X-Ray Fluorescence Computer Tomography with Emission Tomography Apertures

带发射断层扫描孔径的 X 射线荧光计算机断层扫描

• 批准号: 8142859
• 负责人: Patrick Jean La Riviere
• 金额: $ 18.03万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8142859
• 关键词: Accounting; Algorithms; Animal Model; Animals; Area; Biological; Biological Testing; Biology; Calcium; Calibration; Catalysis; Cells; Characteristics; Collaborations; Collection; Computers; Contrast Media; Coupled; Data; Detection; Development; Diagnosis; Diagnostic; Diagnostic radiologic examination; Discipline; Elements; Fluorescence; Foundations; Generations; Genomics; Goals; Hour; Human; Image; Imaging Techniques; Indium; Individual; Insulin; Investigation; Ions; Islet Cell; Laboratories; Lead; Lighting; Magnetic Resonance Imaging; Manganese; Maps; Metals; Methods; Modeling; Motivation; Pancreas; Photons; Play; Positioning Attribute; Procedures; Reaction; Resolution; Roentgen Rays; Role; Sampling; Scanning; Scheme; Signal Transduction; Slice; Source; Spatial Distribution; Specimen; Speed; Synchrotrons; System; Techniques; Technology; Testing; Therapeutic; Time; Tissue Sample; Trace Elements; Trace metal; Universities; Work; X-Ray Computed Tomography; Zinc; base; beamline; cancer therapy; data acquisition; detector; experience; flexibility; image reconstruction; improved; in vivo; interest; molecular imaging; nanoparticle; novel; optical imaging; public health relevance; reconstruction; scale up; sensor; statistics; tomography; tool; Accounting; Algorithms; Animal Model; Animals; Area; Biological; Biological Testing; Biology; Calcium; Calibration; Catalysis; Cells; Characteristics; Collaborations; Collection; Computers; Contrast Media; Coupled; Data; Detection; Development; Diagnosis; Diagnostic; Diagnostic radiologic examination; Discipline; Elements; Fluorescence; Foundations; Generations; Genomics; Goals; Hour; Human; Image; Imaging Techniques; Indium; Individual; Insulin; Investigation; Ions; Islet Cell; Laboratories; Lead; Lighting; Magnetic Resonance Imaging; Manganese; Maps; Metals; Methods; Modeling; Motivation; Pancreas; Photons; Play; Positioning Attribute; Procedures; Reaction; Resolution; Roentgen Rays; Role; Sampling; Scanning; Scheme; Signal Transduction; Slice; Source; Spatial Distribution; Specimen; Speed; Synchrotrons; System; Techniques; Technology; Testing; Therapeutic; Time; Tissue Sample; Trace Elements; Trace metal; Universities; Work; X-Ray Computed Tomography; Zinc; base; beamline; cancer therapy; data acquisition; detector; experience; flexibility; image reconstruction; improved; in vivo; interest; molecular imaging; nanoparticle; novel; optical imaging; public health relevance; reconstruction; scale up; sensor; statistics; tomography; tool

