Animal Models

动物模型

基本信息

批准号：
7727454
负责人：
Keith L. Knutson
金额：
$ 18.89万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2014-06-30
项目状态：
已结题

项目摘要

instmctions): The purpose of the proposed Mayo Clinic Ovarian SPORE is to sfimulate and facilitate rigorous translational research in ovarian cancerwork that will take new basic and population science discoveries and convert them to improved intervenfions for women with ovarian cancer. Three of the four projects in this SPORE grant rely heavily on this proposed Animal Models Core for the evaluafion of novel interventions (imaging and therapeufics) and for refining experimental approaches and delivery regimens in ovarian cancer-bearing mice. To ensure that all the animal experimentafion can be performed in an expert and efficient manner, and that SPORE investigators use standarized models for their varying therapeutic strategiesthe Animal Core will serve as a central resource and, in collaboration with the laboratory personnel from each project, will perform all the animal experimentafion described in the projects. Specifically, the Animal Core will: (1) ensure the efficient planning, piurchase and ufilizafion of experimental mice, (2) provide the necessary facilifies and animal handling expertise for the projects; (3) provide xenograft and immunocompetent mouse models for testing of novel agents and therapeutic strategies and (4) provide veterinary expertise and access to non-invasive imaging strategies to monitor tumor burden. Importantly, the Animal Models Core will assure the following: (1) Consistency in animal modeling. Modeling through this core will maintain consistency in methods and outcomes, making it easier to reproduce our findings and easier to combine approaches from other labs; (2) Provision of strong animal modeling expertise to all projects. While all project teams have had experience with animal models, some have less than others. By providing a core, we can ensure that all SPORE members will have access to the highest level of skills available; (3) Opfimal interaction across projects with regard to modeling strategies. The core will bring together the investigators from each project and provide an outstanding avenue for new collaborations and the strengthening of existing collaborafions; (4) Efficiency in animal use and care. This core will result in an organized approach to animal use, minimizing waste and the numbers of animals used and eliminafing redundancy in personnel. RELEVANCE (See instructions): The Animal Models core will develop and maintain murine models of ovarian cancer. These models are necessary for understanding the complex interacfions of ovarian cancer with normal healthy tissues and the immune system. Further, the models are needed for the development of novel therapeutics that require animal tesfing prior to human clinical use.

指令）：拟议的梅奥诊所卵巢孢子的目的是刺激和促进严格的转化卵巢癌工作的研究将采用新的基础和人口科学发现并将其转化为他们改善了对卵巢癌女性的干预措施。 SPORE 中的四个项目中的三个拨款很大程度上依赖于拟议的动物模型核心来评估新的干预措施（成像和治疗），并用于改进卵巢癌携带者的实验方法和给药方案老鼠。为了确保所有的动物实验能够以专业、高效的方式进行， SPORE 研究人员使用标准化模型来制定不同的治疗策略 Core 将作为中央资源，并与每个项目的实验室人员合作，将进行项目中描述的所有动物实验。具体来说，动物核心将：(1) 确保实验小鼠的有效规划、购买和利用，（2）提供必要的为项目提供便利和动物处理专业知识； (3) 提供异种移植和免疫活性小鼠用于测试新药和治疗策略的模型，以及（4）提供兽医专业知识和渠道监测肿瘤负荷的非侵入性成像策略。重要的是，动物模型核心将确保以下：（1）动物模型的一致性。通过这个核心建模将保持一致性方法和结果，使我们更容易重现我们的发现，并更容易结合来自其他实验室； (2) 为所有项目提供强大的动物建模专业知识。虽然所有项目团队都已经动物模型的经验，有些比其他的少。通过提供核心，我们可以确保所有 SPORE 成员将能够获得最高水平的技能； (3) 最优交互有关建模策略的项目。核心将汇集每个项目的研究人员并为新的合作和加强现有的合作提供良好的途径； (4) 动物使用和护理的效率。这个核心将导致有组织的动物使用方法，最大限度地减少浪费和使用的动物数量，并消除人员冗余。相关性（参见说明）：动物模型核心将开发和维持卵巢癌的小鼠模型。这些型号是对于了解卵巢癌与正常健康组织的复杂相互作用以及免疫系统。此外，需要这些模型来开发新的疗法，这些疗法需要在人类临床使用之前进行动物测试。