In Vivo Imaging of Heart Disease and Host-Pathogen Processes

心脏病和宿主病原体过程的体内成像

• 批准号: 7796321
• 负责人: Roberta A. Gottlieb
• 金额: $ 39.55万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-18 至 2012-03-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796321
• 关键词: Anesthesia procedures; Animal Model; Animals; Arts; Award; Basic Science; Biological Process; Biomedical Research; Cell physiology; Cells; Clinical Trials Design; Collaborations; Detection; Development; Experimental Designs; Faculty; Fluorescence Microscopy; Funding; Future; Goals; Heart; Heart Diseases; Image; Immune response; Individual; Infarction; Infection; Inflammation; Lead; Life; Measures; Modality; Monitor; Mus; Patients; Process; Productivity; Proteins; Recruitment Activity; Reporter; Research; Research Personnel; Research Project Grants; Resolution; Signal Transduction; Stem cells; System; Time; Transgenic Animals; Transgenic Mice; Translations; United States National Institutes of Health; Virus Diseases; clinical application; cost; human disease; imaging modality; in vivo; instrument; member; mouse model; novel; pathogen; public health relevance

DESCRIPTION (provided by applicant): The Caliper Spectrum IVIS (in ivo imaging system) will be used to understand critical cellular processes in live mice using fluorescent or bioluminescent cells, proteins, or probes. Investigators are focused on heart disease or host response to infection, and have developed a range of transgenic animals or constructs that will enable them to utilize the imaging system for detection of cellular processes. This instrument images whole mice (under anesthesia) and can detect fluorescent or bioluminescent signals within the intact animal with a resolution of approximately 1 mm. This will allow investigators to detect nests of bioluminescent stem cells within the heart, to measure infarct size, or to monitor the progression of bacterial or viral infections over time. This instrument obviates the need to sacrifice animals at individual time points and will expand the modalities available for experimental design. Six NIH-funded investigators (representing 8 R01 awards and 3 investigators on a P01 award) will benefit from the instrument, and it is anticipated that additional faculty members will use the instrument in the future. The IVIS Spectrum will provide an important imaging modality that will extend the research capabilities of the investigators. Given the expertise of the group in fluorescence microscopy and in the development of transgenic mouse models for monitoring biological processes, it is expected that the IVIS will be rapidly incorporated into existing research projects. It will help to fulfill the important NIH goal of reducing the number of animals used in research, and this reduction in animal costs will extend the research dollars and increase productivity. The availability of the IVIS will facilitate new collaborations and will allow investigators to think about asking new questions, or taking novel experimental approaches. The availability of this state-of-the-art instrument will help SDSU recruit new biomedical research- oriented faculty as well as to retain its best investigators. This instrument will be extremely important to investigators wishing to apply their findings to patients, as the animal studies enhanced by the IVIS will lead the way to optimal clinical trial design. PUBLIC HEALTH RELEVANCE: The ability to conduct real-time, sequential in vivo imaging of small animals is an important extension of animal models developed to study human diseases, and a prerequisite to translation of basic science discoveries to clinical applications. This proposal is to request an in vivo imaging system (Caliper Spectrum) to study mice using fluorescent or bioluminescent reporters. The investigators contributing to this proposal will use the IVIS to study heart disease, inflammation, and infections.

描述（由申请人提供）：使用荧光或生物发光细胞，蛋白质或探针来理解活小鼠中的关键细胞过程的卡钳光谱IVI（IN IVO成像系统）。研究人员专注于心脏病或宿主对感染的反应，并开发了一系列转基因动物或结构，使它们能够利用成像系统检测细胞过程。该仪器对整只小鼠（在麻醉下）进行图像，并可以在完整动物中检测荧光或生物发光信号，分辨率约为1 mm。这将使研究人员能够检测心脏内生物发光干细胞的巢，以测量梗塞大小或监测细菌或病毒感染的进展。该仪器消除了在各个时间点牺牲动物的需要，并将扩大可用于实验设计的方式。 NIH资助的六名调查人员（代表8个R01奖项和3个调查人员获得P01奖）将从该工具中受益，预计将来会有更多的教职员工将使用该工具。 IVIS频谱将提供重要的成像方式，以扩展研究人员的研究能力。鉴于该组在荧光显微镜和转基因小鼠模型的开发中的专业知识鉴于监测生物学过程，预计IVI将迅速纳入现有的研究项目中。这将有助于实现减少研究中使用的动物数量的重要目标，而动物成本的降低将延长研究成本并提高生产率。 IVI的可用性将有助于新的合作，并将允许调查人员考虑提出新问题或采用新颖的实验方法。这种最先进的工具的可用性将有助于SDSU招募新的生物医学研究教师，并保留其最佳研究人员。对于希望将其发现的调查结果应用于患者的调查人员来说，该仪器将非常重要，因为IVIS增强的动物研究将导致最佳临床试验设计。 公共卫生相关性：进行实时，顺序对小动物进行连续成像的能力是开发用于研究人类疾病的动物模型的重要扩展，也是将基础科学发现转化为临床应用的先决条件。该建议是要求使用荧光或生物发光记者研究小鼠体内成像系统（卡钳光谱）。为该提案做出贡献的研究人员将使用IVI来研究心脏病，炎症和感染。