COBRE: U OF KANSAS MEDICAL CTR: CORE C: NULL-MOUSE CORE

COBRE：堪萨斯大学医学中心 CTR：核心 C：空鼠标核心

• 批准号: 7959504
• 负责人: GRACE L GUO
• 金额: $ 3.92万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959504
• 关键词: Animals; Breeding; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Disease; Engineering; Funding; Genotype; Grant; Health; Hepatocyte; Income; Individual; Institution; Kansas; Knock-out; Knockout Mice; Liver; Maintenance; Medical; Mentors; Mus; Nuclear Receptors; Quarantine; Research; Research Personnel; Research Project Grants; Resources; Role; Source; Time; United States National Institutes of Health; material transfer agreement; mouse model; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Null-Mouse Core will maintain standing colonies of nuclear-receptor null-mice to serve the scientific needs of the five individual research projects, as well as those of the mentors and other investigators at the institution. Because numerous investigators will require nuclear-receptor null-mice, it is much more efficient and costeffective to have a "bank" of null-mice. The intent is to have 5 breeding pairs of each null strain in the nullmouse core. When an investigator needs to use a specific null mouse, three pairs of breeders will be transferred to the investigator, and the investigator will start paying the mouse maintenance. This will also save time because, MTAs (material transfer agreements) won't need to be obtained, nor income animals quarantine time. At the present time, we have the following null colonies: PXR, AhR, RXRa, CAR, PPARa, Nr'2, HNF1a, FXR (whole-body and hepatocyte-specific) and hepatocyte-specific PPARy and HNF4a. For the projects of this proposal, SHP-null mice will be obtained from David Moore at Baylor (Dr Wang's mentor). In addition, Core C will have the technical capability to engineer other new knockouts or double knockouts as needed. Another feature of this Core will be to conduct routine genotyping experiments necessary to demonstrate the ongoing integrity of the null-mouse model, as well as to engineer double-null mice, and perform the genotyping. The other major role of this core is to isolate liver, prepare hepatocytes, and perform culture experiments upon request for the investigators of this COBRE.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 零鼠核心将维持核受体无效的殖民地，以满足五个单独的研究项目的科学需求以及该机构的导师和其他研究人员的科学需求。 由于许多调查人员需要核受体无效，因此拥有“无效的银行”的效率和成本效益要高得多。目的是在零群核中有5对每个零菌株的繁殖对。当研究人员需要使用特定的空小鼠时，将将三对育种者转移到研究人员中，研究人员将开始支付小鼠的维护。这也将节省时间，因为MTA（物质转移协议）无需获得，也不需要获得收入动物隔离时间。目前，我们有以下零菌落：PXR，AHR，RXRA，CAR，PPARA，NR'2，HNF1A，FXR（全身和肝细胞特异性）和肝细胞特异性PPARY PPARY和HNF4A。对于该提案的项目，SHP-Null小鼠将从贝勒（Wang's Mentor博士）的David Moore获得。此外，Core C将具有技术能力，可以根据需要设计其他新的淘汰赛或双重淘汰赛。 该核心的另一个特征是进行常规的基因分型实验，以证明Null Mouse模型的持续完整性以及工程师的双重无效小鼠并执行基因分型。该核心的另一个主要作用是分离肝脏，制备肝细胞并应要求该毛病的研究人员进行培养实验。