Cellular Basis Of Action Of Gastrointestinal Peptides/Growth factors

胃肠肽/生长因子作用的细胞基础

• 批准号: 7967526
• 负责人: Robert Jensen
• 金额: $ 45.74万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7967526
• 关键词: Acinus organ component; Area; Bombesin Receptor; Cell physiology; Cells; Cholecystokinin Receptor; Gastrointestinal Hormones; Gastrointestinal tract structure; Goals; Growth; Growth Factor; Malignant neoplasm of lung; Modeling; Neuraxis; Non-Steroidal Anti-Inflammatory Agents; Pancreas; Peptides; Phosphorylation; Process; Protein Tyrosine Kinase; Proto-Oncogene Proteins c-akt; Receptor Activation; Reporting; Signal Transduction; Sulfur; System; Tissues; Tyrosine Phosphorylation; abstracting; base; cancer cell; cell growth; cell motility; cytotoxic; gastrointestinal; neoplastic; novel

RRecent studies show that gastrointestinal hormones/growth factors may stimulate cell growth by stimulating multiple intracellular tyrosine phosphorylation (TyrP) signaling cascades. However at present little is known about the ability of many gastrointestinal hormones/growth factors to activate these cascades in GI tissues. Our studies have been in two general areas, which include studies of intracellular signaling cascades primarily by tyrosine kinases and studies of tumoral growth attempting to develop novel agents for growth inhibition. Recent studies show that gastrointestinal hormones, similar to growth factors, may stimulate cell growth/cell signaling by stimulating multiple intracellular tyrosine phosphorylation (TyrP) cascades. Whereas these cascades have been extensively investigated with growth factors, little is known in this area with may gastrointestinal hormones. The goal of these studies is to clarify this area primarily concentrating on cholecystokinin receptor cascades and bombesin receptor activation using primarily pancreatic acini as a model natural cell system. Studies involving phosphorylation of PKD1 and GAB1 have been recently completed and during this year a detailed study of the ability of gastrointestinal hormones and growth factors to activate AKT was completed. Studies of PKC theta activation were reported in abstract form and will be completed later this year. The ability of novel cytotoxic sulfur NSAID to alter growth of lung cancer cells was also studied this year.

最近的研究表明，胃肠激素/生长因子可能通过刺激多个细胞内酪氨酸磷酸化 (TyrP) 信号级联来刺激细胞生长。然而，目前人们对许多胃肠激素/生长因子激活胃肠道组织中这些级联反应的能力知之甚少。 我们的研究主要集中在两个领域，其中包括主要通过酪氨酸激酶进行的细胞内信号级联的研究以及试图开发新的生长抑制药物的肿瘤生长的研究。最近的研究表明，胃肠激素与生长因子类似，可以通过刺激多个细胞内酪氨酸磷酸化（TyrP）级联来刺激细胞生长/细胞信号传导。虽然这些级联反应已通过生长因子进行了广泛的研究，但在这一领域对胃肠激素的了解却很少。这些研究的目的是阐明这一领域，主要集中于胆囊收缩素受体级联和铃蟾肽受体激活，主要使用胰腺腺泡作为自然细胞系统模型。涉及 PKD1 和 GAB1 磷酸化的研究最近已完成，今年还完成了胃肠激素和生长因子激活 AKT 能力的详细研究。 PKC theta 激活的研究以摘要形式报告，将于今年晚些时候完成。今年还研究了新型细胞毒性硫非甾体抗炎药改变肺癌细胞生长的能力。