Mouse Modeling Core

鼠标建模核心

基本信息

批准号：
7318143
负责人：
KEVIN G OSTEEN
金额：
--
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2012-03-31
项目状态：
已结题

项目摘要

Introduction to Revised Core C: We greatly appreciate the positive comments of the reviewers regarding the Mouse Modeling Core facility. We have responded to the issues raised, as outlined below, and would also like to draw attention an important addition to our Core services. We have very recently described a new experimental endometriosis model, using Rag2y(c) mice (Bruner-Tran et al, 2006b). Due to the severity of the immune defects of this immunosuppressed mouse strain, we have been able to transfer both human immune and endometrial cells to this animal, allowing examination of the potential role of immune cells in the pathophysiology of endometriosis. Although this model has not yet been fully characterized, we anticipate completing these studies within the next 12 months and making this model available to Center investigators immediately thereafter. We believe this model system will be a valuable addition to the Mouse Modeling Core which should aid our understanding both the development and treatment of endometriosis. ¿ 1. Past experience or a plan describing assessment of successful engraftment of shipped samples was not discussed. This information has now been included within the Core description. Specifically, the Core lab has previously examined the effect of overnight shipment of fresh tissues prior to engraftment into mice and we have found no noticeable difference between size or number of lesions compared to tissues that were not shipped. Additionally, we have previously demonstrated that tissues can be pre-cultured for up to 48 hrs (versus the typical 24 hrs) without noticeable impact on cell survival or lesion implantation rate further supporting the feasibility of shipping tissues overnight and then culturing 24 hrs prior to introduction into mice. 2. Communication between the PLs at Yale and the Core lab will be critical and should be discussed in more detail. As now detailed within the Core description, Drs. Osteen and Bruner-Tran have extensive experience conducting studies as an "off-site" location for our collaborators. We foresee no difficulties in working with investigators at Yale. 3. There was little discussion regarding the role of internal and external review groups in oversight of the Core. External review will be conducted twice each year, alternating between Vanderbilt and Yale. Yale's internal review group will additionally visit the Vanderbilt sites (the Mouse Core and Dr. Osteen's laboratory) at regular intervals. Highlighting of Changes/Corrections: Sections of this application which are significantly altered from the previous submission have been indicated by the presence of a bold line to the left of the text, as present to the left of this paragraph.

修订后的核心 C 简介：我们非常感谢审稿人的积极评论关于鼠标建模核心设施，我们已对提出的问题做出了回应，如下所述：还想提请注意我们最近对核心服务的重要补充。描述了一种新的实验性子宫内膜异位症模型，使用 Rag2y(c) 小鼠（Bruner-Tran 等人，2006b）。鉴于这种免疫抑制小鼠品系的免疫缺陷的严重程度，我们已经能够将人类免疫细胞和子宫内膜细胞转移到该动物体内，从而检查其潜力尽管该模型尚未完全阐明，但免疫细胞在子宫内膜异位症的病理生理学中的作用。根据特征，我们预计在未来 12 个月内进行这些研究，并制作该模型我们相信此模型系统将立即提供给中心研究人员。除了鼠标建模核心之外，它应该有助于我们理解开发和 ¿治疗子宫内膜异位症。 1. 过去的经验或描述评估已运送的成功植入的计划示例尚未讨论。此信息现已包含在核心描述中。具体来说，核心实验室之前已经检查了在之前过夜运输新鲜组织的影响。植入小鼠体内，我们发现病变的大小或数量没有明显差异与未发货的纸巾相比。可以预培养长达 48 小时（相对于典型的 24 小时），而不会对细胞存活产生明显影响或病变植入率进一步支持过夜运输组织然后培养的可行性在引入小鼠之前24小时。 2. 耶鲁大学 PL 和核心实验室之间的沟通至关重要，并且应该正如现在在核心描述中详细讨论的那样，Osteen 和 Bruner-Tran 博士。我们预计，我们的合作者拥有作为“异地”地点开展研究的丰富经验。与耶鲁大学的研究人员合作没有任何困难。 3. 关于内部和外部审查小组的作用的讨论很少。核心的外部审查每年进行两次，轮流进行。范德比尔特大学和耶鲁大学的内部审查小组还将访问范德比尔特大学网站（鼠标）。 Core 和 Osteen 博士的实验室）定期进行。突出显示更改/更正：本应用程序中重要的部分来自先前提交的内容已通过文本左侧的粗线表示，如下所示出现在本段左侧。