ID of Biomarkers in Exhaled Breath Condensates from Asthmatic Patients

哮喘患者呼出气体冷凝物中生物标志物的识别

• 批准号: 7969044
• 负责人: Stewart Levine
• 金额: $ 41.9万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7969044
• 关键词: Age; Asthma; Biological Markers; Collection; Detection; Devices; Disease; Engineering; Enrollment; Exhalation; Female; Goals; Individual; Laboratories; Mass Spectrum Analysis; Methods; Morbidity - disease rate; National Heart, Lung, and Blood Institute; Patients; Physics; Protocols documentation; Public Health; Refractory; Research Ethics Committees; Risk; Sampling; Specimen; Tube; Universities; airway inflammation; asthmatic patient; disorder control; male; mortality; novel; prevent; volunteer

The aim of this exploratory specimen procurement protocol is to develop a non-invasive method for the detection of asthma-specific biomarkers in exhaled breath condensates (EBC) utilizing a novel collection device that was engineered at the Johns Hopkins University Applied Physics Laboratory. The goal of the study is to identify biomarkers in samples from 3 groups; (i) non-asthmatic normal volunteers, (ii) asthmatic patients whose disease is classified as intermittent or mild, persistent, and (iii) asthmatic patients whose disease is classified as moderate or severe persistent. Each group will consist of 24 patients. These individuals must be between the ages of 18 and 75 and can be male or female. Samples of exhaled breath condensate will be collected using the JHU APL EBC collection device, as well as with the R-Tube. Biomarkers in EBC will be identified by mass spectroscopy. We propose that the identification of biomarkers in EBC that correlate with airway inflammation and disease activity may be used to guide therapy and prevent exacerbations in asthmatic patients. This study was approved by the NHLBI IRB on 2/5/08 and no subjects have been enrolled to date.

该探索性标本采购方案的目的是开发一种非侵入性方法，用于在呼出的呼吸冷凝物中检测哮喘特异性的生物标志物（EBC），该方法利用约翰·霍普金斯大学（Johns Hopkins University）设计的新型收集装置（EBC）使用新颖的收集设备。 该研究的目的是识别来自3组样品的生物标志物； （i）非雅典正常志愿者，（ii）疾病被归类为间歇性或轻度，持久性和（iii）疾病的哮喘患者，其疾病被归类为中等或严重的持久性。 每个组将由24名患者组成。 这些人必须年龄在18至75岁之间，并且可以是男性或女性。 将使用JHU APL EBC收集设备以及R-Tube收集呼气的呼吸冷凝物样品。 EBC中的生物标志物将通过质谱鉴定。 我们建议，与气道炎症和疾病活动相关的EBC中生物标志物的鉴定可用于指导治疗并防止哮喘患者加重。 这项研究已由NHLBI IRB于2008年2月5日批准，迄今为止尚未招募任何受试者。