ARIA II FLOW CYTOMTR: 3 LASER VIOW/ACDU

ARIA II 流式细胞仪：3 激光 VIOW/ACDU

• 批准号: 7794519
• 负责人: WEIMIN ZHONG
• 金额: $ 42.84万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2012-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794519
• 关键词: Animal Model; Antibodies; Arts; Bacteria; Behavior; Biological; Biological Process; Cell Culture Techniques; Cell Cycle Regulation; Cell Separation; Cell division; Cells; Cellular biology; Chickens; Color; Complex; Cytoskeleton; Defect; Development; Developmental Biology; Developmental Cell Biology; Epithelial; Flow Cytometry; Funding; Gene Expression; Individual; Investigation; Label; Lasers; Maintenance; Microscopy; Molecular Motors; Mus; Neural Crest; Neurons; Population; Population Analysis; Proteins; RNA; Research; Research Activity; Research Personnel; Science; Side; Spinal; Staining method; Stains; Stem cells; Techniques; United States National Institutes of Health; Universities; Yeasts; Zebrafish; cell motility; experience; instrument; instrumentation; nerve stem cell; programs; public health relevance; research study; self-renewal; somitogenesis

DESCRIPTION (provided by applicant): We are requesting funds for the acquisition of a BD FACSAria II Cell-sorting instrument. The researchers on the Science Hill campus of Yale University have extensive microscopy experience for their cell, stem cell and developmental biology research programs. These users would like to extend their extensive microscopy experience studying cell and developmental biology questions to use flow cytometry analyses for quantitative analysis of cell populations and cell isolation for clonal studies. The availability of this state-of-the-art flow cytometry instrument would heighten the research of users of this proposal by allowing quantitative analysis of the expression of up to 10 fluorescently labeled molecules and/or antibody stained proteins in large populations of cells. Furthermore, this instrument will allow users to isolate up to 4 individual labeled populations of cells via cell sorting to use for various applications such as population analysis of clonal behavior in cell culture, of gene expression by RNA isolation, etc. The proposed instrument will be the only machine capable of modern multi-color analysis and cell sorting on the Science Hill campus of Yale University. The availability of this instrument on Science Hill will allow complex and cutting edge experiments to be performed using flow cytometry by making it an efficient and accessible technique to support the strong cell biology, stem cell, and developmental biology programs in multiple model organisms including chicken, mouse, zebrafish, yeast and bacteria. Research activities facilitated by this instrument will include the investigation of asymmetric cell division and self-renewal in neural stem cells (Weimin Zhong, PI), cytoskeleton and molecular motors (Mark Mooseker), biological networks (Thierry Emonet), origin and induction of neural crest stem cells (Martin Garcia-Castro), zebrafish somitogenesis (Scott Holley), maintenance and control of epithelial stem cells (Valerie Horsley), cytoskeletal dynamics and cellular motility (Tom Pollard), cell cycle regulation (Hochstrasser and Solomon) and axonal, dendritic and spinal abnormalities in neuronal developmental defects (Elke Stein). PUBLIC HEALTH RELEVANCE: The requested state-of-the-art instrument will have a significant impact on the research programs on the Science Hill side of the Yale campus by filling a significant void in our available instrumentation. Given the need to quantitatively analyze and isolate populations of cells in order to examine the complexities of biological processes, access to a state-of-the-art flow cytometry and cell sorting instrument will maximize the impact of currently funded NIH research.

描述（由申请人提供）：我们正在申请资金购买 BD FACSAria II 细胞分选仪。耶鲁大学科学山校区的研究人员在细胞、干细胞和发育生物学研究项目方面拥有丰富的显微镜经验。这些用户希望扩展他们在研究细胞和发育生物学问题方面丰富的显微镜经验，以使用流式细胞术分析进行细胞群的定量分析和克隆研究的细胞分离。这种最先进的流式细胞术仪器的可用性将通过允许对大量细胞中多达 10 种荧光标记分子和/或抗体染色蛋白质的表达进行定量分析，从而加强该提案用户的研究。此外，该仪器将允许用户通过细胞分选分离多达 4 个单独的标记细胞群体，以用于各种应用，例如细胞培养中克隆行为的群体分析、通过 RNA 分离进行基因表达的群体分析等。拟议的仪器将是耶鲁大学科学山校区唯一能够进行现代多色分析和细胞分选的机器。 Science Hill 上该仪器的可用性将允许使用流式细胞术进行复杂和前沿的实验，使其成为一种高效且易于使用的技术，以支持多种模型生物（包括鸡、小鼠、斑马鱼、酵母和细菌。该仪器促进的研究活动将包括研究神经干细胞的不对称细胞分裂和自我更新（钟伟民，PI）、细胞骨架和分子马达（Mark Mooseker）、生物网络（Thierry Emonet）、神经细胞的起源和诱导。嵴干细胞 (Martin Garcia-Castro)、斑马鱼体细胞发生 (Scott Holley)、上皮干细胞的维持和控制 (Valerie Horsley)、细胞骨架动力学和细胞运动（Tom Pollard）、细胞周期调节（Hochstrasser 和 Solomon）以及神经元发育缺陷中的轴突、树突和脊髓异常（Elke Stein）。 公共卫生相关性：所需的最先进仪器将填补我们现有仪器的重大空白，对耶鲁大学校园科学山一侧的研究项目产生重大影响。鉴于需要定量分析和分离细胞群以检查生物过程的复杂性，使用最先进的流式细胞术和细胞分选仪器将最大限度地发挥目前资助的 NIH 研究的影响。