PROTEOMIC CHARACTERIZATION OF CEREBROSPINAL FLUID BY HIGH RESOLUTION LC-MS/MS

通过高分辨率 LC-MS/MS 进行脑脊液蛋白质组学表征

• 批准号: 7957005
• 负责人: H Shaw Warren
• 金额: $ 3.25万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957005
• 关键词: Biology; Brain; Brain Diseases; Cerebrospinal Fluid; Clinical Medicine; Computer Retrieval of Information on Scientific Projects Database; Databases; Disease; Funding; Future; Gel; Goals; Grant; Human; Institution; Knowledge; Measures; Monitor; National Center for Research Resources; Proteins; Proteome; Proteomics; Research; Research Personnel; Resolution; Resources; Sampling; Source; Spinal Cord; Techniques; Time; United States National Institutes of Health; base; protein distribution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This NCRR collaborative project has focused on the proteome characterization of human cerebrospinal fluid (CSF). CSF is routinely sampled in clinical medicine to help determine and monitor the presence of illness in the brain or spinal cord. Clinicians usually measure the total protein concentration for this purpose since very little is known about the protein composition, or proteome, of CSF in normal or disease states. Much of the current knowledge about CSF is based upon 2D gel analysis techniques which are severely limited in both sensitivity and throughput. The overall goals of the research proposed in this study are twofold: (1) determine, as possible, the content and distribution of proteins in "normal" human CSF for the creation of an accurate mass and time (AMT) tag database for future quantitative, high sensitivity LC-MS studies; and (2) evaluate how the content and abundance of proteins in CSF are altered in specific diseases of the brain and spinal cord.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该 NCRR 合作项目重点关注人类脑脊液 (CSF) 的蛋白质组表征。 临床医学中通常会采集脑脊液样本，以帮助确定和监测大脑或脊髓是否存在疾病。 临床医生通常为此目的测量总蛋白浓度，因为对正常或疾病状态下脑脊液的蛋白质组成或蛋白质组知之甚少。 目前关于 CSF 的大部分知识都是基于 2D 凝胶分析技术，这些技术在灵敏度和通量方面都受到严重限制。 本研究提出的研究总体目标有两个：（1）尽可能确定“正常”人脑脊液中蛋白质的含量和分布，以创建准确的质量和时间（AMT）标签数据库，用于未来的定量分析，高灵敏度LC-MS研究； (2) 评估脑脊液中蛋白质的含量和丰度在大脑和脊髓的特定疾病中如何改变。