Multicolor Fluorescent Analysis in Zebrafish

斑马鱼的多色荧光分析

• 批准号: 8142948
• 负责人: ALEXANDER F SCHIER
• 金额: $ 23.43万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8142948
• 关键词: Anatomy; Animal Model; Biological Models; Biological Process; Biology; Cell Nucleus; Cell division; Cells; Chimeric Proteins; Chromatin; Color; Congenital Abnormality; Development; Developmental Process; Disease; Effectiveness; Embryo; Genetic Recombination; Goals; Human; Image; Immunohistochemistry; Label; Larva; Lead; Life; Membrane; Methods; Mus; Nervous system structure; Neurons; Organ; Pattern; Physiological Processes; Proteins; Protocols documentation; Reagent; Reporter; Reporter Genes; Research; Resolution; Subcellular structure; Synapses; Technology; Testing; Time; Toxic effect; Work; Zebrafish; combinatorial; in vivo; innovation; insight; next generation; novel; public health relevance; tool; zebrafish development

DESCRIPTION (provided by applicant): The translucency of zebrafish embryos and larvae makes them ideally suited for the in vivo analysis of developmental and physiological processes. This study aims to develop tools for the multicolor fluorescent labeling of zebrafish cells. Previous studies have demonstrated that stochastic DNA recombination can lead to the expression of different combinations of fluorescent proteins in different cells. The combinatorial expression of fluorescent proteins endows cells with many colors, thereby providing a way to distinguish adjacent cells and visualize cellular interactions. We will develop tools that make this technology widely and easily applicable to the study of zebrafish development and anatomy (Aim 1) and generate next-generation reagents and protocols that allow the sophisticated application of this technology to various aspects of zebrafish biology (Aim 2). PUBLIC HEALTH RELEVANCE: Zebrafish is a vertebrate model organism that shares many biological processes with humans. Research in zebrafish has provided important insights into the causes of birth defects and disease. The proposed work is to enhance the utility of this model system by developing tools to label cells in multiple different colors and follow their development.

描述（由申请人提供）：斑马鱼胚胎和幼虫的半透明性使其非常适合发育和生理过程的体内分析。本研究旨在开发用于斑马鱼细胞多色荧光标记的工具。先前的研究表明，随机DNA重组可以导致不同细胞中荧光蛋白的不同组合的表达。荧光蛋白的组合表达赋予细胞多种颜色，从而提供了一种区分相邻细胞和可视化细胞相互作用的方法。我们将开发工具，使该技术广泛、轻松地应用于斑马鱼发育和解剖学的研究（目标 1），并生成下一代试剂和方案，使该技术能够复杂地应用于斑马鱼生物学的各个方面（目标 2） 。 公共健康相关性：斑马鱼是一种脊椎动物模式生物，与人类有许多相同的生物过程。对斑马鱼的研究为了解出生缺陷和疾病的原因提供了重要的见解。建议的工作是通过开发工具以多种不同颜色标记细胞并跟踪它们的发展来增强该模型系统的实用性。