IVIS Spectrum imager of bioluminescence and fluorescence

IVIS 生物发光和荧光光谱成像仪

基本信息

批准号：
7795614
负责人：
Kami Kim
金额：
$ 35.99万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-02-20 至 2011-02-19
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): An IVIS Spectrum imaging system will be purchased for use in a shared small animal imaging facility at the Albert Einstein College of Medicine. The IVIS imaging systems allow the real time imaging of small animals expressing luciferase or imaging of animals injected with cells or microbes expressing luciferase. The luciferase d-luciferin substrate is readily permeable into cells and tissue, resulting in bioluminescence imaging (BLI) of individualized organs and correlation to anatomic location. The Spectrum can image fluorescence as well (GFP, mCherry etc) in real time. The IVIS Spectrum will be used to monitor tumor or cellular migration and to monitor dissemination of a variety of pathogens. Because the luciferase or fluorescence activity can be monitored without sacrificing animals, longitudinal measurements can be made on individual mice over time. In addition 3 dimensional images can be acquired providing information about anatomic localization of reporters. The signal is proportional to cell number and can be readily quantified using the software provided with the IVIS machinery. The imaging technique will assist investigators interested in cancer biology, immunology and infectious diseases. Finally, the purchase of this instrument will enable investigators to minimize the number of animals required for their studies, as multiple images will be acquired for each animal in a study. Public Health Relevance: The Albert Einstein College of Medicine proposes to buy an IVIS Spectrum imaging instrument for a shared small animal imaging facility. This instrument will enable investigators to monitor cells expressing bioluminescent molecules or fluorescent molecules within small animals. Scientists will use this technique to understand how cancer cells metastasize, how infectious agents cause disease, how stem cells repopulate organs, and how mammals mount an immune response to pathogens or cancer.

描述（由申请人提供）：将在阿尔伯特·爱因斯坦医学院的共享小动物成像设施中购买IVIS频谱成像系统。 IVIS成像系统允许对表达荧光素酶的小动物进行实时成像或注入表达荧光素酶的细胞或微生物的动物的成像。荧光素酶D-荧光素底物很容易渗透到细胞和组织中，从而导致个性化器官的生物发光成像（BLI）以及与解剖位置的相关性。光谱也可以实时成像荧光（GFP，MCHERRY等）。 IVIS光谱将用于监测肿瘤或细胞迁移并监测各种病原体的传播。因为可以在不牺牲动物的情况下监测荧光素酶或荧光活性，因此可以随着时间的推移对单个小鼠进行纵向测量。另外，可以获取3个维图的图像，以提供有关记者解剖学位置的信息。该信号与单元号成正比，可以使用IVIS机械提供的软件容易量化。成像技术将帮助对癌症生物学，免疫学和传染病感兴趣的研究者。最后，购买该仪器将使研究人员能够最大程度地减少其研究所需的动物数量，因为在一项研究中将为每只动物获取多个图像。公共卫生相关性：阿尔伯特·爱因斯坦医学院（Albert Einstein of Medicine）建议购买用于共享小型动物成像设施的IVIS光谱成像工具。该仪器将使研究人员能够监测小动物中表达生物发光分子或荧光分子的细胞。科学家将使用这种技术来了解癌细胞如何转移，传染性药物如何引起疾病，干细胞如何重塑器官以及哺乳动物如何对病原体或癌症产生免疫反应。