CHEMICAL AND ENVIRONMENTAL EFFECTS ON MAMMALIAN SKIN

化学和环境对哺乳动物皮肤的影响

• 批准号: 7357368
• 负责人: Michael Peter Shakarjian
• 金额: $ 1.23万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357368
• 关键词: CHEMICAL; ENVIRONMENTAL; EFFECTS; MAMMALIAN; SKIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our current studies at the BRC involve the physiological function of the epidermal layer of mammalian skin and its response to various chemical and environmental stimuli. The mammalian epidermis is a multilayered organ, serving to control water loss and protect the organism from external toxins and pathogens. The maintenance of the skin¿s integrity is essential for health and survival of the organism, and loss of epidermal barrier function has been implicated in responses to chemical insult as well as a number of disorders, including psoriasis and atopic dermatitis. In the skin, basal keratinocytes, self-renewing cells located at the base of the epidermis, grow, differentiate, and give rise to the cell layers above. In this process an extracellular pH gradient is formed, in which the outermost differentiated layers are most acidic. Acidification is driven by the epidermal cell proton extrusion, creating an environment essential for barrier integrity. We were interested in examining the mechanisms involved in extracellular acidification by keratinocytes, and recognized that NCRR BioCurrents Research Center had resources that were uniquely suited to monitoring extracellular pH from these cells.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一，该子项目和研究者 (PI) 可能已从其他 NIH 来源获得主要资助，因此可以在其他 CRISP 机构中得到体现。列出的是该中心，该中心不一定是研究者的机构。我们目前在 BRC 的研究涉及哺乳动物皮肤表皮层的生理功能及其对各种化学和环境刺激的反应。器官，用于控制水分流失并保护有机体免受外部毒素和病原体的侵害。表皮屏障功能的完整性对于生物体的健康和生存至关重要，表皮屏障功能的丧失与化学损伤的反应以及许多疾病有关，包括牛皮癣和特应性皮炎。位于表皮底部的细胞生长、分化并产生上面的细胞层，在此过程中形成细胞外 pH 梯度，其中最外层的分化层呈酸性。酸化是由表皮细胞质子挤出驱动的，创造了一个对于屏障完整性至关重要的环境。我们对检查角质形成细胞参与细胞外酸化的机制很感兴趣，并认识到 NCRR 生物电流研究中心拥有独特适合监测细胞外 pH 值的资源。这些细胞。