Differentiation of Bone-Marrow Stem Cells in a Laser Patterned Myocyte Coculture

激光图案化肌细胞共培养中骨髓干细胞的分化

基本信息

批准号：
8074968
负责人：
BRUCE Z GAO
金额：
$ 14.3万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2012-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8074968
关键词：
Accounting Action Potentials Address Adult Americas Atomic Force Microscopy Biomedical Engineering Bone Marrow Bone Marrow Stem Cell Cardiac Cardiac Myocytes Cause of Death Cell Communication Cell Culture Techniques Cells Cellular biology Cessation of life Clinical Coculture Techniques Connexin 43 Core Facility Coupling Data Deposition Development Electrodes Embryo Engineering Environment Equilibrium Equipment Extracellular Matrix Figs - dietary Funding Goals Grant Heart Heart Diseases In Vitro Institution Island Lasers Manuscripts Measures Mechanics Microelectrodes Microscopy Modeling Molecular and Cellular Biology Monitor Mus Muscle Cells Myocardial Infarction Myocardium N-Cadherin Neonatal Optics Patch-Clamp Techniques Patients Pattern Physics Proliferating Property Regenerative Medicine Regulation Research Research Personnel Role Staging Stem cell transplant Stem cells Stress Structure System Testing Therapeutic Time Tissues Traction Training United States National Institutes of Health Woman Writing career cell type chemical property electrical property feeding foot functional restoration genetically modified cells improved in vivo injured men nanoscale patch clamp programs protein expression repaired skills training stem cell differentiation vector

项目摘要

DESCRIPTION (provided by applicant): This proposal describes training in cardiac-cell biology for a PI whose background is in physics and engineering. The training includes 1) graduate courses in cell biology; 2) experimental-skill training in molecular and cellular biology; and 3) academic development, including developing a research network and writing manuscripts/proposals. The immediate goal is to improve the Pi's ability to obtain NIH funding, particularly an R01 grant. The long-term goal is to assist the PI in developing his academic career in biomedical engineering with a better balance between his biomedical and engineering proficiencies. To achieve these goals, the PI proposes to investigate the functional differentiation of bone-marrow stem cells in an engineered cardiac myocyte-coculture model. In this model, identical cell-culture microwells will be fabricated to allow creation of a defined stem cell-myocyte interface in each well through application of a laser beam. This model will provide statistically significant data on the regulation of stem-cell differentiation by various types of cocultured myocytes. To test the hypothesis that the microenvironments will regulate the functional differentiation of stem cells into myocytes, the following specific aims will be addressed: 1) Determine the microenvironment's role in regulating stem-cell differentiation into cardiac cells by changing the extracellular matrix, switching the coculture cell types, and genetically modifying the cell-cell juctional protein expressions; 2) Use atomic force microscopy to determine the stem cells' mechanical properties by measuring cell stiffness and traction force microscopy to evaluate the cells' mechanical coupling; 3) Determine the stem cells' electrical properties by studying action potentials using the patch clamp technique and evaluating electrical coupling using microelectrode arrays. Heart disease is America's leading cause of death for both men and women, accounting for nearly 40% of all annual deaths. By adding to our knowledge of the functional differentiation of bone-marrow stem cells into heart muscle cells, the Pi's research will contibute to the current effort in regenerative medicine to restore the the function of a damaged heart using the patient's own cells. The Pi's institution will provide more than 2,500 sq feet of lab space and unlimited access to all core facilities, which are staffed and provided with state-of-the-art equipment, in addition to 75% release time.

描述（由申请人提供）：该提案描述了对物理和工程背景的PI的心脏细胞生物学培训。培训包括1）细胞生物学研究生课程； 2）分子和细胞生物学的实验技能培训； 3）学术发展，包括建立研究网络和编写手稿/建议。直接目标是提高PI获得NIH资金的能力，尤其是R01赠款。长期的目标是帮助PI发展生物医学工程领域的学术生涯，在他的生物医学和工程能力之间取得更好的平衡。为了实现这些目标，PI提议研究工程性心肌培养模型中骨髓干细胞的功能分化。在此模型中，将制造相同的细胞培养微孔，以通过应用激光束在每个井中创建定义的干细胞肌细胞界面。该模型将通过各种类型的共培养肌细胞提供有关干细胞分化调节的统计学意义数据。为了检验微环境将调节干细胞功能分化为心肌细胞的假设，将解决以下具体目的：1）确定微环境在调节干细胞中通过更换细胞外基质，切换cortulture，切换corculture，确定微环境在调节心脏细胞中的作用。细胞类型，并在遗传上修饰细胞 - 细胞蛋白表达； 2）使用原子力显微镜通过测量细胞刚度和牵引力显微镜来评估细胞的机械耦合来确定干细胞的机械性能； 3）通过使用斑块夹技术研究动作电位并使用微电极阵列评估电耦合来确定干细胞的电特性。心脏病是美国对男性和女性的主要死亡原因，占所有年死亡的近40％。通过增加我们对骨髓干细胞功能分化为心肌细胞的知识，PI的研究将与当前在再生医学上的努力相结合，以使用患者自身的细胞恢复受损心脏的功能。 PI的机构将提供超过2500平方英尺的实验室空间，并无限制地访问所有核心设施，这些核心设施配备人员并提供了最先进的设备，此外还有75％的发布时间。

项目成果

期刊论文数量（29）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Vulnerability to chosen-plaintext attack of optoelectronic information encryption with phase-shifting interferometry.

DOI：
10.1117/1.3590725
发表时间：
2011-06
期刊：
Optical engineering
影响因子：
1.3
作者：
W. Qin;Xiang Peng;Xiangfeng Meng;B. Gao
通讯作者：
W. Qin;Xiang Peng;Xiangfeng Meng;B. Gao

Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator.

DOI：
10.1364/ol.37.000827
发表时间：
2012-03-01
期刊：
Optics letters
影响因子：
3.6
作者：
Qin W;Shao Y;Liu H;Peng X;Niu H;Gao B
通讯作者：
Gao B

Addressable, large-field second harmonic generation microscopy based on 2D acousto-optical deflector and spatial light modulator.

基于二维声光偏转器和空间光调制器的可寻址大视场二次谐波发生显微镜

DOI：
10.1007/s00340-012-5164-9
发表时间：
2012-09-01
期刊：
APPLIED PHYSICS B-LASERS AND OPTICS
影响因子：
2.1
作者：
Shao, Yonghong;Liu, Honghai;Qin, Wan;Qu, Junle;Peng, Xiang;Niu, Hanben;Gao, Bruce Z.
通讯作者：
Gao, Bruce Z.

Depth of focus enhancement of a modified imaging quasi-fractal zone plate.

DOI：
10.1016/j.optlastec.2012.03.012
发表时间：
2012-10-01
期刊：
OPTICS AND LASER TECHNOLOGY
影响因子：
5
作者：
Zhang, Qinqin;Wang, Jingang;Wang, Mingwei;Bu, Jing;Zhu, Siwei;Gao, Bruce Z.;Yuan, Xiaocong
通讯作者：
Yuan, Xiaocong

Mesenchymal stem cell-cardiomyocyte interactions under defined contact modes on laser-patterned biochips.