Confocal imaging resources for the JHU Zebrafish Center

JHU 斑马鱼中心的共焦成像资源

• 批准号: 7794133
• 负责人: Steven D Leach
• 金额: $ 47万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-08 至 2011-07-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794133
• 关键词: American; Applications Grants; Biology; Cells; Development; Developmental Biology; Elements; Embryo; Faculty; Funding; Genetic; Genetic Medicine; Goals; Grant; Head; Health; Hour; Human; Image; Institutes; Investigation; Joints; Lead; Life; Maintenance; Malignant Neoplasms; Malignant neoplasm of pancreas; MicroRNAs; Microscope; Microscopy; National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; Natural regeneration; Neural Crest; Neuronal Differentiation; Noise; Pancreas; Protocols documentation; Recovery; Regulator Genes; Research Personnel; Resolution; Resources; Role; Scanning; Shipping; Ships; Signal Transduction; System; Time; Tissues; United States National Institutes of Health; Universities; Work; Zebrafish; base; cellular engineering; design; embryo tissue; instrumentation; medical schools; member

DESCRIPTION (provided by applicant): This S10 Shared Instrumentation Grant application seeks funding in support of confocal live imaging resources for the Johns Hopkin University's Center for Functional Investigation in Zebrafish, also known as FinZ Center. FinZ Center was established in 2008 under joint funding from the School of Medicine, the Institute for Cellular Engineering (ICE), and the Johns Hopkins McKusick-Nathans Institute of Genetic Medicine (IGM). The Center is currently led by three scientific co-directors, Steven Leach, Andrew McCallion and Michael Parsons, each of whom is a noted investigator in zebrafish genetics and zebrafish developmental biology. Together with Dr. Joshua Mendell, another IGM faculty member pursuing work in zebrafish, this group will comprise the Major User Group for the proposed Zeiss LSM 710 confocal microscope. These four Major Users are all highly regarded investigators in a variety of scientific fields with direct impact on human health, including pancreatic development, pancreatic cancer, neuronal differentiation, b-cell regeneration, computational prediction of gene regulatory sequences, neural crest biology and the role of microRNAs in human malignancy. Together, they serve as PI's or co-Pis on seven NIH R01 or P01 grants, funded by four different NIH Institutes (NIGM, NICHD, NIDDK and NCI). Based on the Major User Group's common need for live imaging of zebrafish embryos and other tissues in support of their NIH-funded work, this application seeks funding for a Zeiss LSM 710 3-channel spectral confocal system, with an environmental control system to support long-term imaging of zebrafish embryos, tissues and cells. Justification for this request is based on the following documented elements: 1) the Zeiss 710 system offers an unprecedented signal-to-noise ratio associated with a unique scan head design, providing the opportunity to apply high resolution spectral imaging to deep-seated zebrafish tissues; 2) the fact that Johns Hopkins Microscopy Core resources here at Johns Hopkins are already fully subscribed and therefore cannot accommodate the 10-36 hour time lapse imaging protocols required by the Major User Group; and 3) consistent with the broader goals of the American Recovery and Reinvestment Act, the ordering, shipping, installation, maintenance and daily use of this microscope will directly stimulate multiple segments of the U.S. economy, and lead to the direct hiring of new technical staff.

描述（由申请人提供）：此S10共享仪器赠款申请寻求资金，以支持约翰·霍普金大学在斑马鱼的功能调查中心共焦实时成像资源，也称为Finz Center。 Finz中心成立于2008年，由医学院，蜂窝工程学院（ICE）和Johns Hopkins McKusick-Nathans遗传医学研究所（IGM）成立。该中心目前由三个科学联合导演，史蒂文·里奇（Steven Leach），安德鲁·麦卡利恩（Andrew McCallion）和迈克尔·帕森斯（Michael Parsons）领导，每个人都是斑马鱼遗传学和斑马鱼发育生物学的著名研究者。该小组与另一位IGM教职员工Joshua Mendell博士一起，将构成拟议中的Zeiss LSM 710共共聚焦显微镜的主要用户组。这四个主要使用者都是对人类健康直接影响的各种科学领域的备受推崇的研究人员，包括胰腺发育，胰腺癌，神经元分化，B细胞再生，基因调节序列的计算预测，神经crest生物学的计算预测以及microRNAS在人类恶性中的作用。他们一起在七个NIH R01或P01赠款上充当PI或Co-Pis，由四个不同的NIH研究所（NIGM，NICM，NICHD，NIDDK和NCI）资助。基于主要用户组对斑马鱼胚胎和其他组织的实时成像的共同需求，以支持其NIH资助的工作，该应用程序为Zeiss LSM 710 3通道频谱共焦系统寻求资金，并具有环境控制系统，以支持Zebrafish胚胎，组织和细胞的长期成像。此请求的理由基于以下记录的元素：1）Zeiss 710系统提供了与独特的扫描头设计相关的前所未有的信噪比，从而提供了将高分辨率光谱成像应用于深座斑马鱼组织的机会； 2）Johns Hopkins的Johns Hopkins显微镜核心资源已经完全订阅，因此无法容纳主要用户组要求的10-36小时的时间衰变成像协议； 3）与《美国恢复和再投资法》的更广泛目标一致，订购，运输，安装，维护和日常使用该显微镜将直接刺激美国经济的多个细分市场，并导致直接雇用新的技术人员。