A Shared MicroCT Instrument for Virtual Histology Imaging of Bone and Soft Tissue

用于骨和软组织虚拟组织学成像的共享 MicroCT 仪器

• 批准号: 7793771
• 负责人: ROBERTO J FAJARDO
• 金额: $ 35.91万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2012-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793771
• 关键词: 3-Dimensional; Architecture; Bone Density; Bone Mineral Contents; Cancer Model; Communities; Data; Detection; Development; Embryology; Equipment; Evaluation; Funding; Grant; Histology; Image; Institutes; Institution; Medicine; Methods; Microscopic; Morphologic artifacts; National Center for Research Resources; Neoplasm Metastasis; Noise; Provider; Research; Research Personnel; Resolution; Resort; Resource Sharing; Sampling; Scanning; Science; Services; South Texas; Specimen; Speed; System; Testing; United States National Institutes of Health; X-Ray Computed Tomography; angiogenesis; base; bone; bone imaging; comparative; design; innovation; instrument; instrumentation; pre-clinical; programs; soft tissue; three dimensional structure; ultra high resolution; virtual

DESCRIPTION (provided by applicant): The instrument requested in this application is an ultra-high resolution microscopic computed tomography (microCT) scanner to evaluate 3-dimensional bone and soft tissue architecture from ex vivo specimens. This instrument, a Skyscan 1172 microCT scanner, is designed for en bloc imaging of bone for histomorphometry and determination of bone mineral density and bone mineral content. In addition, taking advantage of a unique method of microCT-based soft tissue Virtual Histology developed at our institution, one can image 3-dimensional structures of soft tissue for a wide variety of biomedical applications that include embryology, detection of micro-metastases in preclinical cancer models, and evaluation of neocapillaries during angiogenesis. None of the microCT equipment available in the South Texas region is capable of achieving the same resolution as the SkyScan 1172. Users who have attempted to collect data on the other instruments have been frustrated by the inability to capture data with sufficient detail and have therefore resorted to incurring the inconvenience and expense to send samples to commercial service providers. This situation is suboptimal due to that lack of biomedical expertise at the companies and the inability of the users to iteratively optimize scan parameters. Comparative tests of comparable systems for best spatial resolution, least noise, least artifacts, and best speed proved that the SkyScan 1172 was the most appropriate instrument to satisfy the needs of the UTHSCSA user community. As a shared resource for the UTHCSA campus, the SkyScan 1172 will be available to UTHSCSA investigators and partnering institutions throughout South Texas as a shared resource for the Institute for the Integration of Medicine and Science (NCRR CTSI, UL1 RR025767) as well as to academic investigators in other states. Immediately, the shared instrument would support 12 NIH grants as well as 10 other federally-funded regional and national grants however it is expected that a number of other research programs will take advantage of this system once it becomes available regionally. The development of microCT-based soft tissue Virtual Histology as a widely available application is considered a key innovation of this shared instrumentation grant.

描述（由申请人提供）：本申请中要求的仪器是超高分辨率显微计算机断层扫描（microCT）扫描仪，用于评估离体样本的 3 维骨骼和软组织结构。该仪器是 Skyscan 1172 microCT 扫描仪，专为骨骼整体成像而设计，用于组织形态测量以及骨矿物质密度和骨矿物质含量的测定。此外，利用我们机构开发的基于 microCT 的独特软组织虚拟组织学方法，可以对软组织的 3 维结构进行成像，用于各种生物医学应用，包括胚胎学、临床前微转移检测癌症模型以及血管生成过程中新毛细血管的评估。德克萨斯州南部地区可用的 microCT 设备均无法达到与 SkyScan 1172 相同的分辨率。尝试在其他仪器上收集数据的用户因无法捕获足够详细的数据而感到沮丧，因此不得不求助于其他仪器将样品发送给商业服务提供商会带来不便和费用。由于公司缺乏生物医学专业知识以及用户无法迭代优化扫描参数，这种情况并不理想。对同类系统进行的最佳空间分辨率、最小噪声、最小伪影和最佳速度的比较测试证明，SkyScan 1172 是满足 UTHSCSA 用户社区需求的最合适仪器。作为 UTHCSA 校园的共享资源，SkyScan 1172 将提供给德克萨斯州南部的 UTHSCSA 研究人员和合作机构，作为医学与科学一体化研究所 (NCRR CTSI、UL1 RR025767) 以及学术界的共享资源其他州的调查员。该共享工具将立即支持 12 项 NIH 拨款以及 10 项其他联邦资助的地区和国家拨款，但预计一旦该系统在地区可用，许多其他研究项目也将利用该系统。基于 microCT 的软组织虚拟组织学的开发作为一种广泛使用的应用程序被认为是该共享仪器拨款的关键创新。