RNA POLYMERASE II-CAPPING ENZYME COMPLEX STRUCTURE

RNA 聚合酶 II 封端酶复合物结构

• 批准号: 7955178
• 负责人: JIANHUA FU
• 金额: $ 0.43万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955178
• 关键词: Biochemical; Cell Cycle; Cell physiology; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Polymerase II; Enzymes; Equilibrium; Funding; Gene Expression; Generations; Genetic Programming; Genetic Transcription; Grant; Hormones; Institution; Malignant Neoplasms; Methods; Multienzyme Complexes; Oncogene Proteins; Phosphoric Monoester Hydrolases; RNA Polymerase II; Repression; Research; Research Personnel; Resources; Ribosomes; Roentgen Rays; Signal Transduction; Source; Stimulus; Structure; Transcriptional Regulation; United States National Institutes of Health; Work; Yeasts; detection of nutrient; mRNA Precursor; response; structural biology; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is the first step in gene expression. Signals from many cellular processes such as nutrient sensing, catabolite repression, hormone stimulation, differentiation and responses to environmental stimuli ultimately reach the level of transcription to exert their effects. Aberration of transcriptional control is one of the mechanisms underlying tumorigenesis, since cancer arises from disruption of the fine balance between proliferative and differentiative genetic programs. Oncogene products are frequently found to be key players in the control networks of signal transduction, cell cycle and transcription regulations. We propose to examine, in yeast, various Pol II-factor(s) complexes corresponding to distinct functional steps in the transcription cycle. Biochemical and X-ray crystallographic methods developed in the work of the 10-subunit Pol II and the ribosome will be employed, refined and extended to tackle this new generation of structural problems in transcription regulation. Specific aims for the project period are crystal structure determinations of three Pol II-factor complexes, including the pre-mRNA 5' capping enzyme, a Pol II specific phosphatase Fcp1 and a subassembly of the Pol II transcription preinitiation complex.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 转录是基因表达的第一步。 来自许多细胞过程的信号，例如营养感应，分解代谢产物抑制，激素刺激，分化和对环境刺激的反应最终达到转录水平以发挥其作用。 转录控制的像差是肿瘤发生的基本机制之一，因为癌症是由于增殖性和分化性遗传程序之间的良好平衡而引起的。 癌基产品经常被认为是信号转导，细胞周期和转录法规的控制网络中的关键参与者。 我们建议在酵母中检查各种Pol II因子复合物，对应于转录周期中不同的功能步骤。 将在10-亚基pol II和核糖体的工作中开发的生化和X射线晶体学方法，将采用，完善和扩展，以解决转录调节中新一代的结构问题。 在项目期间的具体目的是对三个POL II因子复合物的晶体结构确定，包括前MRNA 5'上限酶，POL II特异性磷酸酶FCP1和POL II转录前启动络合物的亚组装。