Human Intestinal Microbial Ecologies of Indigenous Communities

土著社区的人类肠道微生物生态学

基本信息

批准号：
8500365
负责人：
Paul Alan Lawson
金额：
$ 27.85万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8500365
关键词：
Address Altitude Americas Antibiotics Bacterial Genes Clinical Clostridium Communities Complex DNA Sequence Data Developed Countries Diet Diet Records Dietary Practices Disease Ecology Ecosystem Environment European Evaluation Evolution Feces Foundations Frequencies Genes Genome Geographic Locations Goals Grasslands Health Human Human Microbiome Indigenous Individual Intestines Investigation Knowledge Lead Life Location Medical Metabolism Methods Microbe Mummies North America Order Spirochaetales Outcome Peru Pharmaceutical Preparations Phylogenetic Analysis Population Process Published Comment Publishing Recording of previous events Recovery Research Role Sampling Sampling Studies Shapes South American Structure Taxon Technology Testing Treponema community living design disorder risk drug metabolism food resource functional genomics gene function genome sequencing gut microflora human disease lactic acid bacteria member microbial microbial genome microbiome next generation novel

项目摘要

DESCRIPTION (provided by applicant): The human gut is a complex ecosystem harboring up to a 1000 different microbial species. It is now known that microbial diversity within the gut impacts human health and metabolism and it is hoped that the study of human microbial diversity will lead to novel medical treatments. The objective of this study is to characterize gut microbes sampled from people living in remote geographic areas. This objective can have a transformative impact on human microbiome research, especially the question of whether there is a "core human microbiome", meaning, some aspect of microbial genome diversity that all healthy people share. Yet, nearly all studies to date are limited to clinical samples from developed nations. These samples have an ascertainment bias because the natural state of human microbiome diversity is disrupted by medications such as antibiotics, and likely, introductions of microbes from global food resources. The proposed study is unique in that the samples to be studied lack this bias. Samples are to be analyzed using state of the art genome technologies, including a novel gene array. The specific aims are (AIM1) to collect fecal samples and record dietary information from three remote contemporary native Peruvian communities, each living in three very different environments: coastal desert, high-elevation Andean grasslands, and the Amazon jungle. In addition, one ancient community, where samples were retrieved from mummified remains, will be included. AIM2 is to characterize the bacterial species and bacterial genes observed in these samples using next generation genome sequencing and novel gene array technologies. AIM3 is to isolate key members of the microbial ecology. Hypotheses tested include: i) gut microbiomes are geographically structured, meaning the frequencies of microbial taxa and gene functions are more similar within a population than between populations. ii) microbiomes from remote communities have high species diversity. iii) newly recorded species of microflora are present within these remote ecologies. iv) microbes, atypical of gut ecologies in developed nations, assume functional roles within remote communities. Each of these hypotheses is a logical contribution to assessing human microbial diversity and the extent to which a core human microbiome exists.

描述（由申请人提供）：人类肠道是一个复杂的生态系统，拥有多达 1000 种不同的微生物物种。现在已知肠道内的微生物多样性影响人类健康和新陈代谢，希望对人类微生物多样性的研究将带来新的医学治疗方法。这项研究的目的是表征从生活在偏远地区的人们身上采集的肠道微生物样本。这一目标可能会对人类微生物组研究产生变革性影响，特别是是否存在“核心人类微生物组”的问题，即所有健康人共享的微生物基因组多样性的某些方面。然而，迄今为止几乎所有研究都仅限于来自发达国家的临床样本。这些样本存在确定偏差，因为人类微生物组多样性的自然状态受到抗生素等药物的破坏，也可能受到来自全球食物资源的微生物的引入的破坏。拟议的研究的独特之处在于要研究的样本没有这种偏见。将使用最先进的基因组技术（包括新型基因阵列）对样本进行分析。具体目标是（AIM1）从三个偏远的当代秘鲁土著社区收集粪便样本并记录饮食信息，每个社区生活在三种截然不同的环境中：沿海沙漠、高海拔安第斯草原和亚马逊丛林。此外，还将包括一个从木乃伊遗骸中取出样本的古代社区。 AIM2 旨在利用下一代基因组测序和新型基因阵列技术来表征这些样本中观察到的细菌种类和细菌基因。 AIM3 是分离微生物生态学的关键成员。测试的假设包括：i) 肠道微生物组具有地理结构，这意味着群体内微生物分类群和基因功能的频率比群体之间更相似。 ii) 来自偏远社区的微生物组具有高度的物种多样性。 iii) 新记录的微生物区系物种存在于这些偏远的生态系统中。 iv) 微生物是发达国家肠道生态的非典型微生物，在偏远社区中发挥着功能性作用。这些假设中的每一个都是对评估人类微生物多样性和核心人类微生物组存在程度的逻辑贡献。