Absolute Quantification of Molecular Representation and Interaction

分子表示和相互作用的绝对定量

• 批准号: 7964731
• 负责人: Aleksandra Nita-Lazar
• 金额: $ 16.68万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7964731
• 关键词: Biological; Collaborations; Data; Equipment; Freedom; Housing; Human Resources; Immune system; Laboratories; Mass Spectrum Analysis; Methodology; Methods; Modeling; Molecular; Pathway interactions; Peptides; Proteins; Reagent; Relative (related person); Signal Transduction; System; Testing; base; design; instrument; mass spectrometer; mathematical model; nano; predictive modeling; protein expression; quantum; research study

The group began in April 2009 and the projects were assigned in July 2009. We are organizing the laboratory, hiring personnel and designing experiments. The equipment and reagents are being purchased and the laboratory space adjusted for housing the mass spectrometers. We have purchased the LTQ-Orbitrap and TSQ Quantum Vantage from Thermo Fisher, and the nano-LC systems. These instruments should cover the range of initially planned experiments. We are designing the methods for absolute quantification, developing and testing standards of tagged proteins (in collaboration with Dr. Iain Fraser) and designing peptides for AQUA method of MS-based quantification.

该小组始于2009年4月，这些项目于2009年7月分配。 我们正在组织实验室，招聘人员和设计实验。 正在购买设备和试剂，并调整了用于容纳质谱仪的实验室空间。我们从Thermo Fisher和Nano-LC系统购买了LTQ-Orbitrap和TSQ量子Vantage。这些仪器应涵盖最初计划的实验范围。我们正在设计用于绝对量化，制定和测试标记蛋白（与Iain Fraser博士合作的）的方法，并为基于MS的定量化的Aqua方法设计肽。