DESCRIPTION (provided by applicant): The overall goal of this proposal is to develop and implement faster and more accurate synchrotron-based X-ray fluorescence computed tomography (XFCT) methods for the mapping of trace metals in biological samples. Many endogenous metals play critical roles in signal transduction and reaction catalysis, while others are quite toxic even in trace quantities. The study of these metals in biology would benefit greatly from the 3D spatially resolved maps of trace element distribution provided by the methods being explored in the proposal. In addition, exogenous metals are often critical components of new in-vivo molecular imaging agents. The techniques proposed here would provide calibration and subcellular localization information critical for the continued advancement of these technologies. XFCT is a stimulated emission tomography (ET) method in which monochromatic synchrotron X-rays are used to stimulate emission of characteristic X-rays from a sample, and it has the ability to produce three-dimensional maps of the distribution of individual elements in a small, intact specimen. As practiced now, the principal limitation of XFCT is its long acquisition time (on the order of 1 or more hours per slice), which limits the ability to image multiple samples for sake of comparison and improved experimental statistics. The key motivation for this proposed effort is to develop a novel detection system for XFCT studies that has a greatly improved imaging speed (10 to 100 times faster) while maintain a reasonable imaging resolution and an excellent sensitivity to the trace elements of interest. The specific aims of the proposal are: 1. To develop an ET-based detection system for XFCT applications. 2. To develop novel XFCT image reconstruction strategies accounting for secondary and scatter-induced fluorescence and that allow for region of interest imaging 3. To test the system and algorithms developed on a problem of biological interest: determining the spatial distribution of manganese introduced in islet cells Upon completion, this project will provide a validated means to perform trace metal imaging in biological specimens at significantly higher throughput than currently achievable. The project will also provide a foundation for scaling the techniques up to in vivo trace metal imaging in animals and humans. PUBLIC HEALTH RELEVANCE: XFCT is a stimulated emission tomography (ET) method in which monochromatic synchrotron X-rays are used to stimulate emission of characteristic X-rays from a sample, and it has the ability to produce three-dimensional maps of the distribution of individual elements in a small, intact specimen. As practiced now, the principal limitation of XFCT is its long acquisition time (on the order of 1 or more hours per slice), which limits the ability to image multiple samples for sake of comparison and improved experimental statistics. The overall goal of this proposal is to develop and implement faster and more accurate synchrotron-based X-ray fluorescence computed tomography (XFCT) methods for the mapping of trace metals in biological samples. Many endogenous metals play critical roles in signal transduction and reaction catalysis, while others are quite toxic even in trace quantities. The study of these metals in biology would benefit greatly from the 3D spatially resolved maps of trace element distribution provided by the methods being explored in the proposal. In addition, exogenous metals are often critical components of new in-vivo molecular imaging agents. The techniques proposed here would provide calibration and subcellular localization information critical for the continued advancement of these technologies.

描述（由申请人提供）：该提案的总体目标是开发和实现基于同步加速器的X射线荧光计算机断层扫描（XFCT）方法，用于生物样品中痕量金属的映射。许多内源性金属在信号转导和反应催化中起着关键作用，而其他内源性金属也具有很大的毒性。这些金属在生物学中的研究将从提案中探索的方法提供的3D空间解析图中受益匪浅。另外，外源金属通常是新的体内分子成像剂的关键成分。这里提出的技术将提供校准和亚细胞定位信息，这对于这些技术的持续发展至关重要。 XFCT是一种刺激的发射断层扫描方法（ET）方法，其中单色同步子X射线用于刺激样品中特征X射线的发射，并且具有在小型，完整的小样本中产生单个元素分布的三维图。如今，XFCT的主要限制是其较长的获取时间（按照每切的1小时或更长时间），这限制了为比较和改进的实验统计数据对多个样品进行成像的能力。 这项提出的努力的关键动机是开发一个新的检测系统，用于XFCT研究，该系统具有大大提高的成像速度（快10到100倍），同时保持合理的成像分辨率和对感兴趣的痕迹元素的良好敏感性。该提案的具体目的是：1。为XFCT应用开发基于ET的检测系统。 2。为了开发新型的XFCT图像重建策略，考虑了二次和散射引起的荧光，并允许感兴趣的区域成像3。测试基于生物学兴趣问题的系统和算法开发的：确定在胰岛细胞中引入的锰的空间分布，该项目在完成时可以通过在生物学成像中表现出可观的金属成像。该项目还将为在动物和人类中的体内痕量金属成像扩展到缩放技术的基础。 公共卫生相关性：XFCT是一种刺激的发射断层扫描方法（ET）方法，其中单色同步加速器X射线用于刺激样品中特征X射线的发射，并且它具有产生小型，完整标本中单个元素分布的三维图。如今，XFCT的主要限制是其较长的获取时间（按照每切的1小时或更长时间），这限制了为比较和改进的实验统计数据对多个样品进行成像的能力。该提案的总体目标是开发和实现基于同步加速器的X射线荧光计算机断层扫描（XFCT）方法，用于生物样品中痕量金属的映射。许多内源性金属在信号转导和反应催化中起着关键作用，而其他内源性金属也具有很大的毒性。这些金属在生物学中的研究将从提案中探索的方法提供的3D空间解析图中受益匪浅。另外，外源金属通常是新的体内分子成像剂的关键成分。这里提出的技术将提供校准和亚细胞定位信息，这对于这些技术的持续发展至关重要。