Metagenomics of the Microbiome in Oral Health and Disease

口腔健康和疾病中微生物组的宏基因组学

基本信息

批准号：
8128573
负责人：
Karen E. Nelson
金额：
$ 60.37万
依托单位：
J. CRAIG VENTER INSTITUTE, INC.
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-08-16 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8128573
关键词：
Accounting Acids Address Affect Attention Behavioral Biochemical Biochemical Pathway Categories Child Childhood Chronic Disease Code Communities Competence DNA Sequence Data Data Set Dental Care Dental Plaque Dental caries Development Diagnostic Dietary Practices Disease Disease model Etiology Exposure to Future Gene Expression Profile Gene Targeting Genes Genetic Transcription Genomics Goals Human Individual Infant Investigation Knowledge Link Longitudinal Studies Metabolic Pathway Metabolism Metagenomics Methodology Microbe Microbial Biofilms Microbial Genetics Modeling Monitor Mouth Diseases Natural History Oral cavity Oral health Organism Participant Pathway interactions Pattern Positioning Attribute Production Regulatory Pathway Relative (related person)Research Research Project Grants Role Streptococcus mutans Techniques Time Tooth Diseases Transcript Twin Multiple Birth Water Work acid stress bacteriocin biological adaptation to stress comparative deciduous tooth gene function mRNA Differential Displays metagenomic sequencing microbial microbiome novel oral microbiome public health relevance sugar transcriptomics treatment strategy

项目摘要

DESCRIPTION (provided by applicant): The development of dental caries in children provides a disease model that is well suited to a metagenomic investigation since the timeframe of caries development can be monitored within a defined study period during which longitudinal studies can be conducted. Dental caries has a well established link to discrete, but only partially characterized changes in the dental plaque microbiome. Our study endeavors to define the relative abundance of many species comprising the dental plaque microbiome in 60- 70 twin pairs discordant for dental caries in association and longitudinal studies. The study power afforded by the use of discordant twin pairs for dental caries is substantially greater than similar studies using unrelated individuals. Next, we will compare the caries-free and caries-active microbiomes by deep metagenomic sequencing to identify differentially abundant genes and metabolic pathways. Lastly, we will determine the functional relevance of differentially abundant species, strains and genes by conducting targeted meta-transcriptomic studies. The statistical significance of all of the datasets will be determined which will allow for the widespread identification of species, strains, genes and transcripts associated with dental caries and dental health. These approaches are likely to enhance our understanding of dental caries onset and development and may enable in the future the development of novel treatment strategies. PUBLIC HEALTH RELEVANCE: Our project directly addresses the identification of microbes that are responsible for the onset and development of dental caries. The data generated will allow us to identify numerous diagnostic markers for dental health and disease. This work may also define novel therapies for dental caries.

描述（由申请人提供）：儿童龋齿的发展提供了一种非常适合元基因组研究的疾病模型，因为可以在可以进行纵向研究的定义研究期内监测龋齿发育的时间范围。牙齿龋齿与离散的良好联系，但仅部分表征了牙菌斑微生物组的变化。我们的研究努力定义了许多物种的相对丰度，其中包括60-70个双胞胎对牙齿的牙齿微生物组的相对丰度，而与龋齿和纵向研究不一致。使用不和谐的双子对龋齿提供的研究能力大大比使用无关个体的类似研究要大得多。接下来，我们将通过深宏基测序比较无龋和龋齿活性微生物组，以鉴定差异丰富的基因和代谢途径。最后，我们将通过进行靶向的元转录组研究来确定差异丰富物种，菌株和基因的功能相关性。将确定所有数据集的统计意义，这将允许对与牙齿龋齿和牙齿健康相关的物种，菌株，基因和转录本的广泛识别。这些方法可能会增强我们对龋齿发作和发育的理解，并可能在未来发展新型治疗策略。公共卫生相关性：我们的项目直接解决了负责龋齿发作和发展的微生物的识别。产生的数据将使我们能够识别出许多用于牙齿健康和疾病的诊断标记。这项工作还可以定义龋齿的新型疗法。