ZEISS/31 STALLION DDI MULTI-DIMENSIONAL LIVE-CELL IMAGING WORKSTATION

ZEISS/31 STALLION DDI 多维活细胞成像工作站

• 批准号: 7335269
• 负责人: J. Michael MCCAFFERY
• 金额: $ 30.35万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335269
• 关键词: aging; apoptosis; biotechnology; cell adhesion; centromere; complement; digital imaging; endocytosis; fasting; fluorescence; genes; lasers; lysosomes; medicine; membrane biogenesis; microscopy; mitochondria; neoplasm /cancer; particle; photoactivation; public health; schools; telomere; university; vesicle /vacuole

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health; and the University of Maryland Biotechnology Institute in the University of Maryland School of Medicine request funds to purchase a Stallion DPI Live-Cell digital Imaging workstation from Carl Zeiss/lntelligent Imaging Innovations. The requested system integrates a Zeiss 200M TIRF-ready platform with dual ultra-fast/highly sensitive Cascade 512B EM digital cameras; a 405/488 FRAP/photoactivation laser module; fully integrated 31 Slidebook multidimensional acquisition/analysis software; and is optimized for 1) rapid/ultra-sensitive 4D two channel acquisition; 2) deconvolution; 3) FRET and ratio imaging; 4) particle tracking; and 5) FRAP and photoactivation. The new state-of-the-art workstation will complement our heavily utilized Zeiss LSM 510 METAs and aging Deltavision system, while constituting a significant upgrade in speed and sensitivity. Importantly, the Stallion DPI will provide new/unique widefield fluorescence capabilities for the Hopkins' Integrated Imaging Center, a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: telomere/centromere reorganization in tumors; HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. In the requested configuration, the Stallion DDI workstation will afford our disparate group of investigators a broad range of basic/advanced live-cell microscopy capabilities that are presently deficient/absent from the Hopkins Homewood campus. The new system will be conveniently located, administered, and maintained, in the Biology Department's Integrated Imaging Center (NO: and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：由 11 名 NIH 资助的研究人员组成的多元化小组，隶属于约翰·霍普金斯大学艺术与科学学院、工程学院、医学学院和公共卫生学院；马里兰大学医学院和马里兰大学生物技术研究所请求资金从 Carl Zeiss/intelligent Imaging Innovations 购买 Stallion DPI Live-Cell 数字成像工作站。所要求的系统集成了 Zeiss 200M TIRF 就绪平台和双超快/高灵敏度 Cascade 512B EM 数码相机； 405/488 FRAP/光激活激光模块；完全集成的31 Slidebook多维采集/分析软件；并针对 1) 快速/超灵敏 4D 双通道采集进行了优化； 2）反卷积； 3）FRET和比率成像； 4）粒子追踪； 5) FRAP 和光活化。新的最先进的工作站将补充我们大量使用的 Zeiss LSM 510 META 和老化的 Deltavision 系统，同时在速度和灵敏度方面实现重大升级。重要的是，Stallion DPI 将为霍普金斯大学的综合成像中心提供新的/独特的宽场荧光功能，该中心是霍姆伍德校园/霍普金斯大学范围内的显微镜资源，由多个学校和部门定期使用，包括超过 60 个实验室和约 200 个用户。我们的研究人员均通过 NIH 获得充足的资助，致力于研究一系列基本的细胞生物学、生化和生物分子相关问题，包括但不限于：肿瘤中的端粒/着丝粒重组； HSV 组装和成熟； MHC I 运输和组装、膜组织和动力学；内体/溶酶体动力学；膜贩运；贩运聚合基因载体；线粒体和细胞凋亡；唾液酸展示和细胞粘附；和内吞作用的机制。在所要求的配置中，Stallion DDI 工作站将为我们不同的研究人员小组提供广泛的基本/高级活细胞显微镜功能，这些功能目前在霍普金斯霍姆伍德校园中缺乏/缺乏。新系统将在生物系综合成像中心（NO：NO：）中方便地定位、管理和维护，并将并入IIC现有的、完善的充值系统，以确保回收供应和维护资金。