Automated Nucleic Acid Extractor System:AutoGenFlex Star/AutoGen Quick-Gene 810

自动化核酸提取系统：AutoGenFlex Star/AutoGen Quick-Gene 810

• 批准号: 7793989
• 负责人: KENNETH CORNETTA
• 金额: $ 15.95万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-20 至 2011-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793989
• 关键词: Affect; Alzheimer' s Disease; Area; Arts; Bioinformatics; Cancer Center; Cells; Clinical; Clinical Medicine; Clinical Research; Collection; Contract Services; DNA; Development; Disease; Ensure; Equipment; Fees; Fostering; Funding; Genes; Genetic; Grant; Health; Housing; Indiana; Individual; Institutes; Institution; International; Investments; Maintenance; Medicine; National Center for Research Resources; Nucleic Acids; Process; RNA; Rare Diseases; Reagent; Research; Research Personnel; Sampling; Specimen; System; Time; Tissue Banking; Tissue Banks; Translational Research; United States National Institutes of Health; Universities; Work; analytical tool; base; biobank; cost; genetic analysis; improved; instrument; medical schools; member; novel; repository; sample collection; vector

DESCRIPTION (provided by applicant): This request is for funds to purchase a nucleic acid extraction system consisting of an AutoGenFlex Star automated DNA isolation system and the AutoGen Quick-Gene 810 RNA isolation unit. The instruments will be housed, managed, and maintained by the Indiana Clinical and Translational Sciences Institute (CTSI) within the IndianaCTSI Specimen Storage Facility. Biobanking has become an integral part of translational research. The rapid developments in high throughput sequencing along with novel bioinformatic analytical tools has led to an increase in the number of investigators performing novel genetic analysis. With this increase in analytical capacity, the limiting step has become the collection and isolation of nucleic acids. The equipment will facilitate health research for the CTSI member institutions: Indiana University (IU), Purdue University and the University of Notre Dame. All three members of the Indiana CTSI will share a common state-of-the-art repository that was built, in part, through a NCRR construction grant (C06-RR020128-01, 09/30/04 - 09/29/08, R.S. Fife, PI, K. Cornetta, Co-I). The facility will be housed at the IU School of Medicine. In addition to supporting individual investigators, the IU Simon Cancer Center (2P30CA082709), the National Cell Repository for Alzheimer Disease (5U24AG021886; PI: T. Foroud), the National Gene Vector Biorepository (P40 RR024928; PI K. Cornetta), and the Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center work closely with the Indiana CTSI repository efforts. As a result, the instrument system will have an impact on a much larger group of NIH funded investigators than those located in Indiana. The facility will serve as a recharge center for users and the service contract and reagent costs will be included in user fees. The CTSI will provide support for a technician dedicated to repository activities including DNA processing and equipment maintenance. By developing a common facility, the Indiana CTSI will facilitate sample collection and processing, promote sharing of samples, and assure compliance with international biobanking standards. The equipment requested in this proposal will improve consistency of biospecimens, increase capacity, and free up technician time for analytical work. Furthermore, the diverse group of investigators who will utilize this equipment will ensure the investment will facilitate basic and clinical research in almost every area of medicine. PROJECT NARRATIVE: Understanding the genetic basis of disease continues to foster the development of new treatments for both common and rare diseases. Automated extraction of DNA can facilitates genetic analysis, a benefit that will positively affect almost every area of clinical medicine and the capacity to provide RNA will allow Indiana investigators to pursue this rapidly expanding field. The equipment requested will improve the research for R01 funded investigators, national biobanking efforts, and Indiana CTSI investigators engaged in basic and clinical research.

描述（由申请人提供）：此请求用于购买核酸提取系统，该系统由 AutoGenFlex Star 自动 DNA 分离系统和 AutoGen Quick-Gene 810 RNA 分离装置组成。这些仪器将由印第安纳州临床和转化科学研究所 (CTSI) 在印第安纳州 CTSI 标本存储设施内安置、管理和维护。 生物样本库已成为转化研究的一个组成部分。高通量测序以及新型生物信息学分析工具的快速发展导致进行新型遗传分析的研究人员数量增加。随着分析能力的提高，限制步骤已成为核酸的收集和分离。该设备将促进 CTSI 成员机构的健康研究：印第安纳大学 (IU)、普渡大学和圣母大学。印第安纳州 CTSI 的所有三个成员将共享一个共同的最先进的存储库，该存储库部分是通过 NCRR 建设拨款建造的（C06-RR020128-01，2004 年 9 月 30 日 - 2008 年 9 月 29 日， R.S.法伊夫，PI，K.科内塔，Co-I）。该设施将设在印第安纳大学医学院。除了支持个人研究人员之外，印第安纳大学西蒙癌症中心 (2P30CA082709)、国家阿尔茨海默病细胞存储库 (5U24AG021886; PI: T. Foroud)、国家基因载体生物存储库 (P40 RR024928; PI K. Cornetta) 和印第安纳大学西蒙癌症中心治愈组织库的 Susan G. Komen 与印第安纳州密切合作CTSI 存储库工作。因此，该仪器系统将对 NIH 资助的研究人员产生比印第安纳州更大的研究人员群体的影响。该设施将作为用户的充值中心，服务合同和试剂费用将包含在用户费用中。 CTSI 将为专门从事 DNA 处理和设备维护等储存库活动的技术人员提供支持。通过开发通用设施，印第安纳州 CTSI 将促进样本收集和处理、促进样本共享并确保遵守国际生物样本库标准。该提案中要求的设备将提高生物样本的一致性，增加容量，并腾出技术人员的时间进行分析工作。此外，使用该设备的不同研究人员群体将确保这笔投资将促进几乎所有医学领域的基础和临床研究。 项目叙述：了解疾病的遗传基础将继续促进常见和罕见疾病新疗法的开发。 DNA 的自动提取可以促进遗传分析，这一好处将对临床医学的几乎每个领域产生积极影响，而提供 RNA 的能力将使印第安纳州的研究人员能够探索这个快速扩展的领域。所需的设备将改善 R01 资助的研究人员、国家生物样本库工作以及从事基础和临床研究的印第安纳州 CTSI 研究人员的研究。