ANIMAL CORE

动物核心

• 批准号: 7109129
• 负责人: William Edmund Sonntag
• 金额: $ 22.43万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7109129
• 关键词: aging; animal care; animal colony; behavior test; biomedical facility; brain metabolism; cerebrovascular system; cognition disorders; developmental neurobiology; disease /disorder model; insulinlike growth factor; laboratory rat; learning; memory; pituitary dwarfism; somatotropin

One of the primary factors in assessing specific mechanisms of brain aging is high quality animal care. One of the most common confounding variables in aging studies are inconsistent animal care, non-specific stress, and the presence of unrecognized age-related pathologies that compromise the dependent variables under investigation. For these reasons (and others), the Animal Core within the PPG must be of the highest quality. In the past funding period, this Core has been successful in maintaining animals in specific pathogen free (SPF) conditions for project studies, in developing and providing extensive validation of a novel model of adult-onset growth hormone deficiency that will continue to be used in the proposed funding period. This model provides a unique resource for the Program Project and opportunity to investigate the effects of growth hormone deficiency apart from other factors that may contribute to brain aging. The Animal Core has multiple aims: 1) The Core will procure and maintain animals used by program investigators and meet all AAALAC and institutional standards for animal care in the satellite facilities. The Core will also maintain a breeding colony of dw/+ (female) and dw/dw (male) Lewis rats and raise offspring to be used for the model of adult-onset growth hormone deficiency proposed in this application. The Core will coordinate animal/tissue use among the projects. 2) The Core personnel will assess basic parameters of aging animals and monitor sentinel and experimental animals for the presence of disease. 3) The Core will provide surgical services. 4) The Core will be responsible for irradiation of animals, as necessary, and measure hormone levels in tissue. 5) The Core will conduct behavioral analyses on animals to assess age-related alterations in hippocampally dependent measures of learning and memory and will develop frontal-lobe dependent tasks of learning and memory. The Animal Core is an essential aspect of the Program Project ensuring effective management of limited resources, smooth interactions between projects and the use of animal models that have been extensively validated for studies of the biology of aging.

评估大脑衰老特定机制的主要因素之一是高质量的动物护理。 衰老研究中最常见的混杂变量之一是动物护理不一致、非特异性 压力以及存在未识别的与年龄相关的病变，这些病变会损害因变量 正在调查中。由于这些原因（以及其他原因），PPG 内的动物核心必须是最高的 质量。在过去的资助期间，该核心已成功地将动物维持在特定的环境中 用于项目研究的无病原体（SPF）条件，开发和提供广泛的验证 成人发病的生长激素缺乏症的新模型将继续在拟议的资助中使用 时期。该模型为计划项目提供了独特的资源和调查的机会 除了其他可能导致大脑衰老的因素外，生长激素缺乏的影响。动物 核心有多个目标：1) 核心将采购和维护项目研究人员使用的动物， 符合卫星设施中动物护理的所有 AAALAC 和机构标准。核心还将 维持 dw/+（雌性）和 dw/dw（雄性）Lewis 大鼠的繁殖群体并饲养后代用于 本申请中提出的成人发病的生长激素缺乏症模型。核心将协调 项目中动物/组织的使用。 2）核心人员对衰老动物的基本参数进行评估 监测哨兵和实验动物是否存在疾病。 3) 核心将提供手术 服务。 4）核心将负责根据需要对动物进行照射，并测量激素 组织中的水平。 5) 核心将对动物进行行为分析，以评估与年龄相关的变化 海马依赖性学习和记忆测量，并将发展额叶依赖性任务 的学习和记忆。动物核心是该计划项目的一个重要方面，确保有效 有限资源的管理、项目之间的顺利互动以及动物模型的使用 已在衰老生物学研究中得到广泛验证。