Ink-jet Matrix Coating System for MALDI MS Imaging

用于 MALDI MS 成像的喷墨矩阵涂层系统

基本信息

批准号：
7270933
负责人：
Patrick W Cooley
金额：
$ 13.51万
依托单位：
MICROFAB TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-05 至 2008-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7270933
关键词：
4-coumaric acid Ablation Acetic Acid Acetic Acids Acids Address Alzheimer&apos s Disease Area Australia Automation Biological Markers Biomedical Research Brain Butanols Cardiac Chemicals Chills Classification Color Computer software Coupled Craniocerebral Trauma Crystallization DNA Dental Deposition Detection Development Devices Diagnosis Diagnostic Digestion Disease Drops Drug Compounding Drug Controls Drug Delivery Systems Drug Formulations Dyes Electronics Enzymes Ethanol Evaluation Film Freezing Frozen Sections Generations Glass Glucose Goals Gold Height Histocompatibility Testing Image Immersion Investigative Technique Implantable Pump In Situ Individual Ink Ions Japan Label Lasers Lateral Lipids Liquid substance Liver Localized MALDI-TOF Mass Spectrometry Maps Mass Spectrum Analysis Mechanics Membrane Membrane Proteins Methods Microdeposition Molecular Molecular Weight Motion Nerve Regeneration Neurodegenerative Disorders Nitrogen Noise Orthopedics Pathology Patients Pattern Peptides Pharmaceutical Preparations Phase Placement Plants Pliability Polymers Positioning Attribute Preclinical Drug Evaluation Preparation Printing Process Protein Analysis Proteins Proteome Purpose Range Reagent Recording of previous events Relative (related person)Research Activity Resolution Sample Size Sampling Signal Transduction Signaling Protein Smell Perception Solubility Solutions Solvents Spatial Distribution Specimen Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spottings Staging Stainless Steel Standards of Weights and Measures Stents Surface System Techniques Technology Testing Thick Tissue Engineering Tissue Sample Tissues Two-Dimensional Polyacrylamide Gel Electrophoresis Zinc base cryostat density design evaporation image processing improved indium tin oxide instrument interest mass spectrometer migration molecular imaging nanolitre outcome forecast prototype sinapinic acid surfactant tool vector

项目摘要

DESCRIPTION (provided by applicant): In this project, MicroFab will build a prototype system capable of depositing matrix solutions onto the tissue sections with high resolution using picoliter volumes (nanoliter volumes via the accretion of drops) for the purposes of MALDI MS imaging. The system will be very flexible in terms of amount of matrix deposited per spot, distribution of the matrix on the tissue sample, and the matrix spot pitch and placement. The system will provide capability for overprinting additional matrix solution and/or solvents and surfactants onto previously deposited matrix spots to improve analyte extraction from the protein sample. We will optimize the formulation of the matrix solutions to achieve the most reliable microdispensing, while maintaining functionality for the imaging process. The actual coating process will be verified by deposition onto tissue sections with evaluation by MALDI-TOF MS. The matrix coating platform is an automated tool for the precise microdeposition of matrix solutions onto tissue samples for MALDI MS imaging. The array of discrete matrix spots significantly reduces the lateral migration of protein on the surface of the tissue section, while increasing the analyte extraction per area, resulting in a greater resolved protein image.

描述（由申请人提供）：在该项目中，MicroFab 将构建一个原型系统，能够使用皮升体积（通过液滴积聚实现纳升体积）以高分辨率将基质溶液沉积到组织切片上，以进行 MALDI MS 成像。该系统在每个点沉积的基质量、基质在组织样本上的分布以及基质点间距和放置方面将非常灵活。该系统将提供将额外的基质溶液和/或溶剂和表面活性剂套印到先前沉积的基质点上的能力，以改善从蛋白质样品中提取分析物。我们将优化基质解决方案的配方，以实现最可靠的微点胶，同时保持成像过程的功能。实际的涂层过程将通过沉积到组织切片上并通过 MALDI-TOF MS 评估来验证。基质涂层平台是一种自动化工具，用于将基质溶液精确微沉积到组织样本上以进行 MALDI MS 成像。离散矩阵点阵列显着减少了组织切片表面上蛋白质的横向迁移，同时增加了每个区域的分析物提取量，从而获得分辨率更高的蛋白质图像。