QUESTION OR TRAINING REQUEST FOR THE YEAST RESOURCE CENTER

对酵母资源中心的问题或培训请求

• 批准号: 7957701
• 负责人: Timothy G Formosa
• 金额: $ 0.48万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957701
• 关键词: Biology; Computer Retrieval of Information on Scientific Projects Database; Funding; Fungal Genome; Grant; Hybrids; Institution; Phosphotyrosine; Proteins; Research; Research Personnel; Resources; Source; Training; United States National Institutes of Health; Yeasts; interest; research study; src Homology Region 2 Domain; yeast protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in studying interactions with a structural domain of S cerevisiae Spt6 (the only known SH2 domain in yeast). Spt6 has several structural domains, so it is not clear that previous experiments that used the whole Spt6 protein will be relevant. As yeast are not known to have phosphotyrosine, it is of interest to know what this SH2 domain interacts with. Is the YRC an appropriate collaborator for a 2-hybrid screen with a subdomain of a yeast protein like this?

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们有兴趣研究与酿酒酵母 Spt6（酵母中唯一已知的 SH2 结构域）结构域的相互作用。 Spt6 具有多个结构域，因此尚不清楚之前使用整个 Spt6 蛋白的实验是否相关。由于未知酵母具有磷酸酪氨酸，因此有必要了解该 SH2 结构域与什么相互作用。 YRC 是否是进行具有此类酵母蛋白子结构域的 2 杂交筛选的合适合作者？