MBF Bioscience and Zeiss Microscope System for Stereology and Tissue Morphology

MBF Bioscience 和蔡司显微镜系统用于体视学和组织形态学

• 批准号: 7794091
• 负责人: Mark Douglas Okusa
• 金额: $ 27.83万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794091
• 关键词: Acute; Advisory Committees; Area; Arts; Autoimmune Diseases; Calendar; Cardiovascular Diseases; Chronic Kidney Failure; Clinical Research; Communication; Computer software; Data; Development; Ensure; Equipment; Faculty; Funding; Funding Applicant; Future; Housing; Hybrids; Image; Image Analysis; Immunity; Individual; Inflammation; Lung diseases; Magnetic Resonance Imaging; Maintenance; Medicine; Methods; Microscope; Microscopy; Minor; Morphology; Nephrology; Online Systems; Recording of previous events; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Resources; Science; Staging; System; Techniques; Technology; Three-dimensional analysis; Time; Tissues; Training; Translations; Ultrasonography; United States National Institutes of Health; Virginia; experience; improved; instrument; instrumentation; medical schools; member; reconstruction; rosin

DESCRIPTION (provided by applicant): Funds are requested to purchase a MBF Bioscience Microscope System for Stereology and Tissue Morphology, including a Zeiss Axio Imager Z1 Microscope with motorized XYZ stage. This powerful hybrid technology combines microscopy, image analysis and stereological tissue morphometrics in one platform. Acquisition of this sophisticated and versatile instrument will provide a new range of tissue imaging and analysis technology to a broad group of investigators whose needs have outgrown the capabilities of our existing instrumentation. This collaborative research group includes investigators in 3 divisions and one Center of the Dept. of Medicine and 2 additional departments of the Univ. of Virginia School of Medicine. The instrument will be housed in a newly renovated centralized shared facility within the Nephrology Division and the Center for Immunity, Inflammation and Regenerative Medicine (CIIR) in the Dept. of Medicine. An advisory committee of 5 senior faculty chaired by the principle investigator (Dr. Mark Okusa, Chief of Nephrology and Director of the CIIR) will oversee the use and maintenance of the instrument and ensure that it is used to its full capacity. Users will reserve instrument time on a Web-based calendar. Two other MBF Bioscience Systems at UVA, located either within individual labs or in shared facilities similar to the one we propose, are unavailable to additional users owing to their heavy usage. A faculty member and member of the CIIR (Dr. Diane Rosin) with extensive experience (15 yrs) in the use of this equipment will serve as Technical Director, train new users, maintain the proposed facility, and facilitate communication with the highly accessible support team at MBF Bioscience. MBF has provided outstanding support worldwide for 20 years by offering timely and responsive trouble-shooting and serving as an enthusiast resource of ongoing development (often user- generated) of modified analysis techniques for its users. The 5 NIH-funded Major Users and 6 Minor Users in this proposal have a strong history of NIH funding and a high potential for future benefit from the scientific impact of the acquisition of this instrument. For state-of-the-art advanced imaging and stereological analysis technology, the specific features found only in the requested instrument are vital for the diverse array of projects described by the Users. The instrument is highly suited to a multi-user setting because integration of the motorized microscope stage by different software modules provides a wide array of data capture and analysis methods (for example, 2D or 3D analysis and reconstruction), data can either be acquired online at the workstation or imported from a wide array of other image applications (MRI, EM, confocal, ultrasound), and users can store personalized settings. With the vast potential of this instrument for use in basic, translation and clinical research projects, the studies that will be enhanced by this instrument will improve our understanding of a wide variety of areas in biomedical sciences, including acute and chronic kidney diseases, autoimmune diseases, inflammation and immunity, fibroproliferative diseases, and cardiovascular and pulmonary diseases.

描述（由申请人提供）：要求资金购买用于立体和组织形态的MBF生物科学显微镜系统，包括具有电动XYZ阶段的Zeiss Axio Imager Z1显微镜。这种强大的混合技术结合了一个平台中的显微镜，图像分析和立体组织形态计量学。获得这种复杂且通用的仪器的获取将为一群研究人员提供新的组织成像和分析技术，这些研究人员的需求已经超过了我们现有仪器的能力。这个合作研究小组包括三个部门和一个医学系中心的调查人员和大学的其他两个部门。弗吉尼亚医学院。该仪器将安置在肾脏科部门和医学系的免疫，炎症和再生医学中心（CIIR）的新近翻新的集中共享设施中。由主要研究人员主持的5位高级教职员工（肾脏科负责人兼CIIR主任Mark Okusa博士）的咨询委员会将监督该工具的使用和维护，并确保其全部使用。用户将在基于Web的日历上保留仪器时间。 UVA的其他两个MBF Bioscience系统位于单个实验室内或类似于我们建议的设施中的共享设施中，由于其大量使用而无法获得其他用户。在使用本设备方面的丰富经验（15年）的CIIR（Diane Rosin博士）的教职员工和成员将担任技术主管，培训新用户，维护拟议的设施，并促进与MBF Bioscience的高度可访问的支持团队的沟通。 MBF通过提供及时且响应迅速的故障射击，并作为对用户的修改分析技术的持续开发（通常是用户生成）的发烧友资源，提供了20年的出色支持。该提案中的5个由NIH资助的主要用户和6名未成年人使用了NIH资金的悠久历史，并且从收购该工具的科学影响中获得了未来的利益，从而具有很高的潜力。对于最先进的高级成像和立体分析技术，仅在请求的仪器中发现的具体功能对于用户描述的各种项目至关重要。 The instrument is highly suited to a multi-user setting because integration of the motorized microscope stage by different software modules provides a wide array of data capture and analysis methods (for example, 2D or 3D analysis and reconstruction), data can either be acquired online at the workstation or imported from a wide array of other image applications (MRI, EM, confocal, ultrasound), and users can store personalized settings.凭借该工具用于基本，翻译和临床研究项目的巨大潜力，该工具将增强的研究将提高我们对生物医学科学领域的各种领域的理解，包括急性和慢性肾脏疾病，自身免疫性疾病，炎症和免疫，纤维化疾病，以及心脏病和心脏疾病。