Regulated Assembly of Subcellular Structures in alpha-Proteobacteria

α-变形菌亚细胞结构的调控组装

• 批准号: 8101439
• 负责人: Joseph Chiung-Chu Chen
• 金额: $ 3.47万
• 依托单位: SAN FRANCISCO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8101439
• 关键词: Affect; Alphaproteobacteria; Automobile Driving; Bacteria; Bacterial Proteins; Biogenesis; Brucella; Caulobacter crescentus; Cell division; Cells; Cellular Morphology; Development; Ensure; Environment; Fabaceae; Flagella; Funding; Future; Genes; Goals; Homologous Protein; Infection; Institution; Laboratories; Location; Melilotus; Mentors; Mentorship; Microbe; Modeling; Molecular Structure; Mutation; Nitrogen; Nodule; Organelles; Organism; Pathway interactions; Pattern; Phenotype; Pilum; Plant Roots; Plants; Polar Regions; Positioning Attribute; Principal Investigator; Proteins; Proteobacteria; Public Health; Publications; Regulation; Regulatory Pathway; Research; Rickettsia; Role; Scientist; Senior Scientist; Sinorhizobium meliloti; Students; Subcellular structure; Surface; Symbiosis; System; Time; Training; Virulence Factors; Work; antimicrobial; genetic regulatory protein; improved; mutant; novel; pathogen; post-doctoral training; prevent; protein complex; skills; success; transmission process

DESCRIPTION (provided by applicant): Bacterial cells have evolved multiple regulatory mechanisms to ensure that their subcellular structures and organelles, such as pili and flagella, are assembled and positioned correctly. Our goal is to determine how the core components of a conserved regulatory system, and their interactions, have been adapted to serve the particular needs of different organisms. In Caulobacter crescentus, the protein components of this regulatory system (PodJ, DivJ, and PleC) follow defined patterns of subcellular localization that contribute to asymmetric cell morphology: organelles specifically develop at one pole but not the other. The regulatory components are conserved in Sinorhizobium meliloti, a related bacterium that induces nodule formation in plant roots during symbiosis. However, S. meliloti cells are morphologically symmetric compared to C. crescentus. We will examine how these conserved components function in S. meliloti to regulate the assembly of surface organelles and how this regulation affects host-microbe interaction. (1) We will first determine the localization patterns of the regulatory proteins and the phenotypes of null mutants that lack the proteins. (2) We will then detect and analyze changes in the mutants' transcriptional profiles to see if the proteins control expression of similar genes in different species. (3) We will also assess whether the mutations disrupt interaction between the bacterium and its hosts, preventing effective nodulation. In addition to these research objectives, the principal investigator aims to achieve the following developmental objectives under the guidance of a senior scientist: (4) establish an independent research group, (5) enhance mentoring skills, and (6) improve the quality of research. Relevance to Public Health: The proposed research is expected to reveal the driving principles of a regulatory pathway that exists in multiple bacterial species, including pathogens. Components of the pathway may serve as targets for new antimicrobial compounds.

描述（由申请人提供）：细菌细胞已经发展出多种调节机制，以确保其亚细胞结构和细胞器（例如pili和鞭毛）得到正确组装并正确定位。我们的目标是确定保守监管系统的核心组成部分及其相互作用是如何适应不同生物体的特定需求的。在Caulobacter Crescentus中，该调节系统的蛋白质成分（PODJ，DIVJ和PLEC）遵循有助于非对称细胞形态的亚细胞定位模式：细胞器特异性在一个极点发展，而不是另一极。调节成分是在Meliloti的Sinorhizobium Meliloti中保守的，这是一种相关的细菌，可在共生期间诱导植物根中结节形成。然而，与新月梭状芽胞杆菌相比，Meliloti细胞在形态上是对称的。我们将研究这些保守的成分如何在Meliloti中起作用以调节表面细胞器的组装，以及该调节如何影响宿主 - 微生物相互作用。 （1）我们将首先确定调节蛋白的定位模式和缺乏蛋白质的无效突变体的表型。 （2）然后我们将检测和分析突变体转录谱的变化，以查看蛋白质是否控制着不同物种中相似基因的表达。 （3）我们还将评估突变是否破坏细菌与其宿主之间的相互作用，以防止有效结节。除了这些研究目标外，首席研究人员还旨在在高级科学家的指导下实现以下发展目标：（4）建立一个独立的研究小组，（5）提高指导能力，（6）提高研究质量。 与公共卫生的相关性：拟议的研究有望揭示在包括病原体在内的多种细菌物种中存在的调节途径的驾驶原理。该途径的成分可以作为新的抗菌化合物的靶标。

项目成果

Trapping and rotating microparticles and bacteria with moiré-based optical propelling beams.

• DOI: 10.1364/boe.3.001891
• 发表时间: 2012-08-01
• 期刊: Biomedical optics express
• 影响因子: 3.4
• 作者: Zhang P;Hernandez D;Cannan D;Hu Y;Fardad S;Huang S;Chen JC;Christodoulides DN;Chen Z
• 通讯作者: Chen Z