Vector Shared Resource

矢量共享资源

• 批准号: 7944613
• 负责人: NORIYUKI KASAHARA
• 金额: $ 11.59万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-02 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944613
• 关键词: Adenovirus Vector; Animal Experiments; Applications Grants; Area; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cellular biology; Characteristics; Consultations; Custom; Development; Disease; Dominant-Negative Mutation; Epitopes; Gene Delivery; Gene Expression Regulation; Gene Transfer; Genes; Genetic; Genitourinary system; Hematopoietic Neoplasms; In Vitro; Individual; Jonsson Comprehensive Cancer Center of University of California Los Angeles; Laboratories; Libraries; Malignant - descriptor; Malignant Neoplasms; Mammalian Cell; Methodology; Molecular Epidemiology of Cancer; Organogenesis; Pathogenesis; Preparation; Production; Recording of previous events; Reporting; Research; Research Activity; Research Personnel; Resource Sharing; Resources; Services; Signal Transduction; Source; Techniques; Technology; Therapeutic; Thoracic Oncology; Transgenes; Translational Research; Viral Vector; Woman; Work; anticancer research; cancer cell; cellular engineering; cost; design and construction; experience; gene therapy; in vivo; interest; meetings; member; molecular imaging; mutant; new therapeutic target; oncology; programs; research study; tumor immunology; vector

The Vector Shared Resource (VSR). Advances in techniques for efficient cellular engineering using virusbased gene delivery vehicles ("vectors") have made it feasible to utilize gene transfer as a methodology for elucidating functions of specific genes, by examining consequences of their over-expression or inhibition. The Vector Shared Resource is a new Shared Resource, established to meet the increasing demand both for routine construction and production of viral vectors and for assistance in developing new vectors, both for cell culture and in vivo animal experiments. The objective of the Vector Shared Resource is to promote and Facilitate basic and translational research by providing JCCC investigators with access to vector technologies that enable efficient gene transfer to mammalian cells, both in culture and in vivo. Our services include: (1) provision, at minimal cost, of various pre-made retroviral, lentiviral and adenoviral vector stocks expressing standard marker genes to utilize in preliminary experiments, as well as a library of available vectors expressing various mammalian genes and corresponding inhibitory sequences; (2) construction and production of custom viral vectors that contain a specific sequence(s) of interest (including wild type and mutant cDNAs with or without epitope tags, dominant-negative expression constructs, antisense mRNAs, siRNAs, etc.) for individual researchers; and (3) provision of educational and advisory resources for researchers with limited experience in viral vector technologies, but who wish to utilize such technologies for efficient functional expression of genetic sequences of interest in vitro and/or in vivo. As the use of viral vector technology requires specialized expertise and resources often not found in an individual investigator's laboratory, offering easy access to these technologies, consolidated in the form of a Shared Resource, can significantly facilitate and expand the scope of JCCC research activities. Furthermore, our services are more cost-effective than utilizing the limited commercial sources offering such technologies. Further value is added by customized technical support available from accessible and knowledgeable Shared Resource staff who can work closely with investigators to troubleshoot and optimize gene transfer experiments, assist with institutional regulatory compliance documents and grant proposal submissions and who are actively engaged in development and application of new vector technologies. Initiated in 2003, the Vector Shared Resource has been used by members of the Signal Transduction and Therapeutics, Women's Cancer, Hematopoietic Malignancies, Gene Regulation, Cancer Cell Biology, Tumor Immunology, Thoracic Oncology, Genitourinary Oncology, Molecular Epidemiology and Cancer Molecular Imaging Program Areas for consultation, vector construction, small and large scale preparation of vector stocks and additional services. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). 25 Cancer Center members representing 10 Cancer Center Program Areas utilized the services of the Vector Shared Resource during the reporting period. This is a new shared resource.

向量共享资源（VSR）。使用基于病毒的有效蜂窝工程技术的进步 基因输送车（“向量”）使使用基因转移作为一种方法 通过检查其过表达或抑制作用的后果来阐明特定基因的功能。 向量共享资源是一种新的共享资源，旨在满足不断增长的需求 用于常规建设和生产病毒媒介，并为开发新向量提供帮助，均用于 细胞培养和体内动物实验。向量共享资源的目的是促进和 通过为JCCC调查人员提供访问向量技术来促进基础和转化研究 在培养和体内，这使得有效的基因转移到了哺乳动物细胞。我们的服务包括：（1） 以最小的成本提供各种预制逆转录病毒，慢病毒和腺病毒矢量库存的供应 标准标记基因在初步实验中使用，以及可用向量的库 表达各种哺乳动物基因和相应的抑制序列； （2）施工和 生产包含特定序列的定制病毒载体（包括野生类型和 带有或不具有表位标签的突变cDNA，主要阴性表达构建体，反义mRNA， sirnas等）对于个别研究人员； （3）为教育和咨询资源提供 在病毒矢量技术方面经验有限的研究人员，但希望利用此类技术 体外和/或体内感兴趣的遗传序列的有效功能表达。作为病毒的使用 向量技术需要专业的专业知识和资源，通常在个人调查员的 实验室，可轻松访问这些技术，以共享资源的形式合并，可以 显着促进和扩大JCCC研究活动的范围。此外，我们的服务是 比利用有限的商业资源提供此类技术更具成本效益。进一步的价值是 由可访问且知识渊博的共享资源人员提供的定制技术支持添加 谁可以与研究人员紧密合作，以进行故障排除和优化基因转移实验，协助 机构监管合规文件和赠款提案提交以及谁是积极的 从事新向量技术的开发和应用。矢量于2003年发起，共享 信号转导和治疗剂，女性癌症的成员已使用了资源 造血恶性肿瘤，基因调节，癌细胞生物学，肿瘤免疫学，胸腔 肿瘤学，生殖肿瘤学，分子流行病学和癌症分子成像程序区域 进行咨询，矢量建设，矢量库存的小规模和大规模准备以及其他 服务。 （另请参见有关历史记录，描述，必不可少的共享资源的第6.2.3节 特征）。 25个代表10个癌症中心计划领域的癌症中心成员利用了 向量共享资源在报告期间。这是一个新的共享资源。