RECOGNITION EVENTS IN RNA INTERFERENCE

RNA 干扰中的识别事件

• 批准号: 7955106
• 负责人: DINSHAW J PATEL
• 金额: $ 0.73万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955106
• 关键词: Alternative Splicing; Binding; Computer Retrieval of Information on Scientific Projects Database; Elements; Event; Fingers; Funding; Grant; Institution; Lead; Myotonic Dystrophy; Nucleotides; Proteins; RNA; RNA Interference; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; programs; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Muscleblind-like (MBNL) proteins, regulators of developmentally programmed alternative splicing, harbor tandem CCCH Zn finger (ZnF) domains, that target pre-mRNAs containing YGCU(U/G)Y sequence elements. In myotonic dystrophy, reduced levels of MBNL proteins lead to aberrant alternative splicing of a subset of pre-mRNAs. To elucidate principles of the regulatory RNA elements recognition by MBNL we crystallized each MBNL1 tandem ZnF domain, ZnF1/2 and ZnF3/4, in the free form, and the structure of the second tandem ZnF3/4 bound to CGCUGU nucleotide.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 肌肉闪光（MBNL）蛋白质，发育编程的替代剪接的调节剂，Harbour Tandem CCCH Zn指（ZNF）域，其靶向含有YGCU（U/G）Y序列元件的Pre-MRNA。在肌发育症中，MBNL蛋白的水平降低导致一部分MRNA子集的异常替代剪接。为了阐明MBNL的调节RNA元件识别的原理，我们以自由形式结晶了每个MBNL1串联Znf域，Znf1/2和Znf3/4，以及第二个Tandem Znf3/4的结构与CGCUGU核苷酸结合。