Acquisition of a Confocal Microscope for R.M Bock Laboratories

为 R.M Bock 实验室购买共焦显微镜

• 批准号: 7794338
• 负责人: Kevin William Eliceiri
• 金额: $ 39.22万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-22 至 2011-07-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794338
• 关键词: Cell physiology; Cellular biology; Equipment; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Funding; Housing; Institutes; Label; Laboratories; Lasers; Malignant Neoplasms; Microscope; Molecular Biology; Molecular Virology; Optics; Request for Proposals; Research; Research Personnel; Scanning; Site; System; Universities; Virus; Wisconsin; cellular engineering; instrument; instrumentation; member; novel; photoactivation; stem; tool

DESCRIPTION (provided by applicant): This proposal requests funds for an Olympus FV1000 laser scanning confocal microscope. This system will be housed in the R.M Bock Laboratories at the University of Wisconsin at Madison and be available for members of the three research entities of this building, the Institute of Molecular Virology (IMV), the Laboratory of Molecular Biology (LMB), and the Laboratory for Optical and Computational Instrumentation (LOCI). This instrument will be a valuable tool to investigators who study cell biology and develop novel cellular engineering approaches for stem, neuro, virus, and cancer applications. This confocal will be used for both conventional confocal applications such as multi-label fluorescence studies in 2D and 3D to more advanced applications such as photoactivation, fluorescence recovery after photobleaching (FRAP), and fluorescence resonance energy transfer (FRET). The availability of this modern research tool on site will facilitate existing projects with better equipment access and enable new studies revealing critical new information about cellular processes. )

描述（由申请人提供）：该提案要求为奥林巴斯 FV1000 激光扫描共焦显微镜提供资金。该系统将位于威斯康星大学麦迪逊分校的 R.M Bock 实验室内，可供该大楼三个研究实体的成员使用：分子病毒学研究所 (IMV)、分子生物学实验室 (LMB) 和光学和计算仪器实验室（LOCI）。对于研究细胞生物学和开发干细胞、神经、病毒和癌症应用的新型细胞工程方法的研究人员来说，该仪器将是一个有价值的工具。该共焦将用于传统共焦应用（例如 2D 和 3D 中的多标记荧光研究）以及更高级的应用（例如光活化、光漂白后荧光恢复 (FRAP) 和荧光共振能量转移 (FRET)）。这种现代研究工具在现场的可用性将促进现有项目更好地访问设备，并使新研究能够揭示有关细胞过程的关键新信息。 ）