Request for a Spectral Confocal Microscope

索取光谱共焦显微镜

基本信息

批准号：
7793953
负责人：
THOMAS C RICH
金额：
$ 39.74万
依托单位：
UNIVERSITY OF SOUTH ALABAMA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-06 至 2011-05-05
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Eleven NIH-funded investigators and one junior investigator with a pending NIH application are seeking support under the NCRR Shared Instrumentation Grant Program to obtain a Nikon A1 laser scanning confocal microscope. The Nikon microscope is justified because the two other confocal microscopes available at the University of South Alabama - a nine-year-old Leica TCS SP2 spectral confocal microscope and a five year old PerkinElmer Ultraview RS-3 spinning disk confocal microscope - are heavily used by thirty labs and more than fifty individuals. As such, access to these microscopes has become a rate-limiting step in conducting research. This limitation has worsened because increased usage and age have led to component failure and subsequent down time of the Leica system. In addition, neither microscope is capable of obtaining high resolution confocal images of preparations in environmental chambers or making live-cell, time lapse measurements of multiple fluorophores. The steering committee of the BioImaging Core Facility has identified the Nikon A1 laser scanning confocal microscope as the system best suited for our needs, because of its high acquisition speed, high-spatial resolution, incorporation of an acousto-optical tunable filter to control laser intensity, and ability to simultaneously acquire spectra divided into 32 separate wavelength bins of 2.5, 5, or 10 nm, and Nikon's excellent customer service at the University of South Alabama. The Nikon microscope will support a group of investigators conducting research in the areas of Lung Endothelial Biology, Vascular Smooth Muscle Biology, and Cancer Biology. As such, the work is highly relevant to understanding the underlying factors contributing to hypertension, vascular disease, COPD, and breast cancer, as well as the development of treatments for these conditions. The system will be housed in the BioImaging Core Facility with the two current confocal microscopes. The USA College of Medicine has committed to refurbish space, and to provide financial support for a three-year extended service contract with Nikon, and a Ph.D. level manager to run the BioImaging Core Facility. This individual will assist researchers with the use of the Nikon system, as well as other aspects of experimental design. The BioImaging Core Facility will ensure appropriate use and sharing of the system. The addition of the new generation confocal microscope will greatly expand the imaging capabilities available to NIH-funded researchers present on University of South Alabama campus.

描述（由申请人提供）：11名NIH资助的调查人员和一名具有NIH申请的初级调查员正在根据NCRR共享仪器赠款计划寻求支持，以获得尼康A1激光扫描共聚焦显微镜。尼康显微镜是有道理的，因为南阿拉巴马大学提供的另外两个共聚焦显微镜 - 九岁的Leica TCS SP2 Sp2频谱共聚焦显微镜和五岁的Perkinelmer Ultraview RS-3 Spinning Disk Compocal Microscope-被刻薄的实验室大量使用。因此，对这些显微镜的访问已成为进行研究的限制步骤。这种限制恶化了，因为使用和年龄增加导致组件故障以及随后的左撇子系统停机时间。此外，没有显微镜能够在环境室中获得高分辨率共焦图像，或进行活细胞，对多个荧光团的时间流失测量。生物成像核心设施的指导委员会已经确定了尼康A1激光扫描共聚焦显微镜是最适合我们需求的系统，因为它具有高获取速度，高空间分辨率，将声学调谐过滤器纳入以控制激光强度，以控制激光的强度，并能够同时获得32 nnm bins n wevelleptra nem bins，bins of 2.尼康在南阿拉巴马大学出色的客户服务。尼康显微镜将支持一组研究人员在肺内皮生物学，血管平滑肌生物学和癌症生物学领域进行研究。因此，这项工作与理解有助于高血压，血管疾病，COPD和乳腺癌的潜在因素以及这些疾病治疗的发展高度相关。该系统将安置在两个当前共聚焦显微镜的生物成像核心设施中。美国医学院已致力于翻新空间，并为与尼康的三年扩展服务合同和博士学位提供财政支持。级别的经理运行生物成像核心设施。该人将帮助研究人员使用尼康系统以及实验设计的其他方面。生物成像核心设施将确保适当使用和共享系统。新一代共聚焦显微镜的添加将大大扩展南阿拉巴马大学校园内的NIH资助的研究人员可用的成像功能。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Dynamic Ca(2+) signal modalities in the vascular endothelium.

DOI：
10.1111/j.1549-8719.2012.00180.x
发表时间：
2012-07
期刊：
Microcirculation (New York, N.Y. : 1994)
影响因子：
0
作者：
Taylor MS;Francis M;Qian X;Solodushko V
通讯作者：
Solodushko V

ASIC subunit ratio and differential surface trafficking in the brain.

DOI：
10.1186/s13041-016-0185-7
发表时间：
2016-01-08
期刊：
Molecular brain
影响因子：
3.6
作者：
Wu J;Xu Y;Jiang YQ;Xu J;Hu Y;Zha XM
通讯作者：
Zha XM

Hyperspectral imaging microscopy for identification and quantitative analysis of fluorescently-labeled cells in highly autofluorescent tissue.

DOI：
10.1002/jbio.201100066
发表时间：
2012-01
期刊：
JOURNAL OF BIOPHOTONICS
影响因子：
2.8
作者：
Leavesley, Silas J.;Annamdevula, Naga;Boni, John;Stocker, Samantha;Grant, Kristin;Troyanovsky, Boris;Rich, Thomas C.;Alvarez, Diego F.
通讯作者：
Alvarez, Diego F.

The interaction between the first transmembrane domain and the thumb of ASIC1a is critical for its N-glycosylation and trafficking.