MBF Bioscience and Zeiss Microscope System for Stereology and Tissue Morphology

MBF Bioscience 和蔡司显微镜系统用于体视学和组织形态学

• 批准号: 7794091
• 负责人: Mark Douglas Okusa
• 金额: $ 27.83万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794091
• 关键词: Acute; Advisory Committees; Area; Arts; Autoimmune Diseases; Calendar; Cardiovascular Diseases; Chronic Kidney Failure; Clinical Research; Communication; Computer software; Data; Development; Ensure; Equipment; Faculty; Funding; Funding Applicant; Future; Housing; Hybrids; Image; Image Analysis; Immunity; Individual; Inflammation; Lung diseases; Magnetic Resonance Imaging; Maintenance; Medicine; Methods; Microscope; Microscopy; Minor; Morphology; Nephrology; Online Systems; Recording of previous events; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Resources; Science; Staging; System; Techniques; Technology; Three-dimensional analysis; Time; Tissues; Training; Translations; Ultrasonography; United States National Institutes of Health; Virginia; experience; improved; instrument; instrumentation; medical schools; member; reconstruction; rosin

DESCRIPTION (provided by applicant): Funds are requested to purchase a MBF Bioscience Microscope System for Stereology and Tissue Morphology, including a Zeiss Axio Imager Z1 Microscope with motorized XYZ stage. This powerful hybrid technology combines microscopy, image analysis and stereological tissue morphometrics in one platform. Acquisition of this sophisticated and versatile instrument will provide a new range of tissue imaging and analysis technology to a broad group of investigators whose needs have outgrown the capabilities of our existing instrumentation. This collaborative research group includes investigators in 3 divisions and one Center of the Dept. of Medicine and 2 additional departments of the Univ. of Virginia School of Medicine. The instrument will be housed in a newly renovated centralized shared facility within the Nephrology Division and the Center for Immunity, Inflammation and Regenerative Medicine (CIIR) in the Dept. of Medicine. An advisory committee of 5 senior faculty chaired by the principle investigator (Dr. Mark Okusa, Chief of Nephrology and Director of the CIIR) will oversee the use and maintenance of the instrument and ensure that it is used to its full capacity. Users will reserve instrument time on a Web-based calendar. Two other MBF Bioscience Systems at UVA, located either within individual labs or in shared facilities similar to the one we propose, are unavailable to additional users owing to their heavy usage. A faculty member and member of the CIIR (Dr. Diane Rosin) with extensive experience (15 yrs) in the use of this equipment will serve as Technical Director, train new users, maintain the proposed facility, and facilitate communication with the highly accessible support team at MBF Bioscience. MBF has provided outstanding support worldwide for 20 years by offering timely and responsive trouble-shooting and serving as an enthusiast resource of ongoing development (often user- generated) of modified analysis techniques for its users. The 5 NIH-funded Major Users and 6 Minor Users in this proposal have a strong history of NIH funding and a high potential for future benefit from the scientific impact of the acquisition of this instrument. For state-of-the-art advanced imaging and stereological analysis technology, the specific features found only in the requested instrument are vital for the diverse array of projects described by the Users. The instrument is highly suited to a multi-user setting because integration of the motorized microscope stage by different software modules provides a wide array of data capture and analysis methods (for example, 2D or 3D analysis and reconstruction), data can either be acquired online at the workstation or imported from a wide array of other image applications (MRI, EM, confocal, ultrasound), and users can store personalized settings. With the vast potential of this instrument for use in basic, translation and clinical research projects, the studies that will be enhanced by this instrument will improve our understanding of a wide variety of areas in biomedical sciences, including acute and chronic kidney diseases, autoimmune diseases, inflammation and immunity, fibroproliferative diseases, and cardiovascular and pulmonary diseases.

描述（由申请人提供）： 需要资金购买用于体视学和组织形态学的 MBF 生物科学显微镜系统，包括带有电动 XYZ 载物台的 Zeiss Axio Imager Z1 显微镜。这一强大的混合技术将显微镜、图像分析和体视组织形态测量结合在一个平台上。购买这种复杂且多功能的仪器将为广大研究人员提供一系列新的组织成像和分析技术，这些研究人员的需求已经超出了我们现有仪器的能力。该合作研究小组包括来自医学系 3 个部门和一个中心以及大学另外 2 个部门的研究人员。弗吉尼亚医学院。该仪器将安置在肾脏科和医学系免疫、炎症和再生医学中心 (CIIR) 内新装修的集中共享设施中。由首席研究员（肾脏病科主任兼 CIIR 主任 Mark Okusa 博士）担任主席的 5 名高级教职人员组成的咨询委员会将监督该仪器的使用和维护，并确保其得到充分利用。用户将在基于网络的日历上预留仪器时间。 UVA 的另外两个 MBF 生物科学系统位于单独的实验室内或类似于我们建议的共享设施中，由于使用率较高，其他用户无法使用。一位在使用该设备方面拥有丰富经验（15 年）的 CIIR 教员和成员（Diane Rosin 博士）将担任技术总监，培训新用户，维护拟议的设施，并促进与便捷支持人员的沟通MBF Bioscience 团队。 20 年来，MBF 在全球范围内提供了出色的支持，提供及时、响应迅速的故障排除，并作为为其用户不断开发（通常是用户生成的）改进分析技术的爱好者资源。本提案中的 5 个 NIH 资助的主要用户和 6 个次要用户拥有 NIH 资助的悠久历史，并且未来很有可能从购买该仪器的科学影响中受益。对于最先进的先进成像和体视分析技术，仅在所需仪器中找到的特定功能对于用户描述的各种项目至关重要。该仪器非常适合多用户设置，因为通过不同的软件模块集成电动显微镜载物台提供了多种数据捕获和分析方法（例如，2D 或 3D 分析和重建），数据可以在线获取在工作站上或从各种其他图像应用程序（MRI、EM、共聚焦、超声）导入，用户可以存储个性化设置。该仪器在基础、转化和临床研究项目中具有巨大的应用潜力，该仪器将增强的研究将提高我们对生物医学科学各个领域的理解，包括急性和慢性肾脏疾病、自身免疫性疾病、炎症和免疫、纤维增生性疾病、心血管和肺部疾病。