Flourescence and Luminescense Lifetime Instrument

荧光和发光寿命仪

• 批准号: 7794814
• 负责人: David L Farrens
• 金额: $ 21.68万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-18 至 2011-03-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794814
• 关键词: 13 year old; Anisotropy; Area; Arts; Biochemistry; Biological; Detection; Development; Dyes; Fluorescence; Funding; Goals; Lasers; Learning; Maintenance; Measurement; Measures; Metals; Methods; Nitrogen; Participant; Publications; Pump; Research; Research Personnel; Science; Scientist; Signal Transduction; System; Technology; Training; Visit; base; experience; graduate student; instrument; protein folding; protein structure function

DESCRIPTION (provided by applicant): We are requesting funds to purchase a PicoQuant FluoTime 200 fluorescence lifetime instrument. This instrument will augment and ultimately replace our 13 year old PTI LaserStrobe fluorescence lifetime system, which was originally purchased by the OHSU Department of Biochemistry as part of the recruitment package for Dr. Farrens. Presently, the PTI LaserStrobe is the only available system for measuring fluorescence lifetimes at OHSU and in the greater Portland area. It has proven instrumental in numerous publications and discoveries, and has served as a training opportunity for scientists and graduate students (both local and visiting) to learn about the use of fluorescence lifetimes in biological studies. However, the PTI instrument is now over 13 years old and is becoming unreliable, and maintenance is becoming difficult. Furthermore, the technology used (nitrogen-pumped dye laser with stroboscopic detection) is severely out of date, and presently does not enable precise measurements for lifetimes below ~ 500 ps, or anisotropy measurements. Thus, we need a new instrument to pursue the science being carried out by the 7 participants in this application. We considered several instruments, but ultimately we chose to go with a system from PicoQuant, as they have the longest experience in making laser-diode based fluorescence lifetime systems and their reputation for instrument stability and robustness is outstanding. The research that will be supported by this instrument includes subjects ranging from protein folding and assembly to signal transduction and metal transport, to the continued development of fluorescence methods for studying protein structure, function and dynamics. Our plan is to provide expertise, training and instrument access to scientists at OHSU and to researchers in the surrounding region. A modern state of the art fluorescence lifetime instrument is required to achieve this goal.

描述（由申请人提供）：我们正在申请资金购买 PicoQuant FluoTime 200 荧光寿命仪器。该仪器将增强并最终取代我们已有 13 年历史的 PTI LaserStrobe 荧光寿命系统，该系统最初由 OHSU 生物化学系购买，作为 Farrens 博士招聘包的一部分。目前，PTI LaserStrobe 是 OHSU 和大波特兰地区唯一可用的测量荧光寿命的系统。事实证明，它在众多出版物和发现中发挥了重要作用，并为科学家和研究生（本地和访问）提供了培训机会，以了解荧光寿命在生物学研究中的应用。然而，PTI 仪器现已使用超过 13 年，变得越来越不可靠，维护也变得越来越困难。此外，所使用的技术（带有频闪检测的氮泵染料激光器）已经严重过时，目前无法实现低于约 500 ps 的寿命的精确测量或各向异性测量。因此，我们需要一种新的工具来研究该应用程序中 7 名参与者正在开展的科学工作。我们考虑了多种仪器，但最终选择了 PicoQuant 的系统，因为他们在制造基于激光二极管的荧光寿命系统方面拥有最长的经验，并且在仪器稳定性和稳健性方面享有盛誉。该仪器将支持的研究包括从蛋白质折叠和组装到信号转导和金属运输，以及用于研究蛋白质结构、功能和动力学的荧光方法的持续发展。我们的计划是为 OHSU 的科学家和周边地区的研究人员提供专业知识、培训和仪器使用权。实现这一目标需要现代最先进的荧光寿命仪器。