DESIGN OF SELF-ASSEMBLED 3D DNA CRYSTALS USING 6HB

使用 6HB 设计自组装 3D DNA 晶体

• 批准号: 7957272
• 负责人: JENS J BIRKTOFT
• 金额: $ 1.88万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957272
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA; Devices; Diffusion; Electronics; Funding; Goals; Grant; Institution; Light; Methods; Molecular; Nanotechnology; Pattern; Positioning Attribute; Proteins; Research; Research Personnel; Resolution; Resources; Shapes; Source; Synchrotrons; System; United States National Institutes of Health; Work; X ray diffraction analysis; X-Ray Diffraction; base; design; macromolecule; nanoscale; success; three dimensional structure; vapor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structural DNA nanotechnology uses unusual DNA motifs to build target shapes and periodic arrangements. We expect these systems can be applied to several practical ends: The key motivating goal for this research is that spatially periodic networks are crystals. Also the Steepest challenge of the research is the construction of 3D crystals with high order. If we can build stick-figure crystalline cages in the nanometer scale, they could be used to orient other biological macromolecules as guests inside those cages, thereby rendering their 3D structure amenable to diffraction analysis. The globular shapes of proteins are not conducive to the packing alignment that is essential to form a well-ordered crystal, due to specific interactions between DNA and proteins, DNA 3D crystalline crystal is an excellent candidate for forming periodic crystalline cages that can host proteins in order for the X-ray diffraction studies; Similarly, the same crystalline arrays could be used to position and orient components of molecular electronic devices with nanometer-scale precision. A number of designs using unusual DNA motifs have been self-assembled to yield 3D crystals. Although we have been successfully build 2D arrays, the structural criteria of success with 2D arrangements are based typically on AFM observation with resolution limit of 3-10 nm. By contrast the goal for successful work of x-ray diffraction is around 2 ¿¿¿ . However the diffraction patterns of our preliminary crystals have been limited to 10 ¿¿¿ . Six-DNA helix-Bundle is the exact motif I¿¿¿¿¿"m using for this research. In this motif six DNA helices associate to form a hexagon arrangement if you look down the helical axis of DNA. We use thermo-control and vapour-diffusion methods. We have been successfully made 2D arrays by using the former method.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中央赠款提供的资源 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，在列出的机构中可能会被压制 对于中心，这不一定是调查员的工厂。 结构DNA纳米技术的靶向形状和周期性的安排。纳米尺度的逻辑大分子作为内部的笼子，因此，将3D结构适合于衍射分析。候选晶体晶体笼可以容纳蛋白质，以适合用于形式的X射线衍射研究。 许多设计不寻常的DNA基序是自组装以产生3D晶体的2D结构标准。衍射约为2€挥之不去挥之不去但是。挥之不去挥之不去六-DNA螺旋 - 捆绑包是确切的主题i。挥之不去挥之不去挥之不去挥之不去“如果您是O-Control和蒸气扩散方法的螺旋轴。