Flourescence and Luminescense Lifetime Instrument

荧光和发光寿命仪

• 批准号: 7794814
• 负责人: David L Farrens
• 金额: $ 21.68万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-18 至 2011-03-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794814
• 关键词: 13 year old; Anisotropy; Area; Arts; Biochemistry; Biological; Detection; Development; Dyes; Fluorescence; Funding; Goals; Lasers; Learning; Maintenance; Measurement; Measures; Metals; Methods; Nitrogen; Participant; Publications; Pump; Research; Research Personnel; Science; Scientist; Signal Transduction; System; Technology; Training; Visit; base; experience; graduate student; instrument; protein folding; protein structure function

DESCRIPTION (provided by applicant): We are requesting funds to purchase a PicoQuant FluoTime 200 fluorescence lifetime instrument. This instrument will augment and ultimately replace our 13 year old PTI LaserStrobe fluorescence lifetime system, which was originally purchased by the OHSU Department of Biochemistry as part of the recruitment package for Dr. Farrens. Presently, the PTI LaserStrobe is the only available system for measuring fluorescence lifetimes at OHSU and in the greater Portland area. It has proven instrumental in numerous publications and discoveries, and has served as a training opportunity for scientists and graduate students (both local and visiting) to learn about the use of fluorescence lifetimes in biological studies. However, the PTI instrument is now over 13 years old and is becoming unreliable, and maintenance is becoming difficult. Furthermore, the technology used (nitrogen-pumped dye laser with stroboscopic detection) is severely out of date, and presently does not enable precise measurements for lifetimes below ~ 500 ps, or anisotropy measurements. Thus, we need a new instrument to pursue the science being carried out by the 7 participants in this application. We considered several instruments, but ultimately we chose to go with a system from PicoQuant, as they have the longest experience in making laser-diode based fluorescence lifetime systems and their reputation for instrument stability and robustness is outstanding. The research that will be supported by this instrument includes subjects ranging from protein folding and assembly to signal transduction and metal transport, to the continued development of fluorescence methods for studying protein structure, function and dynamics. Our plan is to provide expertise, training and instrument access to scientists at OHSU and to researchers in the surrounding region. A modern state of the art fluorescence lifetime instrument is required to achieve this goal.

描述（由申请人提供）：我们要求资金购买Picquant Fluotime 200荧光寿命仪器。该乐器将增加并最终取代我们13岁的PTI Laserstrobe荧光寿命系统，该系统最初是由OHSU生物化学系购买的，作为Farrens博士招聘计划的一部分。目前，PTI LaserStrobe是唯一可在OHSU和大波特兰地区测量荧光寿命的系统。它已证明在众多出版物和发现中发挥了作用，并为科学家和研究生（本地和访问）提供了培训机会，以了解在生物学研究中使用荧光寿命。但是，PTI仪器现在已有13年的历史，并且变得不可靠，并且维护变得越来越困难。此外，使用的技术（具有频道检测的氮气染料激光器）非常过时，目前无法对低于500 PS的寿命或各向异性测量值进行精确的测量。因此，我们需要一种新工具来追求本申请中7名参与者正在进行的科学。我们考虑了几种仪器，但最终我们选择使用Picoquant的系统，因为它们在制作基于激光的荧光寿命系统方面拥有最长的经验及其在仪器稳定性和鲁棒性方面的声誉是出色的。该仪器将支持的研究包括从蛋白质折叠和组装到信号转导和金属转运的受试者，再到持续开发用于研究蛋白质结构，功能和动态的荧光方法。我们的计划是为OHSU的科学家以及周边地区的研究人员提供专业知识，培训和工具的访问。需要现代的荧光寿命仪器来实现这一目标。