STRUCTURAL BIOLOGY OF VIRAL AND BACTERIAL VIRULENCE FACTORS

病毒和细菌毒力因子的结构生物学

• 批准号: 8170156
• 负责人: Kenneth Ng
• 金额: $ 0.51万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170156
• 关键词: Anabolism; Antiviral Agents; Avidity; Binding; Carbohydrates; Catalysis; Cell Wall; Clostridium difficile; Complex; Computer Retrieval of Information on Scientific Projects Database; Elements; Enzymes; Funding; Grant; Infection; Institution; Mycobacterium tuberculosis; Norwalk virus; Pathogenesis; Phase; Polymerase; Research; Research Personnel; Resources; Source; Specificity; Structure; System; Time; Toxin; United States National Institutes of Health; Viral; Virulence Factors; Work; base; carbohydrate receptor; drug development; novel; receptor; receptor binding; structural biology; Anabolism; Antiviral Agents; Avidity; Binding; Carbohydrates; Catalysis; Cell Wall; Clostridium difficile; Complex; Computer Retrieval of Information on Scientific Projects Database; Elements; Enzymes; Funding; Grant; Infection; Institution; Mycobacterium tuberculosis; Norwalk virus; Pathogenesis; Phase; Polymerase; Research; Research Personnel; Resources; Source; Specificity; Structure; System; Time; Toxin; United States National Institutes of Health; Viral; Virulence Factors; Work; base; carbohydrate receptor; drug development; novel; receptor; receptor binding; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have several projects investigating the structure and function of key enzymes and other virulence factors involved with viral and bacterial pathogenesis. Our recent structure determination of Norwalk virus polymerase replication complexes reveal several novel features that demand further structural work to determine more precisely the mechanisms underlying catalysis and inhibition in this key target for antiviral drug development. Our recent structure determination of receptor-binding fragments from Clostridium difficile toxins bound to carbohydrate receptors showed for the first time the structural basis underlying receptor recognition. Further structural work is needed in this system to define the elements underlying the specificity and avidity of carbohydrate recognition that will provide the basis for developing novel treatments for C. difficile infections. Newer projects on enzymes important for the biosynthesis of cell wall carbohydrates in Mycobacterium tuberculosis have yielded crystals that may need MAD or SAD phasing to assist with structure determination.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们有几个项目，研究了关键酶的结构和功能以及与病毒和细菌发病机理有关的其他毒力因子。 我们最近对Norwalk病毒聚合酶复制络合物的结构确定揭示了一些新的特征，这些新特征需要进一步的结构工作，以更确切地确定催化和抑制抗病毒药物开发的基础机制。 我们最近对与碳水化合物受体结合的艰难梭菌毒素的受体结合片段的测定首次表现出结构性基础受体识别。 在该系统中需要进一步的结构性工作来定义碳水化合物识别的特异性和亲和力的元素，这将为开发用于艰难梭菌感染的新型治疗方法提供基础。 关于结核分枝杆菌中细胞壁碳水化合物的生物合成重要的酶的较新项目，产生了可能需要疯狂或悲伤的阶段以帮助确定结构的晶体。