Improving Biomaterials from a Cellular Point of View

从细胞的角度改进生物材料

基本信息

批准号：
7473161
负责人：
Venu Gopal Varanasi
金额：
$ 12.18万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2012-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7473161
关键词：
Affect Alkaline Phosphatase Aluminum Oxide Biocompatible Materials Bioglass 45S5 Body Fluids Bone Matrix Bone Regeneration Calcium Cell Communication Cell physiology Cells Chemicals Chemistry Condition Deposition Devices Differentiation Antigens Disease Extracellular Matrix Family Fostering Gene Expression Genes Glass Goals Growth Factor Hand Healed Hydroxyapatites Immersion Investigative Technique Immunoassay Immunohistochemistry Implant In Vitro Ions Laboratories Lead Life Liquid substance Methods Mission Mus Natural regeneration Object Attachment Osteoblasts Osteocalcin Osteogenesis Patients Physiological Play Polymerase Chain Reaction Polymers Process Property Purpose Reaction Research Research Personnel Role Silicon Structure Techniques Testing Thinking Time Tissues Titanium Transcription factor genes Transcriptional Activation United States National Institutes of Health Up-Regulation Work Wound Healing bone bone healing calcium phosphate concept controlled release desire extracellular healing implant material improved nano novel programs response stoichiometry success vapor

项目摘要

DESCRIPTION (provided by applicant): In this proposal, we will investigate a potential new way of bone healing. It is widely known that implant materials have been used for many years to support tissue repair or regeneration. It is now thought that such implants may also induce natural bone healing responses by living tissues and cells. Specifically, bioactive glasses have shown this ability to induce osteoblast response for bone healing by releasing bioactive glass ions. The purpose of this proposed work is to explore this novel idea that bioactive glass ions play an active role in bone healing and bone regeneration. To investigate the influence of these ions on bone healing, we will examine how these glasses interact with cells from an intracellular and extracellular perspective. First, it is believed that bioactive glass ions, which are release by glasses during immersion in physiological fluid, may induce various responses by osteoblasts. Second, these bioactive glass ions (namely silicon and calcium) may combinatorially control osteoblast function such that bone regeneration is hastened or enhanced. Finally, we investigate how bioactive glasses ion release can be controlled using a programmed bioactive glass. The techniques used to determine the influence of bioactive glass ions on gene expression and extracellular activity includes gene microarrays, real time polymer chain reaction, immunoassays, and immunohistochemistry. To program bioactive glasses, chemical vapor deposition is used to build nanolayered and microlayered composite glasses that deliver target ion concentrations to cells for enhanced osteoblast function. The aims and goals of this proposed work fit into the mission of NIH, that is, improving biomaterials by improving our understanding of cell-biomaterial interactions and exploring new methods in fabricating "smart" biomaterials. The work proposed here investigates biomedical device-cell interactions for bone healing. Specifically, we wish to control and improve bone healing through controlled biomaterial degradation in physiological fluid. The proposed work is aimed at developing materials that are self-regulating for gene-related therapies. Therefore, improving our understanding of tissue-biomedical device interactions can lead to devices that improve tissue healing and ease patient suffering owed to debilitating conditions and diseased.

描述（由申请人提供）：在本提案中，我们将研究一种潜在的骨愈合新方法。众所周知，植入材料多年来一直用于支持组织修复或再生。现在人们认为，这种植入物还可以诱导活组织和细胞的自然骨愈合反应。具体来说，生物活性玻璃已显示出通过释放生物活性玻璃离子诱导成骨细胞反应以促进骨愈合的能力。这项工作的目的是探索生物活性玻璃离子在骨愈合和骨再生中发挥积极作用的新想法。为了研究这些离子对骨愈合的影响，我们将从细胞内和细胞外的角度研究这些玻璃如何与细胞相互作用。首先，据信玻璃在浸入生理液体期间释放的生物活性玻璃离子可以诱导成骨细胞的各种反应。其次，这些生物活性玻璃离子（即硅和钙）可以组合控制成骨细胞功能，从而加速或增强骨再生。最后，我们研究了如何使用编程的生物活性玻璃来控制生物活性玻璃离子释放。用于确定生物活性玻璃离子对基因表达和细胞外活性影响的技术包括基因微阵列、实时聚合物链反应、免疫测定和免疫组织化学。为了对生物活性玻璃进行编程，化学气相沉积用于构建纳米层和微层复合玻璃，将目标离子浓度传递到细胞以增强成骨细胞功能。这项拟议工作的目的和目标符合 NIH 的使命，即通过提高我们对细胞-生物材料相互作用的理解并探索制造“智能”生物材料的新方法来改进生物材料。这里提出的工作研究了骨愈合的生物医学设备-细胞相互作用。具体来说，我们希望通过控制生理液中的生物材料降解来控制和改善骨愈合。拟议的工作旨在开发用于基因相关疗法的自我调节材料。因此，提高我们对组织-生物医学设备相互作用的理解可以带来改善组织愈合并减轻患者因衰弱状况和患病而造成的痛苦的设备。