Acquisition of a Confocal Microscope for R.M Bock Laboratories

为 R.M Bock 实验室购买共焦显微镜

• 批准号: 7794338
• 负责人: Kevin William Eliceiri
• 金额: $ 39.22万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-22 至 2011-07-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794338
• 关键词: Cell physiology; Cellular biology; Equipment; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Funding; Housing; Institutes; Label; Laboratories; Lasers; Malignant Neoplasms; Microscope; Molecular Biology; Molecular Virology; Optics; Request for Proposals; Research; Research Personnel; Scanning; Site; System; Universities; Virus; Wisconsin; cellular engineering; instrument; instrumentation; member; novel; photoactivation; stem; tool

DESCRIPTION (provided by applicant): This proposal requests funds for an Olympus FV1000 laser scanning confocal microscope. This system will be housed in the R.M Bock Laboratories at the University of Wisconsin at Madison and be available for members of the three research entities of this building, the Institute of Molecular Virology (IMV), the Laboratory of Molecular Biology (LMB), and the Laboratory for Optical and Computational Instrumentation (LOCI). This instrument will be a valuable tool to investigators who study cell biology and develop novel cellular engineering approaches for stem, neuro, virus, and cancer applications. This confocal will be used for both conventional confocal applications such as multi-label fluorescence studies in 2D and 3D to more advanced applications such as photoactivation, fluorescence recovery after photobleaching (FRAP), and fluorescence resonance energy transfer (FRET). The availability of this modern research tool on site will facilitate existing projects with better equipment access and enable new studies revealing critical new information about cellular processes. )

描述（由申请人提供）：该提案要求为Olympus FV1000激光扫描共聚焦显微镜提供资金。该系统将安置在威斯康星大学麦迪逊分校的R.M Bock实验室中，可用于该建筑物的三个研究实体的成员，分子病毒学研究所（IMV），分子生物学实验室（LMB）和光学和计算仪器实验室（LMB）。对于研究细胞生物学并开发用于STEM，NEURO，病毒和癌症应用的新型细胞工程方法的研究者来说，该仪器将是一种有价值的工具。该共聚焦将用于两种常规的共聚焦应用，例如在2D和3D中进行多标签荧光研究，例如光激活，光漂白后的荧光回收（FRAP）（FRAP）和荧光共振能量转移（FRET）。该现代研究工具在现场的可用性将促进现有的项目，并启用新的研究，并揭示有关蜂窝过程的关键新信息。 ）