IKBKAP function in SNS development
SNS开发中的IKBKAP功能
基本信息
- 批准号:8128471
- 负责人:
- 金额:$ 18.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-01 至 2013-08-31
- 项目状态:已结题
- 来源:
- 关键词:AdultAffectAfferent NeuronsAgeAnimal ModelAutonomic Nervous System PartAutonomic nervous systemAxonBehavioralCancerousCellsCessation of lifeChildhoodClinicalComplexDefectDevelopmentDifferentiation and GrowthDiseaseDysautonomiasEmbryoEscherichia coliExonsFamilial DysautonomiaFutureGene Expression RegulationGenesGeneticHereditary DiseaseHereditary Sensory NeuropathyHomeostasisHumanIn VitroIntronsKnock-outKnockout MiceLaboratoriesLaboratory ResearchLeadLentivirus VectorMaintenanceMalignant - descriptorMediatingMethodsMissionModelingMusMutagenesisMutationNerve DegenerationNervous system structureNeuritesNeurodegenerative DisordersNeuronsOnline Mendelian Inheritance In ManOrganPartner in relationshipPathogenesisPhenotypePhysiologicalPoint MutationProcessProteinsQuality of lifeRNA Polymerase IIRNA SplicingRecombinantsRegulator GenesReporterResourcesRoleSensorySignal TransductionSiteSmall Interfering RNASourceStagingSubfamily lentivirinaeSympathetic Nervous SystemTechniquesTestingTissuesTranscriptTranscriptional RegulationTransfectionTransgenic MiceTransgenic OrganismsUnconscious StateUniversitiesautonomic neuropathybasecell motilitycell typedesignembryonic stem cellexperiencefunctional losshomologous recombinationin vivoinsightloss of functionmRNA Stabilitymigrationmouse modelneoplasticnerve supplynervous system developmentneuron developmentneuropathologyprematureprotein functionpublic health relevancerecombinaseresearch studyresponsesmall hairpin RNAtooltumorvector
项目摘要
DESCRIPTION (provided by applicant): The sympathetic nervous system (SNS) is a division of the autonomic nervous system that has a major role in tissue and organ homeostasis. It is the target of a wide variety of congenital and neurodegenerative diseases, and the source of several types of malignant pediatric and adult tumors. Millions of humans are afflicted with diseases involving the SNS, yet we understand very little about the mechanisms regulating growth and differentiation of sympathetic neurons or the mechanisms mediating the establishment and maintenance of target organ innervation. Familial Dysautonomia (FD, Riley Day Syndrome, HSAN3) is a devastating genetic autosomal recessive disease involving the sympathetic and sensory nervous systems. In greater than 99.5% of cases, it is caused by a single highly conserved point mutation of the IKBKAP gene. IKBKAP encodes a protein (IKAP) with very poorly characterized function. Here, we propose to study the function of IKBKAP in sympathetic and sensory neurons most affected by FD using in vitro and in vivo methods. This exploratory/developmental proposal is outlined with 2 specific aims: (1) to study IKAP function in sympathetic neurons in vivo by generating a mouse model to conditionally ablate (knockout) IKBKAP and (2) to examine the role of IKAP in growth, differentiation and axon outgrowth of sympathetic and sensory neurons in vitro. We anticipate that these studies will generate new insights into how IKAP functions during development of sympathetic and sensory neurons. Moreover, the conditional IKBKAP knockout mouse will provide a valuable tool for further studies aimed at elucidating prevailing gene regulatory networks controlled by IKAP that are involved in sympathetic and sensory nervous system development and maintenance in humans.
PUBLIC HEALTH RELEVANCE: The sympathetic nervous system (SNS) is critical for controlling many unconscious processes in the body and it is the target of a wide variety of developmental, degenerative and cancerous diseases. Familial Dysautonomia (FD) is a devastating genetic disease involving the sympathetic and sensory nervous systems and is caused by mutation of the IKBKAP gene. Very little is known about IKBKAP and its role in developing and adult sympathetic neurons. Here, we will generate an animal model that will make it possible to study in detail how mutation of IKBKAP leads to FD.
描述(由申请人提供):交感神经系统(SNS)是在组织和器官稳态中具有重要作用的自主神经系统的划分。它是多种先天性和神经退行性疾病的靶标,也是几种恶性小儿和成人肿瘤的来源。数以百万计的人患有涉及SNS的疾病,但我们对调节同情神经元的生长和分化的机制或介导目标器官支配的建立和维持的机制几乎没有理解。 家族性动物障碍(FD,Riley Day综合征,HSAN3)是涉及交感神经和感觉神经系统的毁灭性遗传常染色体隐性疾病。在超过99.5%的病例中,它是由IKBKAP基因的单个高度保守点突变引起的。 IKBKAP编码功能表征较差的蛋白质(IKAP)。在这里,我们建议研究IKBKAP在使用体外和体内方法受FD影响最大的交感神经和感觉神经元中的功能。该探索性/发育提案用2个具体目的概述了:(1)通过生成小鼠模型以有条件消融(基因敲除)IKBKAP和(2)检查IKAP在体内的作用,以检查IKAP在体内的作用,以检查IKAP在体内的作用,以检查IKAP在体内的作用,以检查IKAP在体内的生长,分化和轴突的作用。我们预计这些研究将产生有关IKAP在交感神经和感觉神经元发展过程中如何发挥作用的新见解。此外,有条件的IKBKAP敲除小鼠将为旨在阐明由IKAP控制的主要基因调节网络提供的进一步研究的有价值的工具,这些网络涉及人类的交感神经和感觉神经系统的发展和维持人。
公共卫生相关性:交感神经系统(SNS)对于控制体内许多无意识的过程至关重要,它是多种发育,退化性和癌性疾病的目标。家族性动物障碍(FD)是一种涉及交感神经和感觉神经系统的毁灭性遗传疾病,是由IKBKAP基因突变引起的。关于IKBKAP及其在发展和成人交感神经元中的作用知之甚少。在这里,我们将生成一个动物模型,该模型将有可能详细研究IKBKAP突变如何导致FD。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Retrograde nerve growth factor signaling abnormalities and the pathogenesis of familial dysautonomia.
- DOI:10.4103/1673-5374.306081
- 发表时间:2021-09
- 期刊:
- 影响因子:6.1
- 作者:Ghosh S;Li L;Tourtellotte WG
- 通讯作者:Tourtellotte WG
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WARREN G TOURTELLOTTE其他文献
WARREN G TOURTELLOTTE的其他文献
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$ 18.3万 - 项目类别:
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立即早期基因调控的发育/可塑性
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$ 18.3万 - 项目类别:
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