Supplement for Role of Environmental Weathering and Gastrointestinal Digestion on the Bioavailability and Toxicity of Microplastic and Cadmium Mixtures

补充环境风化和胃肠消化对微塑料和镉混合物的生物利用度和毒性的作用

• 批准号: 10854398
• 负责人: Jorge Gonzalez Estrella
• 金额: $ 22.04万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-15 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10854398
• 关键词: Address; Adverse effects; Affect; Affinity; Attention; Binding; Biological Availability; Caco-2 Cells; Cadmium; Cell Line; Cells; Cellular biology; Chemicals; Chemistry; Collaborations; Communities; Complement; Consumption; Data; Digestion; Dose; Ecosystem; Endocytosis; Endosomes; Environment; Environmental Engineering technology; Environmental Pollutants; Fishes; Fourier Transform; Fresh Water; Gas Chromatography; Gastrointestinal Process; Goals; Graduate Degree; Health; Health Hazards; Heavy Metals; Homeostasis; Human; In Vitro; Ingestion; Intestines; Knowledge; Learning; Lindane; Link; Liquid substance; Lysosomes; Mass Chromatography; Metabolism; Metals; Methodology; Mineral Waters; Modification; Oklahoma; Organic Chemicals; Organism; Physiological; Physiology; Plastics; Polyethylenes; Polypropylenes; Polyvinyl Chloride; Postdoctoral Fellow; Process; Reporting; Research; Research Personnel; Risk; Role; Route; Scanning Electron Microscopy; Scientist; Specific qualifier value; Spectrophotometry; Spectrum Analysis; Students; Supervision; Surface; System; Techniques; Testing; Toxic effect; Toxicology; Trace metal; Training; Weather; World Health Organization; acute toxicity; aged; aquatic organism; aqueous; bioaccumulation; career; cellular imaging; cytotoxicity; design; environmental chemical; environmental chemistry; environmental toxicology; gastrointestinal; graduate student; innovation; intestinal epithelium; light scattering; metal metabolism; meter; novel; parent grant; pollutant; skills; student training; theories; tool; ultraviolet; undergraduate student; uptake; zeta potential

Project Summary of Diversity Supplement In this supplement, the postdoctoral fellow candidate Dr. Justin Scott will carry out a project with the overall goal of understanding the sorption of organic compounds onto weathered MPs and nanoplastics (NPs), and MPs and NPs bioaccumulation and toxicity in fish (RTgutGC) and human (Caco2) intestinal cell lines. This will allow us to better understand their transformation and fate after ingestion and determine exposure risk and potential adverse effects. Justin will complement his toxicological training by learning advanced spectroscopy and environmental chemistry methodologies under the supervision of Dr. Jorge Gonzalez Estrella. Through the incorporation of analytical techniques such as dynamic light scattering (DLS), zeta potential, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and pyrolysis gas chromatography mass spectrophotometry (py-GC-MS) we will gain a better understanding of MP and NPs reactivity and adverse effects. Importantly, the reactivity of specific weathered (i.e., UV aged and oxidized) MPs/NPs types (i.e., polyethylene, polyvinyl chloride, and polypropylene) with specific organic chemical mixtures (i.e., lindane, dichlorodiphenyldichloroethylene (DDE), and azoxystrobin) will be determined. In addition, the adverse effect of MPs/NPs alone or in co-exposure with organics will be evaluated using a physiologically relevant exposure system which includes in vitro digestion and intestinal cells lines. We hypothesize that environmental weathering of MPs and NPs will increase sorption of organic chemical mixtures onto them, which will increase the contaminant uptake by the intestinal epithelium. Moreover, gastrointestinal and digestive processes will allow desorption of chemicals from MPs and NPs and an increase in bioaccumulation and cytotoxicity. Aim .1 Evaluate the reactivity of weathered MP and NP with organic chemical mixtures in exposure media; Aim 2: Evaluate the Fate of organic chemical mixtures and MPs/NPs in the exposure system; and Aim 3: Evaluate the role of gastrointestinal digestion processes on MPs/NPs reactivity with organic chemicals and effect in intestinal cells.

多样性补充项目概要 在本增刊中，博士后研究员贾斯汀·斯科特博士将开展一个项目，该项目的总体目标是 了解有机化合物在风化 MP 和纳米塑料 (NP) 上的吸附作用的目标，以及 MP 和 NP 在鱼类 (RTgutGC) 和人类 (Caco2) 肠道细胞系中的生物累积和毒性。这将 让我们更好地了解它们摄入后的转变和命运，并确定暴露风险和 潜在的不利影响。贾斯汀将通过学习先进的光谱学来补充他的毒理学培训 和环境化学方法学，在 Jorge Gonzalez Estrella 博士的监督下进行。通过 结合动态光散射 (DLS)、zeta 电位、傅里叶变换等分析技术 红外 (FTIR)、扫描电子显微镜 (SEM) 和热解气相色谱质谱 分光光度法 (py-GC-MS) 我们将更好地了解 MP 和 NP 的反应性和不良反应 影响。重要的是，特定风化（即紫外线老化和氧化）MP/NP 类型（即 聚乙烯、聚氯乙烯和聚丙烯）与特定有机化学混合物（即林丹、 将测定二氯二苯基二氯乙烯（DDE）和嘧菌酯）。此外，还有不良影响 单独或与有机物共同暴露的 MP/NP 将使用生理相关暴露进行评估 系统，包括体外消化和肠细胞系。我们假设环境 MP 和 NP 的风化会增加有机化学混合物对其的吸附，从而增加 肠上皮吸收污染物。此外，胃肠道和消化过程也会 允许化学物质从 MP 和 NP 中解吸，并增加生物蓄积性和细胞毒性。目标.1 评估风化 MP 和 NP 与暴露介质中有机化学混合物的反应性；目标 2： 评估暴露系统中有机化学混合物和 MP/NP 的命运；目标 3：评估 胃肠道消化过程对 MPs/NPs 与有机化学物质的反应性的作用以及对 肠细胞。