DIET, INTESTINAL MICROFLORA AND COLORECTAL CANCER

饮食、肠道菌群和结直肠癌

• 批准号: 7376941
• 负责人: VOLKER MAI
• 金额: $ 0.79万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7376941
• 关键词: DIET; INTESTINAL; MICROFLORA; COLORECTAL; CANCER

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Central hypothesis: Diet defines and modulates the intestinal microflora, with both diet and intestinal microflora serving as modifiable risk factors for the occurrence of colon cancer. rationale: The majority (2/3 or more) of colon cancer cases appear to be associated with non-hereditary, environmental factors. However, few studies to date have incorporated comprehensive data on the contents of the human large bowel, despite the approximately 1011 bacteria per gram that constitute 55% of total stool solids. The microbial ecosystem is one of the most complex known, with several hundred species and subspecies. Studies conducted using traditional microbiologic techniques have suggested that patterns of fecal flora differ among groups from different geographic areas, who are consuming different types of diets, and who have different cancer risks. Additional studies suggest that there are a number of bacterial strains/forms in stool samples that are visible by microscopy but that do not grow on cultures i.e., they non-culturable using standard microbiologic media and techniques. The advent of molecular technology has provided us with tools that permit rapid, comprehensive analysis of bacterial communities including both the culturable and +non-culturable+ bacteria. Whereas DGGE allows for an efficient profiling of the intestinal microflora, 16S rDNA sequencing allows for both the semiquantitative enumeration of the most prevalent gut bacteria and the detection of hitherto unknown gut microbes. With such tools, we are in a position, for the first time, to look for linkages among diet, intestinal flora, and colon cancer. The proposed pilot study with 60 subjects is part of a larger study that will investigate these associations in a total of 500 subjects. The larger study is funded by an ACS mentored research scholar grant to the PI. The pilot study will allow us to investigate the proposed associations in more depth and optimize the analysis approach for the larger study. The results from the 60 subject pilot study will also form the basis for the resubmission of an ambitious PPG to the NCI and thus hopefully lead to additional extramural funding.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。中心假设：饮食定义和调节肠道微生物群，饮食和肠道微生物群都是结肠癌发生的可改变的危险因素。理由：大多数（2/3 或更多）结肠癌病例似乎与非遗传性环境因素有关。然而，迄今为止，很少有研究纳入有关人类大肠内容物的全面数据，尽管每克大约有 1011 个细菌，占粪便固体总量的 55%。微生物生态系统是已知最复杂的生态系统之一，有数百个物种和亚种。使用传统微生物技术进行的研究表明，来自不同地理区域、食用不同类型饮食以及具有不同癌症风险的人群的粪便菌群模式有所不同。其他研究表明，粪便样本中存在许多通过显微镜可见的细菌菌株/形式，但它们不能在培养物上生长，即它们无法使用标准微生物培养基和技术进行培养。分子技术的出现为我们提供了能够快速、全面分析细菌群落的工具，包括可培养和不可培养的细菌。 DGGE 可以对肠道菌群进行有效分析，而 16S rDNA 测序可以对最常见的肠道细菌进行半定量计数，并检测迄今未知的肠道微生物。有了这些工具，我们第一次能够寻找饮食、肠道菌群和结肠癌之间的联系。拟议的 60 名受试者试点研究是一项更大规模研究的一部分，该研究将在总共 500 名受试者中调查这些关联。这项更大规模的研究由 ACS 指导的研究学者向 PI 提供的资助资助。试点研究将使我们能够更深入地研究拟议的关联，并优化更大规模研究的分析方法。 60 个受试者试点研究的结果也将构成向 NCI 重新提交雄心勃勃的 PPG 的基础，从而有望获得额外的外部资金。