ROLE OF YPT GTPASES IN INTRACELLULAR TRAFFICKING

YPT GTASE 在细胞内贩运中的作用

• 批准号: 7957793
• 负责人: Nava Segev
• 金额: $ 1.63万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957793
• 关键词: Biology; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Grant; Guanosine Triphosphate; Guanosine Triphosphate Phosphohydrolases; Institution; Mediating; Research; Research Personnel; Resources; Role; Secretory Vesicles; Source; United States National Institutes of Health; rab GTP-Binding Proteins; trafficking; trans-Golgi Network

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ypt/Rab GTPases are conserved key regulators of all steps of intracellular trafficking. They act by cycling between the GTP "on" and the GDP "off" states. This cycling is regulated in turn by upstream regulators: GEFs and GAPs. At the "on" state Ypt/Rabs interact with effectors that mediate vesicular transport. We focus on Ypt31/32, Ypt/Rab GTPases responsible for secretory vesicle exit from trans-Golgi network, looking for their effectors and their mode of action on these effectors, as well as their upstream regulators. We would like to use FRET for these purposes.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 YPT/RAB GTPases是细胞内贩运所有步骤的保守关键调节剂。 它们通过在GTP“ ON”和GDP“ Off”状态之间骑自行车而行动。 这种循环又受到上游调节剂的调节：GEFS和GAPS。 在“ ON”状态YPT/RABS与介导水泡转运的效应子相互作用。 我们专注于YPT31/32，YPT/RAB GTP酶，负责从跨性别网络退出的分泌囊泡，寻找其效应子及其对这些效应子的作用方式以及上游调节器。我们想将FRET用于这些目的。