ARIA II FLOW CYTOMTR: 3 LASER VIOW/ACDU

ARIA II 流式细胞仪：3 激光 VIOW/ACDU

• 批准号: 7794519
• 负责人: WEIMIN ZHONG
• 金额: $ 42.84万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2012-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794519
• 关键词: Animal Model; Antibodies; Arts; Bacteria; Behavior; Biological; Biological Process; Cell Culture Techniques; Cell Cycle Regulation; Cell Separation; Cell division; Cells; Cellular biology; Chickens; Color; Complex; Cytoskeleton; Defect; Development; Developmental Biology; Developmental Cell Biology; Epithelial; Flow Cytometry; Funding; Gene Expression; Individual; Investigation; Label; Lasers; Maintenance; Microscopy; Molecular Motors; Mus; Neural Crest; Neurons; Population; Population Analysis; Proteins; RNA; Research; Research Activity; Research Personnel; Science; Side; Spinal; Staining method; Stains; Stem cells; Techniques; United States National Institutes of Health; Universities; Yeasts; Zebrafish; cell motility; experience; instrument; instrumentation; nerve stem cell; programs; public health relevance; research study; self-renewal; somitogenesis

DESCRIPTION (provided by applicant): We are requesting funds for the acquisition of a BD FACSAria II Cell-sorting instrument. The researchers on the Science Hill campus of Yale University have extensive microscopy experience for their cell, stem cell and developmental biology research programs. These users would like to extend their extensive microscopy experience studying cell and developmental biology questions to use flow cytometry analyses for quantitative analysis of cell populations and cell isolation for clonal studies. The availability of this state-of-the-art flow cytometry instrument would heighten the research of users of this proposal by allowing quantitative analysis of the expression of up to 10 fluorescently labeled molecules and/or antibody stained proteins in large populations of cells. Furthermore, this instrument will allow users to isolate up to 4 individual labeled populations of cells via cell sorting to use for various applications such as population analysis of clonal behavior in cell culture, of gene expression by RNA isolation, etc. The proposed instrument will be the only machine capable of modern multi-color analysis and cell sorting on the Science Hill campus of Yale University. The availability of this instrument on Science Hill will allow complex and cutting edge experiments to be performed using flow cytometry by making it an efficient and accessible technique to support the strong cell biology, stem cell, and developmental biology programs in multiple model organisms including chicken, mouse, zebrafish, yeast and bacteria. Research activities facilitated by this instrument will include the investigation of asymmetric cell division and self-renewal in neural stem cells (Weimin Zhong, PI), cytoskeleton and molecular motors (Mark Mooseker), biological networks (Thierry Emonet), origin and induction of neural crest stem cells (Martin Garcia-Castro), zebrafish somitogenesis (Scott Holley), maintenance and control of epithelial stem cells (Valerie Horsley), cytoskeletal dynamics and cellular motility (Tom Pollard), cell cycle regulation (Hochstrasser and Solomon) and axonal, dendritic and spinal abnormalities in neuronal developmental defects (Elke Stein). PUBLIC HEALTH RELEVANCE: The requested state-of-the-art instrument will have a significant impact on the research programs on the Science Hill side of the Yale campus by filling a significant void in our available instrumentation. Given the need to quantitatively analyze and isolate populations of cells in order to examine the complexities of biological processes, access to a state-of-the-art flow cytometry and cell sorting instrument will maximize the impact of currently funded NIH research.

描述（由申请人提供）：我们要求资金以获取BD FACSARIA II单元格式仪器。耶鲁大学科学山校园的研究人员为其细胞，干细胞和发育生物学研究计划具有丰富的显微镜经验。这些用户希望扩展其广泛的显微镜经验研究细胞和发育生物学问题，以使用流式细胞仪分析进行定量分析细胞群体和细胞分离以进行克隆研究。这种最先进的流式细胞仪仪器的可用性将通过允许对大量细胞种群中多达10个荧光标记的分子和/或抗体染色蛋白的表达进行定量分析，从而提高该提案的使用者的研究。此外，该仪器将允许用户通过细胞分类隔离多达4个单独的细胞群体，用于用于各种应用，例如对细胞培养中克隆行为的种群分析，通过RNA隔离来隔离基因表达等。拟议的仪器将是唯一能够在Yale University的科学山丘校园内能够现代多色分析和细胞分类的机器。该乐器在科学山上的可用性将通过流式细胞仪进行复杂而尖端的实验，通过使其成为一种有效且易于使用的技术，以支持多种模型有机体，包括鸡，小鼠，斑马鱼，酵母，酵母和细菌的强大细胞生物学，干细胞和发育生物学程序。 Research activities facilitated by this instrument will include the investigation of asymmetric cell division and self-renewal in neural stem cells (Weimin Zhong, PI), cytoskeleton and molecular motors (Mark Mooseker), biological networks (Thierry Emonet), origin and induction of neural crest stem cells (Martin Garcia-Castro), zebrafish somitogenesis (Scott Holley), maintenance以及控制上皮干细胞（Valerie Horsley），细胞骨架动力学和细胞运动性（Tom Pollard），细胞周期调节（Hochstrasser和Solomon）以及轴突，轴突，树突状和脊柱异常，神经元缺陷（Elke Stein）。 公共卫生相关性：所需的最新工具将通过在我们可用的仪器中填补大量空隙来对耶鲁校园科学山的研究计划产生重大影响。鉴于需要定量分析和分离细胞的种群以检查生物过程的复杂性，因此获得最先进的流式细胞仪和细胞分选仪器的访问将最大程度地提高当前资助的NIH研究的影响。