SMALL ANIMAL ULTRASOUND IMAGER - VEVO 770: CARDIOVASCULAR

小动物超声成像仪 - VEVO 770：心血管

• 批准号: 7335138
• 负责人: CARLIN S LONG
• 金额: $ 13.24万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335138
• 关键词: kidney; measurement; model; physiology; ultrasound

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The recent explosion in the availability of defined genetic rodent models, mostly murine but also rat, has led to considerable advances in molecular physiology. The challenge with rodent physiology models is one of scale - how to perform precise in vivo measurements that yield quantitative and meaningful data. This proposal requests funding for a state-of-the-art Small Animal Ultrasound Micro-lmager - the VisualSonics Vevo 770. The instrument will be managed and operated by the Small Animal Hemodynamic Core Facility within the Division of Cardiology at UCHSC. The instrument will be used by an interdisciplinary group of NIH-funded investigators within the University of Colorado campuses. Eleven major users of the instrument are located on the Health Sciences Center campus and one investigator is located on the Boulder campus. Several minor users of the instrument are also located on these campuses. This group of investigators has an extensive history of productive collaborations. Research projects benefiting from the acquisition of this instrument can be broadly defined as follows: (1) rodent models of heart failure; (2) pulmonary hypertension and right ventricular function; (3) renal regulation of hemodynamics; and (4) therapeutic applications of stem cells. Ultrasound images are currently being acquired with a 6-year-old VingMed clinical echocardiographic machine equipped with a 10 MHz linear array probe. The Vevo 770, which has a resolution of 30-70um, is an ultrasound instrument developed for use in small animals. Purchase of this instrument will greatly extend the ultrasound capabilities available to the Core users and ensure that future in vivo imaging needs will be met for years to come. Anticipated uses for the Vevo 770 include determination of cardiac wall motion abnormalities and calculation of ejection fraction in mice using 2-D imaging modalities; measurement of cardiac output in mice and rats using Pulse-Wave Doppler; quantitation of right ventricular function by M- mode and 2-D imaging modalities in mice and rats; image guided injection of stem cells into embryonic and adult mice; and renal blood flow in mice and rats. The use of transducers with specific focal lengths and axial resolution enhances the adaptability of this instrument to different research applications. We anticipate that the enthusiasm for use of this instrument will only increase over time as more investigators move to small animal model systems to further define molecular interactions in an in vivo setting.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：最近，确定的遗传啮齿动物模型（主要是小鼠，但也有大鼠）的可用性激增，导致了分子生理学的巨大进步。啮齿动物生理学模型的挑战之一是规模——如何进行精确的体内测量以产生定量且有意义的数据。该提案要求为最先进的小动物超声显微成像仪 - VisualSonics Vevo 770 提供资金。该仪器将由 UHSC 心脏病科内的小动物血流动力学核心设施管理和操作。该仪器将由美国国立卫生研究院资助的科罗拉多大学校园内的跨学科研究小组使用。该仪器的 11 位主要用户位于健康科学中心校区，一名研究人员位于博尔德校区。该仪器的几个小用户也位于这些校园内。这组研究人员有着丰富的富有成效的合作历史。受益于购买该仪器的研究项目可大致定义如下：（1）心力衰竭的啮齿动物模型； (2)肺动脉高压及右心功能； (3)肾脏对血流动力学的调节； (4)干细胞的治疗应用。目前正在使用一台已使用 6 年、配备 10 MHz 线性阵列探头的 VingMed 临床超声心动图机采集超声​​图像。 Vevo 770 的分辨率为 30-70um，是一款专为小动物开发的超声仪器。购买该仪器将大大扩展核心用户可用的超声功能，并确保在未来几年内满足未来的体内成像需求。 Vevo 770 的预期用途包括使用 2D 成像模式确定小鼠心壁运动异常和计算射血分数；使用脉冲波多普勒测量小鼠和大鼠的心输出量；通过 M 模式和 2-D 成像方式对小鼠和大鼠的右心室功能进行定量；图像引导将干细胞注射到胚胎和成年小鼠中；以及小鼠和大鼠的肾血流量。使用具有特定焦距和轴向分辨率的传感器增强了该仪器对不同研究应用的适应性。我们预计，随着越来越多的研究人员转向小动物模型系统以进一步定义体内环境中的分子相互作用，使用该仪器的热情只会随着时间的推移而增加。