的特征 淋巴瘤。